Cargando…
Emergence of mcr-3 carrying Escherichia coli in Diseased Pigs in South Korea
We examined the prevalence and molecular characteristics of mcr-3 carrying colistin-resistant Escherichia coli among cattle, pig, and chicken isolates in South Korea. Among a total of 185 colistin-resistant E. coli isolates determined in this study (47 from cattle, 90 from pigs, and 48 from chicken)...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7650651/ https://www.ncbi.nlm.nih.gov/pubmed/33036214 http://dx.doi.org/10.3390/microorganisms8101538 |
_version_ | 1783607525853102080 |
---|---|
author | Mechesso, Abraham Fikru Moon, Dong Chan Kang, Hee Young Song, Hyun-Ju Kim, Su-Jeong Choi, Ji-Hyun Kim, Mi Hyun Na, Seok Hyeon Kim, Ha-Young Jung, Byeong Yeal Yoon, Soon-Seek Lim, Suk-Kyung |
author_facet | Mechesso, Abraham Fikru Moon, Dong Chan Kang, Hee Young Song, Hyun-Ju Kim, Su-Jeong Choi, Ji-Hyun Kim, Mi Hyun Na, Seok Hyeon Kim, Ha-Young Jung, Byeong Yeal Yoon, Soon-Seek Lim, Suk-Kyung |
author_sort | Mechesso, Abraham Fikru |
collection | PubMed |
description | We examined the prevalence and molecular characteristics of mcr-3 carrying colistin-resistant Escherichia coli among cattle, pig, and chicken isolates in South Korea. Among a total of 185 colistin-resistant E. coli isolates determined in this study (47 from cattle, 90 from pigs, and 48 from chicken), PCR amplification detected mcr-3 genes in 17 isolates predominantly from diseased pigs. The mcr-3 genes were characterized as mcr-3.1 in 15 isolates and mcr-3.5 in 2 isolates. The mcr-3 gene was transferred to the E. coli J53 recipient strain from more than 50% of the mcr-3-carrying isolates. The mcr-3.1 and mcr-3.5 genes were identified predominantly in IncHI2 and IncP plasmids, respectively. Multi-locus sequence typing analysis revealed eight previously reported sequence types (ST), including ST1, ST10, and ST42. We identified isolates with similar pulsed-field gel electrophoresis patterns from diseased pigs in three farms. Besides, the isolates carried various virulence factors and demonstrated resistance to multiple antimicrobials, including β-lactams and quinolones. Further, the mcr-3.5 encodes three amino acid substitutions compared with mcr-3.1. To the best of our knowledge, this is the first report of pathogenic E. coli carrying mcr-3.5 in South Korea, which implies that mcr-3 variants may have already been widely spread in the pig industry. |
format | Online Article Text |
id | pubmed-7650651 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76506512020-11-10 Emergence of mcr-3 carrying Escherichia coli in Diseased Pigs in South Korea Mechesso, Abraham Fikru Moon, Dong Chan Kang, Hee Young Song, Hyun-Ju Kim, Su-Jeong Choi, Ji-Hyun Kim, Mi Hyun Na, Seok Hyeon Kim, Ha-Young Jung, Byeong Yeal Yoon, Soon-Seek Lim, Suk-Kyung Microorganisms Communication We examined the prevalence and molecular characteristics of mcr-3 carrying colistin-resistant Escherichia coli among cattle, pig, and chicken isolates in South Korea. Among a total of 185 colistin-resistant E. coli isolates determined in this study (47 from cattle, 90 from pigs, and 48 from chicken), PCR amplification detected mcr-3 genes in 17 isolates predominantly from diseased pigs. The mcr-3 genes were characterized as mcr-3.1 in 15 isolates and mcr-3.5 in 2 isolates. The mcr-3 gene was transferred to the E. coli J53 recipient strain from more than 50% of the mcr-3-carrying isolates. The mcr-3.1 and mcr-3.5 genes were identified predominantly in IncHI2 and IncP plasmids, respectively. Multi-locus sequence typing analysis revealed eight previously reported sequence types (ST), including ST1, ST10, and ST42. We identified isolates with similar pulsed-field gel electrophoresis patterns from diseased pigs in three farms. Besides, the isolates carried various virulence factors and demonstrated resistance to multiple antimicrobials, including β-lactams and quinolones. Further, the mcr-3.5 encodes three amino acid substitutions compared with mcr-3.1. To the best of our knowledge, this is the first report of pathogenic E. coli carrying mcr-3.5 in South Korea, which implies that mcr-3 variants may have already been widely spread in the pig industry. MDPI 2020-10-06 /pmc/articles/PMC7650651/ /pubmed/33036214 http://dx.doi.org/10.3390/microorganisms8101538 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Mechesso, Abraham Fikru Moon, Dong Chan Kang, Hee Young Song, Hyun-Ju Kim, Su-Jeong Choi, Ji-Hyun Kim, Mi Hyun Na, Seok Hyeon Kim, Ha-Young Jung, Byeong Yeal Yoon, Soon-Seek Lim, Suk-Kyung Emergence of mcr-3 carrying Escherichia coli in Diseased Pigs in South Korea |
title | Emergence of mcr-3 carrying Escherichia coli in Diseased Pigs in South Korea |
title_full | Emergence of mcr-3 carrying Escherichia coli in Diseased Pigs in South Korea |
title_fullStr | Emergence of mcr-3 carrying Escherichia coli in Diseased Pigs in South Korea |
title_full_unstemmed | Emergence of mcr-3 carrying Escherichia coli in Diseased Pigs in South Korea |
title_short | Emergence of mcr-3 carrying Escherichia coli in Diseased Pigs in South Korea |
title_sort | emergence of mcr-3 carrying escherichia coli in diseased pigs in south korea |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7650651/ https://www.ncbi.nlm.nih.gov/pubmed/33036214 http://dx.doi.org/10.3390/microorganisms8101538 |
work_keys_str_mv | AT mechessoabrahamfikru emergenceofmcr3carryingescherichiacoliindiseasedpigsinsouthkorea AT moondongchan emergenceofmcr3carryingescherichiacoliindiseasedpigsinsouthkorea AT kangheeyoung emergenceofmcr3carryingescherichiacoliindiseasedpigsinsouthkorea AT songhyunju emergenceofmcr3carryingescherichiacoliindiseasedpigsinsouthkorea AT kimsujeong emergenceofmcr3carryingescherichiacoliindiseasedpigsinsouthkorea AT choijihyun emergenceofmcr3carryingescherichiacoliindiseasedpigsinsouthkorea AT kimmihyun emergenceofmcr3carryingescherichiacoliindiseasedpigsinsouthkorea AT naseokhyeon emergenceofmcr3carryingescherichiacoliindiseasedpigsinsouthkorea AT kimhayoung emergenceofmcr3carryingescherichiacoliindiseasedpigsinsouthkorea AT jungbyeongyeal emergenceofmcr3carryingescherichiacoliindiseasedpigsinsouthkorea AT yoonsoonseek emergenceofmcr3carryingescherichiacoliindiseasedpigsinsouthkorea AT limsukkyung emergenceofmcr3carryingescherichiacoliindiseasedpigsinsouthkorea |