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MicroRNA-208a-3p promotes osteosarcoma progression via targeting PTEN

Osteosarcoma (OS) is a malignant bone tumor with a poor prognosis. Accumulated evidence has suggested that microRNAs (miRNAs/miRs) may function as either oncogenes or tumor suppressors, which are associated with tumorigenesis and the progression of different types of cancer. In the present study, th...

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Autores principales: Fu, Yutuo, Wang, Yan, Bi, Ke, Yang, Lei, Sun, Yi, Li, Boyuan, Liu, Zhenzhong, Zhang, Fulin, Li, Yuan, Feng, Chao, Bi, Zhenggang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7651880/
https://www.ncbi.nlm.nih.gov/pubmed/33178353
http://dx.doi.org/10.3892/etm.2020.9385
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author Fu, Yutuo
Wang, Yan
Bi, Ke
Yang, Lei
Sun, Yi
Li, Boyuan
Liu, Zhenzhong
Zhang, Fulin
Li, Yuan
Feng, Chao
Bi, Zhenggang
author_facet Fu, Yutuo
Wang, Yan
Bi, Ke
Yang, Lei
Sun, Yi
Li, Boyuan
Liu, Zhenzhong
Zhang, Fulin
Li, Yuan
Feng, Chao
Bi, Zhenggang
author_sort Fu, Yutuo
collection PubMed
description Osteosarcoma (OS) is a malignant bone tumor with a poor prognosis. Accumulated evidence has suggested that microRNAs (miRNAs/miRs) may function as either oncogenes or tumor suppressors, which are associated with tumorigenesis and the progression of different types of cancer. In the present study, the role of miR-208a-3p in OS was investigated. The expression levels of miR-208a-3p in OS tissues and cell lines were determined via reverse transcription-quantitative PCR (RT-qPCR). MTT and colony formation assays were performed to verify the proliferation rate of OS cells. In addition, the effects of miR-208a-3p on the migration and invasion of OS cells were revealed using wound-healing and Transwell assays, respectively. Furthermore, the association between miR-208a-3p and phosphatase and tensin homolog (PTEN) 3'-untranslated region was determined via luciferase reporter assays, western blot and RT-qPCR analysis. The results indicated that miR-208a-3p was upregulated in OS tissues and cell lines compared with adjacent normal tissues and human osteoblastic cells, respectively. miR-208a-3p overexpression promoted and miR-208a-3p knockdown inhibited OS cells proliferation and metastatic potential. Additionally, PTEN was validated as a direct target of miR-208a-3p and its expression was negatively associate with that of miR-208a-3p in OS cells. Taken together, these results may suggest that miR-208a-3p promoted OS cells proliferation and metastatic potential via targeting PTEN. Therefore, miR-208a-3p may be considered as a diagnostic biomarker for OS.
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spelling pubmed-76518802020-11-10 MicroRNA-208a-3p promotes osteosarcoma progression via targeting PTEN Fu, Yutuo Wang, Yan Bi, Ke Yang, Lei Sun, Yi Li, Boyuan Liu, Zhenzhong Zhang, Fulin Li, Yuan Feng, Chao Bi, Zhenggang Exp Ther Med Articles Osteosarcoma (OS) is a malignant bone tumor with a poor prognosis. Accumulated evidence has suggested that microRNAs (miRNAs/miRs) may function as either oncogenes or tumor suppressors, which are associated with tumorigenesis and the progression of different types of cancer. In the present study, the role of miR-208a-3p in OS was investigated. The expression levels of miR-208a-3p in OS tissues and cell lines were determined via reverse transcription-quantitative PCR (RT-qPCR). MTT and colony formation assays were performed to verify the proliferation rate of OS cells. In addition, the effects of miR-208a-3p on the migration and invasion of OS cells were revealed using wound-healing and Transwell assays, respectively. Furthermore, the association between miR-208a-3p and phosphatase and tensin homolog (PTEN) 3'-untranslated region was determined via luciferase reporter assays, western blot and RT-qPCR analysis. The results indicated that miR-208a-3p was upregulated in OS tissues and cell lines compared with adjacent normal tissues and human osteoblastic cells, respectively. miR-208a-3p overexpression promoted and miR-208a-3p knockdown inhibited OS cells proliferation and metastatic potential. Additionally, PTEN was validated as a direct target of miR-208a-3p and its expression was negatively associate with that of miR-208a-3p in OS cells. Taken together, these results may suggest that miR-208a-3p promoted OS cells proliferation and metastatic potential via targeting PTEN. Therefore, miR-208a-3p may be considered as a diagnostic biomarker for OS. D.A. Spandidos 2020-12 2020-10-23 /pmc/articles/PMC7651880/ /pubmed/33178353 http://dx.doi.org/10.3892/etm.2020.9385 Text en Copyright: © Fu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Fu, Yutuo
Wang, Yan
Bi, Ke
Yang, Lei
Sun, Yi
Li, Boyuan
Liu, Zhenzhong
Zhang, Fulin
Li, Yuan
Feng, Chao
Bi, Zhenggang
MicroRNA-208a-3p promotes osteosarcoma progression via targeting PTEN
title MicroRNA-208a-3p promotes osteosarcoma progression via targeting PTEN
title_full MicroRNA-208a-3p promotes osteosarcoma progression via targeting PTEN
title_fullStr MicroRNA-208a-3p promotes osteosarcoma progression via targeting PTEN
title_full_unstemmed MicroRNA-208a-3p promotes osteosarcoma progression via targeting PTEN
title_short MicroRNA-208a-3p promotes osteosarcoma progression via targeting PTEN
title_sort microrna-208a-3p promotes osteosarcoma progression via targeting pten
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7651880/
https://www.ncbi.nlm.nih.gov/pubmed/33178353
http://dx.doi.org/10.3892/etm.2020.9385
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