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Emergence of mcr-3 carrying Escherichia coli in Diseased Pigs in South Korea

We examined the prevalence and molecular characteristics of mcr-3 carrying colistin-resistant Escherichia coli among cattle, pig, and chicken isolates in South Korea. Among a total of 185 colistin-resistant E. coli isolates determined in this study (47 from cattle, 90 from pigs, and 48 from chicken)...

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Autores principales: Mechesso, Abraham Fikru, Moon, Dong Chan, Kang, Hee Young, Song, Hyun-Ju, Kim, Su-Jeong, Choi, Ji-Hyun, Kim, Mi Hyun, Na, Seok Hyeon, Kim, Ha-Young, Jung, Byeong Yeal, Yoon, Soon-Seek, Lim, Suk-Kyung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7650651/
https://www.ncbi.nlm.nih.gov/pubmed/33036214
http://dx.doi.org/10.3390/microorganisms8101538
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author Mechesso, Abraham Fikru
Moon, Dong Chan
Kang, Hee Young
Song, Hyun-Ju
Kim, Su-Jeong
Choi, Ji-Hyun
Kim, Mi Hyun
Na, Seok Hyeon
Kim, Ha-Young
Jung, Byeong Yeal
Yoon, Soon-Seek
Lim, Suk-Kyung
author_facet Mechesso, Abraham Fikru
Moon, Dong Chan
Kang, Hee Young
Song, Hyun-Ju
Kim, Su-Jeong
Choi, Ji-Hyun
Kim, Mi Hyun
Na, Seok Hyeon
Kim, Ha-Young
Jung, Byeong Yeal
Yoon, Soon-Seek
Lim, Suk-Kyung
author_sort Mechesso, Abraham Fikru
collection PubMed
description We examined the prevalence and molecular characteristics of mcr-3 carrying colistin-resistant Escherichia coli among cattle, pig, and chicken isolates in South Korea. Among a total of 185 colistin-resistant E. coli isolates determined in this study (47 from cattle, 90 from pigs, and 48 from chicken), PCR amplification detected mcr-3 genes in 17 isolates predominantly from diseased pigs. The mcr-3 genes were characterized as mcr-3.1 in 15 isolates and mcr-3.5 in 2 isolates. The mcr-3 gene was transferred to the E. coli J53 recipient strain from more than 50% of the mcr-3-carrying isolates. The mcr-3.1 and mcr-3.5 genes were identified predominantly in IncHI2 and IncP plasmids, respectively. Multi-locus sequence typing analysis revealed eight previously reported sequence types (ST), including ST1, ST10, and ST42. We identified isolates with similar pulsed-field gel electrophoresis patterns from diseased pigs in three farms. Besides, the isolates carried various virulence factors and demonstrated resistance to multiple antimicrobials, including β-lactams and quinolones. Further, the mcr-3.5 encodes three amino acid substitutions compared with mcr-3.1. To the best of our knowledge, this is the first report of pathogenic E. coli carrying mcr-3.5 in South Korea, which implies that mcr-3 variants may have already been widely spread in the pig industry.
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spelling pubmed-76506512020-11-10 Emergence of mcr-3 carrying Escherichia coli in Diseased Pigs in South Korea Mechesso, Abraham Fikru Moon, Dong Chan Kang, Hee Young Song, Hyun-Ju Kim, Su-Jeong Choi, Ji-Hyun Kim, Mi Hyun Na, Seok Hyeon Kim, Ha-Young Jung, Byeong Yeal Yoon, Soon-Seek Lim, Suk-Kyung Microorganisms Communication We examined the prevalence and molecular characteristics of mcr-3 carrying colistin-resistant Escherichia coli among cattle, pig, and chicken isolates in South Korea. Among a total of 185 colistin-resistant E. coli isolates determined in this study (47 from cattle, 90 from pigs, and 48 from chicken), PCR amplification detected mcr-3 genes in 17 isolates predominantly from diseased pigs. The mcr-3 genes were characterized as mcr-3.1 in 15 isolates and mcr-3.5 in 2 isolates. The mcr-3 gene was transferred to the E. coli J53 recipient strain from more than 50% of the mcr-3-carrying isolates. The mcr-3.1 and mcr-3.5 genes were identified predominantly in IncHI2 and IncP plasmids, respectively. Multi-locus sequence typing analysis revealed eight previously reported sequence types (ST), including ST1, ST10, and ST42. We identified isolates with similar pulsed-field gel electrophoresis patterns from diseased pigs in three farms. Besides, the isolates carried various virulence factors and demonstrated resistance to multiple antimicrobials, including β-lactams and quinolones. Further, the mcr-3.5 encodes three amino acid substitutions compared with mcr-3.1. To the best of our knowledge, this is the first report of pathogenic E. coli carrying mcr-3.5 in South Korea, which implies that mcr-3 variants may have already been widely spread in the pig industry. MDPI 2020-10-06 /pmc/articles/PMC7650651/ /pubmed/33036214 http://dx.doi.org/10.3390/microorganisms8101538 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Mechesso, Abraham Fikru
Moon, Dong Chan
Kang, Hee Young
Song, Hyun-Ju
Kim, Su-Jeong
Choi, Ji-Hyun
Kim, Mi Hyun
Na, Seok Hyeon
Kim, Ha-Young
Jung, Byeong Yeal
Yoon, Soon-Seek
Lim, Suk-Kyung
Emergence of mcr-3 carrying Escherichia coli in Diseased Pigs in South Korea
title Emergence of mcr-3 carrying Escherichia coli in Diseased Pigs in South Korea
title_full Emergence of mcr-3 carrying Escherichia coli in Diseased Pigs in South Korea
title_fullStr Emergence of mcr-3 carrying Escherichia coli in Diseased Pigs in South Korea
title_full_unstemmed Emergence of mcr-3 carrying Escherichia coli in Diseased Pigs in South Korea
title_short Emergence of mcr-3 carrying Escherichia coli in Diseased Pigs in South Korea
title_sort emergence of mcr-3 carrying escherichia coli in diseased pigs in south korea
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7650651/
https://www.ncbi.nlm.nih.gov/pubmed/33036214
http://dx.doi.org/10.3390/microorganisms8101538
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