Cargando…

Combined Supra- and Sub-Lesional Epidural Electrical Stimulation for Restoration of the Motor Functions after Spinal Cord Injury in Mini Pigs

This study evaluates the effect of combined epidural electrical stimulation (EES) applied above (C5) and below (L2) the spinal cord injury (SCI) at T8–9 combined with motor training on the restoration of sensorimotor function in mini pigs. The motor evoked potentials (MEP) induced by EES applied at...

Descripción completa

Detalles Bibliográficos
Autores principales: Fadeev, Filip, Eremeev, Anton, Bashirov, Farid, Shevchenko, Roman, Izmailov, Andrei, Markosyan, Vage, Sokolov, Mikhail, Kalistratova, Julia, Khalitova, Anastasiia, Garifulin, Ravil, Islamov, Rustem, Lavrov, Igor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7650717/
https://www.ncbi.nlm.nih.gov/pubmed/33081405
http://dx.doi.org/10.3390/brainsci10100744
_version_ 1783607541432844288
author Fadeev, Filip
Eremeev, Anton
Bashirov, Farid
Shevchenko, Roman
Izmailov, Andrei
Markosyan, Vage
Sokolov, Mikhail
Kalistratova, Julia
Khalitova, Anastasiia
Garifulin, Ravil
Islamov, Rustem
Lavrov, Igor
author_facet Fadeev, Filip
Eremeev, Anton
Bashirov, Farid
Shevchenko, Roman
Izmailov, Andrei
Markosyan, Vage
Sokolov, Mikhail
Kalistratova, Julia
Khalitova, Anastasiia
Garifulin, Ravil
Islamov, Rustem
Lavrov, Igor
author_sort Fadeev, Filip
collection PubMed
description This study evaluates the effect of combined epidural electrical stimulation (EES) applied above (C5) and below (L2) the spinal cord injury (SCI) at T8–9 combined with motor training on the restoration of sensorimotor function in mini pigs. The motor evoked potentials (MEP) induced by EES applied at C5 and L2 levels were recorded in soleus muscles before and two weeks after SCI. EES treatment started two weeks after SCI and continued for 6 weeks led to improvement in multiple metrics, including behavioral, electrophysiological, and joint kinematics outcomes. In control animals after SCI a multiphasic M-response was observed during M/H-response testing, while animals received EES-enable training demonstrated the restoration of the M-response and H-reflex, although at a lower amplitude. The joint kinematic and assessment with Porcine Thoracic Injury Behavior scale (PTIBS) motor recovery scale demonstrated improvement in animals that received EES-enable training compared to animals with no treatment. The positive effect of two-level (cervical and lumbar) epidural electrical stimulation on functional restoration in mini pigs following spinal cord contusion injury in mini pigs could be related with facilitation of spinal circuitry at both levels and activation of multisegmental coordination. This approach can be taken as a basis for the future development of neuromodulation and neurorehabilitation therapy for patients with spinal cord injury.
format Online
Article
Text
id pubmed-7650717
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-76507172020-11-10 Combined Supra- and Sub-Lesional Epidural Electrical Stimulation for Restoration of the Motor Functions after Spinal Cord Injury in Mini Pigs Fadeev, Filip Eremeev, Anton Bashirov, Farid Shevchenko, Roman Izmailov, Andrei Markosyan, Vage Sokolov, Mikhail Kalistratova, Julia Khalitova, Anastasiia Garifulin, Ravil Islamov, Rustem Lavrov, Igor Brain Sci Article This study evaluates the effect of combined epidural electrical stimulation (EES) applied above (C5) and below (L2) the spinal cord injury (SCI) at T8–9 combined with motor training on the restoration of sensorimotor function in mini pigs. The motor evoked potentials (MEP) induced by EES applied at C5 and L2 levels were recorded in soleus muscles before and two weeks after SCI. EES treatment started two weeks after SCI and continued for 6 weeks led to improvement in multiple metrics, including behavioral, electrophysiological, and joint kinematics outcomes. In control animals after SCI a multiphasic M-response was observed during M/H-response testing, while animals received EES-enable training demonstrated the restoration of the M-response and H-reflex, although at a lower amplitude. The joint kinematic and assessment with Porcine Thoracic Injury Behavior scale (PTIBS) motor recovery scale demonstrated improvement in animals that received EES-enable training compared to animals with no treatment. The positive effect of two-level (cervical and lumbar) epidural electrical stimulation on functional restoration in mini pigs following spinal cord contusion injury in mini pigs could be related with facilitation of spinal circuitry at both levels and activation of multisegmental coordination. This approach can be taken as a basis for the future development of neuromodulation and neurorehabilitation therapy for patients with spinal cord injury. MDPI 2020-10-16 /pmc/articles/PMC7650717/ /pubmed/33081405 http://dx.doi.org/10.3390/brainsci10100744 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fadeev, Filip
Eremeev, Anton
Bashirov, Farid
Shevchenko, Roman
Izmailov, Andrei
Markosyan, Vage
Sokolov, Mikhail
Kalistratova, Julia
Khalitova, Anastasiia
Garifulin, Ravil
Islamov, Rustem
Lavrov, Igor
Combined Supra- and Sub-Lesional Epidural Electrical Stimulation for Restoration of the Motor Functions after Spinal Cord Injury in Mini Pigs
title Combined Supra- and Sub-Lesional Epidural Electrical Stimulation for Restoration of the Motor Functions after Spinal Cord Injury in Mini Pigs
title_full Combined Supra- and Sub-Lesional Epidural Electrical Stimulation for Restoration of the Motor Functions after Spinal Cord Injury in Mini Pigs
title_fullStr Combined Supra- and Sub-Lesional Epidural Electrical Stimulation for Restoration of the Motor Functions after Spinal Cord Injury in Mini Pigs
title_full_unstemmed Combined Supra- and Sub-Lesional Epidural Electrical Stimulation for Restoration of the Motor Functions after Spinal Cord Injury in Mini Pigs
title_short Combined Supra- and Sub-Lesional Epidural Electrical Stimulation for Restoration of the Motor Functions after Spinal Cord Injury in Mini Pigs
title_sort combined supra- and sub-lesional epidural electrical stimulation for restoration of the motor functions after spinal cord injury in mini pigs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7650717/
https://www.ncbi.nlm.nih.gov/pubmed/33081405
http://dx.doi.org/10.3390/brainsci10100744
work_keys_str_mv AT fadeevfilip combinedsupraandsublesionalepiduralelectricalstimulationforrestorationofthemotorfunctionsafterspinalcordinjuryinminipigs
AT eremeevanton combinedsupraandsublesionalepiduralelectricalstimulationforrestorationofthemotorfunctionsafterspinalcordinjuryinminipigs
AT bashirovfarid combinedsupraandsublesionalepiduralelectricalstimulationforrestorationofthemotorfunctionsafterspinalcordinjuryinminipigs
AT shevchenkoroman combinedsupraandsublesionalepiduralelectricalstimulationforrestorationofthemotorfunctionsafterspinalcordinjuryinminipigs
AT izmailovandrei combinedsupraandsublesionalepiduralelectricalstimulationforrestorationofthemotorfunctionsafterspinalcordinjuryinminipigs
AT markosyanvage combinedsupraandsublesionalepiduralelectricalstimulationforrestorationofthemotorfunctionsafterspinalcordinjuryinminipigs
AT sokolovmikhail combinedsupraandsublesionalepiduralelectricalstimulationforrestorationofthemotorfunctionsafterspinalcordinjuryinminipigs
AT kalistratovajulia combinedsupraandsublesionalepiduralelectricalstimulationforrestorationofthemotorfunctionsafterspinalcordinjuryinminipigs
AT khalitovaanastasiia combinedsupraandsublesionalepiduralelectricalstimulationforrestorationofthemotorfunctionsafterspinalcordinjuryinminipigs
AT garifulinravil combinedsupraandsublesionalepiduralelectricalstimulationforrestorationofthemotorfunctionsafterspinalcordinjuryinminipigs
AT islamovrustem combinedsupraandsublesionalepiduralelectricalstimulationforrestorationofthemotorfunctionsafterspinalcordinjuryinminipigs
AT lavrovigor combinedsupraandsublesionalepiduralelectricalstimulationforrestorationofthemotorfunctionsafterspinalcordinjuryinminipigs