Cargando…

Clinical Characteristics of Acute Hepatitis E and Their Correlation with HEV Genotype 3 Subtypes in Italy

Genotype 3 (GT3) is responsible for most European autochthonous hepatitis E virus (HEV) infections. This study analyzed circulating genotypes and GT3 subtypes in the Lazio region, Italy, between 2011 and 2019, as well as their pathogenic characteristics. Of the 64 evaluable HEV GT3 patient-derived s...

Descripción completa

Detalles Bibliográficos
Autores principales: Minosse, Claudia, Biliotti, Elisa, Lapa, Daniele, Rianda, Alessia, Marchili, Mauro, Luzzitelli, Ilaria, Capobianchi, Maria Rosaria, McPhee, Fiona, Garbuglia, Anna Rosa, D’Offizi, Gianpiero
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7650787/
https://www.ncbi.nlm.nih.gov/pubmed/33050666
http://dx.doi.org/10.3390/pathogens9100832
_version_ 1783607554176188416
author Minosse, Claudia
Biliotti, Elisa
Lapa, Daniele
Rianda, Alessia
Marchili, Mauro
Luzzitelli, Ilaria
Capobianchi, Maria Rosaria
McPhee, Fiona
Garbuglia, Anna Rosa
D’Offizi, Gianpiero
author_facet Minosse, Claudia
Biliotti, Elisa
Lapa, Daniele
Rianda, Alessia
Marchili, Mauro
Luzzitelli, Ilaria
Capobianchi, Maria Rosaria
McPhee, Fiona
Garbuglia, Anna Rosa
D’Offizi, Gianpiero
author_sort Minosse, Claudia
collection PubMed
description Genotype 3 (GT3) is responsible for most European autochthonous hepatitis E virus (HEV) infections. This study analyzed circulating genotypes and GT3 subtypes in the Lazio region, Italy, between 2011 and 2019, as well as their pathogenic characteristics. Of the 64 evaluable HEV GT3 patient-derived sequences, identified subtypes included GT3f (n = 36), GT3e (n = 15), GT3c (n = 9), GT3a (n = 1) and three unsubtyped GT3 sequences. GT3c strains were similar to Dutch sequences (96.8–98.1% identity), GT3e strains showed high similarity (96.8%) with a United Kingdom sequence, while the most related sequences to GT3f Italian strains were isolated in France, Belgium and Japan. One sequence was closely related to another Italian strain isolated in raw sewage in 2016. The liver functioning test median values for 56 evaluable GT3 patients were: alanine aminotransferase (ALT), 461 (range 52–4835 U/L); aspartate aminotransferase (AST), 659 (range 64–6588 U/L); and total bilirubin, 3.49 (range 0.4–33 mg/dL). The median HEV RNA viral load for 26 evaluable GT3 patients was 42,240 IU/mL (range 5680–895,490 IU/mL). Of the 37 GT3 patients with available clinical information, no correlation was observed between HEV clinical manifestations and GT3 subtype. HEV symptoms were comparable among GT3c/e/f patients across most analyzed categories except for epigastric pain, which occurred more frequently in patients with HEV GT3e (75%) than in patients with GT3c (50%) or GT3f (19%) (p = 0.01). Additionally, patients with HEV GT3c exhibited significantly higher median international normalized ratio (INR) than patients with GT3e and GT3f (p = 0.033). The severity of GT3 acute hepatitis E was not linked to HEV RNA viral load or to the GT3 subtype.
format Online
Article
Text
id pubmed-7650787
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-76507872020-11-10 Clinical Characteristics of Acute Hepatitis E and Their Correlation with HEV Genotype 3 Subtypes in Italy Minosse, Claudia Biliotti, Elisa Lapa, Daniele Rianda, Alessia Marchili, Mauro Luzzitelli, Ilaria Capobianchi, Maria Rosaria McPhee, Fiona Garbuglia, Anna Rosa D’Offizi, Gianpiero Pathogens Article Genotype 3 (GT3) is responsible for most European autochthonous hepatitis E virus (HEV) infections. This study analyzed circulating genotypes and GT3 subtypes in the Lazio region, Italy, between 2011 and 2019, as well as their pathogenic characteristics. Of the 64 evaluable HEV GT3 patient-derived sequences, identified subtypes included GT3f (n = 36), GT3e (n = 15), GT3c (n = 9), GT3a (n = 1) and three unsubtyped GT3 sequences. GT3c strains were similar to Dutch sequences (96.8–98.1% identity), GT3e strains showed high similarity (96.8%) with a United Kingdom sequence, while the most related sequences to GT3f Italian strains were isolated in France, Belgium and Japan. One sequence was closely related to another Italian strain isolated in raw sewage in 2016. The liver functioning test median values for 56 evaluable GT3 patients were: alanine aminotransferase (ALT), 461 (range 52–4835 U/L); aspartate aminotransferase (AST), 659 (range 64–6588 U/L); and total bilirubin, 3.49 (range 0.4–33 mg/dL). The median HEV RNA viral load for 26 evaluable GT3 patients was 42,240 IU/mL (range 5680–895,490 IU/mL). Of the 37 GT3 patients with available clinical information, no correlation was observed between HEV clinical manifestations and GT3 subtype. HEV symptoms were comparable among GT3c/e/f patients across most analyzed categories except for epigastric pain, which occurred more frequently in patients with HEV GT3e (75%) than in patients with GT3c (50%) or GT3f (19%) (p = 0.01). Additionally, patients with HEV GT3c exhibited significantly higher median international normalized ratio (INR) than patients with GT3e and GT3f (p = 0.033). The severity of GT3 acute hepatitis E was not linked to HEV RNA viral load or to the GT3 subtype. MDPI 2020-10-11 /pmc/articles/PMC7650787/ /pubmed/33050666 http://dx.doi.org/10.3390/pathogens9100832 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Minosse, Claudia
Biliotti, Elisa
Lapa, Daniele
Rianda, Alessia
Marchili, Mauro
Luzzitelli, Ilaria
Capobianchi, Maria Rosaria
McPhee, Fiona
Garbuglia, Anna Rosa
D’Offizi, Gianpiero
Clinical Characteristics of Acute Hepatitis E and Their Correlation with HEV Genotype 3 Subtypes in Italy
title Clinical Characteristics of Acute Hepatitis E and Their Correlation with HEV Genotype 3 Subtypes in Italy
title_full Clinical Characteristics of Acute Hepatitis E and Their Correlation with HEV Genotype 3 Subtypes in Italy
title_fullStr Clinical Characteristics of Acute Hepatitis E and Their Correlation with HEV Genotype 3 Subtypes in Italy
title_full_unstemmed Clinical Characteristics of Acute Hepatitis E and Their Correlation with HEV Genotype 3 Subtypes in Italy
title_short Clinical Characteristics of Acute Hepatitis E and Their Correlation with HEV Genotype 3 Subtypes in Italy
title_sort clinical characteristics of acute hepatitis e and their correlation with hev genotype 3 subtypes in italy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7650787/
https://www.ncbi.nlm.nih.gov/pubmed/33050666
http://dx.doi.org/10.3390/pathogens9100832
work_keys_str_mv AT minosseclaudia clinicalcharacteristicsofacutehepatitiseandtheircorrelationwithhevgenotype3subtypesinitaly
AT biliottielisa clinicalcharacteristicsofacutehepatitiseandtheircorrelationwithhevgenotype3subtypesinitaly
AT lapadaniele clinicalcharacteristicsofacutehepatitiseandtheircorrelationwithhevgenotype3subtypesinitaly
AT riandaalessia clinicalcharacteristicsofacutehepatitiseandtheircorrelationwithhevgenotype3subtypesinitaly
AT marchilimauro clinicalcharacteristicsofacutehepatitiseandtheircorrelationwithhevgenotype3subtypesinitaly
AT luzzitelliilaria clinicalcharacteristicsofacutehepatitiseandtheircorrelationwithhevgenotype3subtypesinitaly
AT capobianchimariarosaria clinicalcharacteristicsofacutehepatitiseandtheircorrelationwithhevgenotype3subtypesinitaly
AT mcpheefiona clinicalcharacteristicsofacutehepatitiseandtheircorrelationwithhevgenotype3subtypesinitaly
AT garbugliaannarosa clinicalcharacteristicsofacutehepatitiseandtheircorrelationwithhevgenotype3subtypesinitaly
AT doffizigianpiero clinicalcharacteristicsofacutehepatitiseandtheircorrelationwithhevgenotype3subtypesinitaly