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Structural and Functional Analysis of BBA03, Borrelia burgdorferi Competitive Advantage Promoting Outer Surface Lipoprotein
BBA03 is a Borrelia burgdorferi outer surface lipoprotein encoded on one of the most conserved plasmids in Borrelia genome, linear plasmid 54 (lp54). Although many of its genes have been identified as contributing or essential for spirochete fitness in vivo, the majority of the proteins encoded on t...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7650648/ https://www.ncbi.nlm.nih.gov/pubmed/33050189 http://dx.doi.org/10.3390/pathogens9100826 |
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author | Fridmanis, Jēkabs Bobrovs, Raitis Brangulis, Kalvis Tārs, Kaspars Jaudzems, Kristaps |
author_facet | Fridmanis, Jēkabs Bobrovs, Raitis Brangulis, Kalvis Tārs, Kaspars Jaudzems, Kristaps |
author_sort | Fridmanis, Jēkabs |
collection | PubMed |
description | BBA03 is a Borrelia burgdorferi outer surface lipoprotein encoded on one of the most conserved plasmids in Borrelia genome, linear plasmid 54 (lp54). Although many of its genes have been identified as contributing or essential for spirochete fitness in vivo, the majority of the proteins encoded on this plasmid have no known function and lack homologs in other organisms. In this paper, we report the solution NMR structure of the B. burgdorferi outer surface lipoprotein BBA03, which is known to provide a competitive advantage to the bacteria during the transmission from tick vector to mammalian host. BBA03 shows structural homology to other outer surface lipoproteins reflecting their genetic and evolutionary relatedness. Analysis of the structure reveals a pore in BBA03, which could potentially bind lipids. |
format | Online Article Text |
id | pubmed-7650648 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76506482020-11-10 Structural and Functional Analysis of BBA03, Borrelia burgdorferi Competitive Advantage Promoting Outer Surface Lipoprotein Fridmanis, Jēkabs Bobrovs, Raitis Brangulis, Kalvis Tārs, Kaspars Jaudzems, Kristaps Pathogens Article BBA03 is a Borrelia burgdorferi outer surface lipoprotein encoded on one of the most conserved plasmids in Borrelia genome, linear plasmid 54 (lp54). Although many of its genes have been identified as contributing or essential for spirochete fitness in vivo, the majority of the proteins encoded on this plasmid have no known function and lack homologs in other organisms. In this paper, we report the solution NMR structure of the B. burgdorferi outer surface lipoprotein BBA03, which is known to provide a competitive advantage to the bacteria during the transmission from tick vector to mammalian host. BBA03 shows structural homology to other outer surface lipoproteins reflecting their genetic and evolutionary relatedness. Analysis of the structure reveals a pore in BBA03, which could potentially bind lipids. MDPI 2020-10-09 /pmc/articles/PMC7650648/ /pubmed/33050189 http://dx.doi.org/10.3390/pathogens9100826 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Fridmanis, Jēkabs Bobrovs, Raitis Brangulis, Kalvis Tārs, Kaspars Jaudzems, Kristaps Structural and Functional Analysis of BBA03, Borrelia burgdorferi Competitive Advantage Promoting Outer Surface Lipoprotein |
title | Structural and Functional Analysis of BBA03, Borrelia burgdorferi Competitive Advantage Promoting Outer Surface Lipoprotein |
title_full | Structural and Functional Analysis of BBA03, Borrelia burgdorferi Competitive Advantage Promoting Outer Surface Lipoprotein |
title_fullStr | Structural and Functional Analysis of BBA03, Borrelia burgdorferi Competitive Advantage Promoting Outer Surface Lipoprotein |
title_full_unstemmed | Structural and Functional Analysis of BBA03, Borrelia burgdorferi Competitive Advantage Promoting Outer Surface Lipoprotein |
title_short | Structural and Functional Analysis of BBA03, Borrelia burgdorferi Competitive Advantage Promoting Outer Surface Lipoprotein |
title_sort | structural and functional analysis of bba03, borrelia burgdorferi competitive advantage promoting outer surface lipoprotein |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7650648/ https://www.ncbi.nlm.nih.gov/pubmed/33050189 http://dx.doi.org/10.3390/pathogens9100826 |
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