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Experimental yellow fever virus infection in the squirrel monkey (Saimiri spp.) I: gross anatomical and histopathological findings in organs at necropsy

BACKGROUND: Non-human primates contribute to the spread of the yellow fever virus (YFV) and the establishment of transmission cycles in endemic areas. OBJECTIVE: To describe the severe histopathological aspects of YFV infection, 10 squirrel monkeys were infected with YFV and blood, brain, liver, kid...

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Autores principales: Ferreira, Milene Silveira, Júnior, Pedro Soares Bezerra, Cerqueira, Valíria Duarte, Rivero, Gabriela Riet Correa, Júnior, Carlos Alberto Oliveira, Castro, Paulo Henrique Gomes, da Silva, Gilmara Abreu, da Silva, Wellington Bandeira, Imbeloni, Aline Amaral, Sousa, Jorge Rodrigues, Araújo, Ana Paula Sousa, Silva, Franko de Arruda e, Tesh, Robert B, Quaresma, Juarez Antônio Simões, Vasconcelos, Pedro Fernando da Costa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Oswaldo Cruz, Ministério da Saúde 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7651848/
https://www.ncbi.nlm.nih.gov/pubmed/33174908
http://dx.doi.org/10.1590/0074-02760190501
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author Ferreira, Milene Silveira
Júnior, Pedro Soares Bezerra
Cerqueira, Valíria Duarte
Rivero, Gabriela Riet Correa
Júnior, Carlos Alberto Oliveira
Castro, Paulo Henrique Gomes
da Silva, Gilmara Abreu
da Silva, Wellington Bandeira
Imbeloni, Aline Amaral
Sousa, Jorge Rodrigues
Araújo, Ana Paula Sousa
Silva, Franko de Arruda e
Tesh, Robert B
Quaresma, Juarez Antônio Simões
Vasconcelos, Pedro Fernando da Costa
author_facet Ferreira, Milene Silveira
Júnior, Pedro Soares Bezerra
Cerqueira, Valíria Duarte
Rivero, Gabriela Riet Correa
Júnior, Carlos Alberto Oliveira
Castro, Paulo Henrique Gomes
da Silva, Gilmara Abreu
da Silva, Wellington Bandeira
Imbeloni, Aline Amaral
Sousa, Jorge Rodrigues
Araújo, Ana Paula Sousa
Silva, Franko de Arruda e
Tesh, Robert B
Quaresma, Juarez Antônio Simões
Vasconcelos, Pedro Fernando da Costa
author_sort Ferreira, Milene Silveira
collection PubMed
description BACKGROUND: Non-human primates contribute to the spread of the yellow fever virus (YFV) and the establishment of transmission cycles in endemic areas. OBJECTIVE: To describe the severe histopathological aspects of YFV infection, 10 squirrel monkeys were infected with YFV and blood, brain, liver, kidney, spleen, heart, lung, lymph node and stomach were collected at 1-7, 10, 20 and 30 days post-infection (dpi). METHODS: Histopathological analysis and detection of the genome and viral antigens and neutralising antibodies were performed by RT-PCR, immunohistochemistry and neutralisation test, respectively. FINDINGS: Only one animal died from the experimental infection. The genome and viral antigens were detected in all investigated organs (1-30 dpi) and the neutralising antibodies from seven to 30 dpi. The brain contained perivascular haemorrhage (6 dpi); in the liver, midzonal haemorrhage and lytic necrosis (6 dpi) were observed. The kidney had bleeding in the Bowman’s capsule and tubular necrosis (6 dpi). Pyknotic lymphocytes were observed in the spleen (1-20 dpi), the lung had haemorrhage (2-6 dpi), in the endocardium it contained nuclear pyknosis and necrosis (2-3 dpi) and the stomach contained blood in the lumen (6 dpi). MAIN FINDINGS: Squirrel monkeys reliably reproduced the responses observed in human cases of yellow fever and, therefore, constitute an excellent experimental model for studies on the pathophysiology of the disease.
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spelling pubmed-76518482020-11-17 Experimental yellow fever virus infection in the squirrel monkey (Saimiri spp.) I: gross anatomical and histopathological findings in organs at necropsy Ferreira, Milene Silveira Júnior, Pedro Soares Bezerra Cerqueira, Valíria Duarte Rivero, Gabriela Riet Correa Júnior, Carlos Alberto Oliveira Castro, Paulo Henrique Gomes da Silva, Gilmara Abreu da Silva, Wellington Bandeira Imbeloni, Aline Amaral Sousa, Jorge Rodrigues Araújo, Ana Paula Sousa Silva, Franko de Arruda e Tesh, Robert B Quaresma, Juarez Antônio Simões Vasconcelos, Pedro Fernando da Costa Mem Inst Oswaldo Cruz Original Article BACKGROUND: Non-human primates contribute to the spread of the yellow fever virus (YFV) and the establishment of transmission cycles in endemic areas. OBJECTIVE: To describe the severe histopathological aspects of YFV infection, 10 squirrel monkeys were infected with YFV and blood, brain, liver, kidney, spleen, heart, lung, lymph node and stomach were collected at 1-7, 10, 20 and 30 days post-infection (dpi). METHODS: Histopathological analysis and detection of the genome and viral antigens and neutralising antibodies were performed by RT-PCR, immunohistochemistry and neutralisation test, respectively. FINDINGS: Only one animal died from the experimental infection. The genome and viral antigens were detected in all investigated organs (1-30 dpi) and the neutralising antibodies from seven to 30 dpi. The brain contained perivascular haemorrhage (6 dpi); in the liver, midzonal haemorrhage and lytic necrosis (6 dpi) were observed. The kidney had bleeding in the Bowman’s capsule and tubular necrosis (6 dpi). Pyknotic lymphocytes were observed in the spleen (1-20 dpi), the lung had haemorrhage (2-6 dpi), in the endocardium it contained nuclear pyknosis and necrosis (2-3 dpi) and the stomach contained blood in the lumen (6 dpi). MAIN FINDINGS: Squirrel monkeys reliably reproduced the responses observed in human cases of yellow fever and, therefore, constitute an excellent experimental model for studies on the pathophysiology of the disease. Instituto Oswaldo Cruz, Ministério da Saúde 2020-11-09 /pmc/articles/PMC7651848/ /pubmed/33174908 http://dx.doi.org/10.1590/0074-02760190501 Text en https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License
spellingShingle Original Article
Ferreira, Milene Silveira
Júnior, Pedro Soares Bezerra
Cerqueira, Valíria Duarte
Rivero, Gabriela Riet Correa
Júnior, Carlos Alberto Oliveira
Castro, Paulo Henrique Gomes
da Silva, Gilmara Abreu
da Silva, Wellington Bandeira
Imbeloni, Aline Amaral
Sousa, Jorge Rodrigues
Araújo, Ana Paula Sousa
Silva, Franko de Arruda e
Tesh, Robert B
Quaresma, Juarez Antônio Simões
Vasconcelos, Pedro Fernando da Costa
Experimental yellow fever virus infection in the squirrel monkey (Saimiri spp.) I: gross anatomical and histopathological findings in organs at necropsy
title Experimental yellow fever virus infection in the squirrel monkey (Saimiri spp.) I: gross anatomical and histopathological findings in organs at necropsy
title_full Experimental yellow fever virus infection in the squirrel monkey (Saimiri spp.) I: gross anatomical and histopathological findings in organs at necropsy
title_fullStr Experimental yellow fever virus infection in the squirrel monkey (Saimiri spp.) I: gross anatomical and histopathological findings in organs at necropsy
title_full_unstemmed Experimental yellow fever virus infection in the squirrel monkey (Saimiri spp.) I: gross anatomical and histopathological findings in organs at necropsy
title_short Experimental yellow fever virus infection in the squirrel monkey (Saimiri spp.) I: gross anatomical and histopathological findings in organs at necropsy
title_sort experimental yellow fever virus infection in the squirrel monkey (saimiri spp.) i: gross anatomical and histopathological findings in organs at necropsy
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7651848/
https://www.ncbi.nlm.nih.gov/pubmed/33174908
http://dx.doi.org/10.1590/0074-02760190501
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