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Structural and Functional Analysis of BBA03, Borrelia burgdorferi Competitive Advantage Promoting Outer Surface Lipoprotein

BBA03 is a Borrelia burgdorferi outer surface lipoprotein encoded on one of the most conserved plasmids in Borrelia genome, linear plasmid 54 (lp54). Although many of its genes have been identified as contributing or essential for spirochete fitness in vivo, the majority of the proteins encoded on t...

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Autores principales: Fridmanis, Jēkabs, Bobrovs, Raitis, Brangulis, Kalvis, Tārs, Kaspars, Jaudzems, Kristaps
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7650648/
https://www.ncbi.nlm.nih.gov/pubmed/33050189
http://dx.doi.org/10.3390/pathogens9100826
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author Fridmanis, Jēkabs
Bobrovs, Raitis
Brangulis, Kalvis
Tārs, Kaspars
Jaudzems, Kristaps
author_facet Fridmanis, Jēkabs
Bobrovs, Raitis
Brangulis, Kalvis
Tārs, Kaspars
Jaudzems, Kristaps
author_sort Fridmanis, Jēkabs
collection PubMed
description BBA03 is a Borrelia burgdorferi outer surface lipoprotein encoded on one of the most conserved plasmids in Borrelia genome, linear plasmid 54 (lp54). Although many of its genes have been identified as contributing or essential for spirochete fitness in vivo, the majority of the proteins encoded on this plasmid have no known function and lack homologs in other organisms. In this paper, we report the solution NMR structure of the B. burgdorferi outer surface lipoprotein BBA03, which is known to provide a competitive advantage to the bacteria during the transmission from tick vector to mammalian host. BBA03 shows structural homology to other outer surface lipoproteins reflecting their genetic and evolutionary relatedness. Analysis of the structure reveals a pore in BBA03, which could potentially bind lipids.
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spelling pubmed-76506482020-11-10 Structural and Functional Analysis of BBA03, Borrelia burgdorferi Competitive Advantage Promoting Outer Surface Lipoprotein Fridmanis, Jēkabs Bobrovs, Raitis Brangulis, Kalvis Tārs, Kaspars Jaudzems, Kristaps Pathogens Article BBA03 is a Borrelia burgdorferi outer surface lipoprotein encoded on one of the most conserved plasmids in Borrelia genome, linear plasmid 54 (lp54). Although many of its genes have been identified as contributing or essential for spirochete fitness in vivo, the majority of the proteins encoded on this plasmid have no known function and lack homologs in other organisms. In this paper, we report the solution NMR structure of the B. burgdorferi outer surface lipoprotein BBA03, which is known to provide a competitive advantage to the bacteria during the transmission from tick vector to mammalian host. BBA03 shows structural homology to other outer surface lipoproteins reflecting their genetic and evolutionary relatedness. Analysis of the structure reveals a pore in BBA03, which could potentially bind lipids. MDPI 2020-10-09 /pmc/articles/PMC7650648/ /pubmed/33050189 http://dx.doi.org/10.3390/pathogens9100826 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fridmanis, Jēkabs
Bobrovs, Raitis
Brangulis, Kalvis
Tārs, Kaspars
Jaudzems, Kristaps
Structural and Functional Analysis of BBA03, Borrelia burgdorferi Competitive Advantage Promoting Outer Surface Lipoprotein
title Structural and Functional Analysis of BBA03, Borrelia burgdorferi Competitive Advantage Promoting Outer Surface Lipoprotein
title_full Structural and Functional Analysis of BBA03, Borrelia burgdorferi Competitive Advantage Promoting Outer Surface Lipoprotein
title_fullStr Structural and Functional Analysis of BBA03, Borrelia burgdorferi Competitive Advantage Promoting Outer Surface Lipoprotein
title_full_unstemmed Structural and Functional Analysis of BBA03, Borrelia burgdorferi Competitive Advantage Promoting Outer Surface Lipoprotein
title_short Structural and Functional Analysis of BBA03, Borrelia burgdorferi Competitive Advantage Promoting Outer Surface Lipoprotein
title_sort structural and functional analysis of bba03, borrelia burgdorferi competitive advantage promoting outer surface lipoprotein
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7650648/
https://www.ncbi.nlm.nih.gov/pubmed/33050189
http://dx.doi.org/10.3390/pathogens9100826
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