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Evaluation of pro-inflammatory cytokines in frail Tunisian older adults

The present study was undertaken to evaluate serum levels of pro-inflammatory cytokines in Tunisian older adults and to examine the relationships between inflammatory marker levels, geriatric, and biochemical parameters. A cross-sectional study was conducted in a population of Tunisian older adults...

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Autores principales: Hammami, Sonia, Ghzaiel, Imen, Hammouda, Souha, Sakly, Nabil, Hammami, Mohamed, Zarrouk, Amira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7652286/
https://www.ncbi.nlm.nih.gov/pubmed/33166358
http://dx.doi.org/10.1371/journal.pone.0242152
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author Hammami, Sonia
Ghzaiel, Imen
Hammouda, Souha
Sakly, Nabil
Hammami, Mohamed
Zarrouk, Amira
author_facet Hammami, Sonia
Ghzaiel, Imen
Hammouda, Souha
Sakly, Nabil
Hammami, Mohamed
Zarrouk, Amira
author_sort Hammami, Sonia
collection PubMed
description The present study was undertaken to evaluate serum levels of pro-inflammatory cytokines in Tunisian older adults and to examine the relationships between inflammatory marker levels, geriatric, and biochemical parameters. A cross-sectional study was conducted in a population of Tunisian older adults (N = 141, aged 65 and over). Patients were recruited from the Department of Internal Medicine, Fattouma Bourguiba University Hospital (Monastir, Tunisia) and from a nursing home (Sousse, Tunisia). Comprehensive geriatric assessment, history taking and examination including functional and nutritional assessment were done for each participant. Enzyme-linked immunosorbent assay (ELISA) test was used to measure serum cytokine (TNF-α, IL-8, IL-6) levels. The modified Short Emergency Geriatric Assessment score (SEGAm) were used to classify patients as 51 very-frail, 40 frail, and 50 non-frail. The age of the participants (80 men, 61 women) ranged from 65 to 97 years. Serum levels of TNF-α, IL-8 and C-reactive protein (CRP) were significantly higher in very-frail participants compared to frail and non-frail ones. However, no significant differences in IL-6 levels were detected among frailty groups. After adjustment for age, CRP and IL-8 levels remained significantly associated with frailty. Analysis of the receiver operating characteristic (ROC) curve corresponding to IL-8 showed an area under the curve of 0.7 (p = 0.003; 95% CI [0.58–0.81]) and a predictive threshold of 5.27 pg/ml. Positive correlations were found between frailty score, IL-6, and IL-8 levels. In addition, a significant positive correlation was observed between IL-8 levels and Timed Up and Go test results. However, a negative correlation was observed between Mini Nutritional Assessment Short-Form score, IL-6 and CRP levels, as well as between Activities of Daily Living score and serum levels of TNF-α, IL-6, and CRP. In conclusion, the key findings of this study collectively support a role of pro-inflammatory cytokines, TNF-α, CRP, and especially IL-8 in the development of frailty in older adults.
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spelling pubmed-76522862020-11-18 Evaluation of pro-inflammatory cytokines in frail Tunisian older adults Hammami, Sonia Ghzaiel, Imen Hammouda, Souha Sakly, Nabil Hammami, Mohamed Zarrouk, Amira PLoS One Research Article The present study was undertaken to evaluate serum levels of pro-inflammatory cytokines in Tunisian older adults and to examine the relationships between inflammatory marker levels, geriatric, and biochemical parameters. A cross-sectional study was conducted in a population of Tunisian older adults (N = 141, aged 65 and over). Patients were recruited from the Department of Internal Medicine, Fattouma Bourguiba University Hospital (Monastir, Tunisia) and from a nursing home (Sousse, Tunisia). Comprehensive geriatric assessment, history taking and examination including functional and nutritional assessment were done for each participant. Enzyme-linked immunosorbent assay (ELISA) test was used to measure serum cytokine (TNF-α, IL-8, IL-6) levels. The modified Short Emergency Geriatric Assessment score (SEGAm) were used to classify patients as 51 very-frail, 40 frail, and 50 non-frail. The age of the participants (80 men, 61 women) ranged from 65 to 97 years. Serum levels of TNF-α, IL-8 and C-reactive protein (CRP) were significantly higher in very-frail participants compared to frail and non-frail ones. However, no significant differences in IL-6 levels were detected among frailty groups. After adjustment for age, CRP and IL-8 levels remained significantly associated with frailty. Analysis of the receiver operating characteristic (ROC) curve corresponding to IL-8 showed an area under the curve of 0.7 (p = 0.003; 95% CI [0.58–0.81]) and a predictive threshold of 5.27 pg/ml. Positive correlations were found between frailty score, IL-6, and IL-8 levels. In addition, a significant positive correlation was observed between IL-8 levels and Timed Up and Go test results. However, a negative correlation was observed between Mini Nutritional Assessment Short-Form score, IL-6 and CRP levels, as well as between Activities of Daily Living score and serum levels of TNF-α, IL-6, and CRP. In conclusion, the key findings of this study collectively support a role of pro-inflammatory cytokines, TNF-α, CRP, and especially IL-8 in the development of frailty in older adults. Public Library of Science 2020-11-09 /pmc/articles/PMC7652286/ /pubmed/33166358 http://dx.doi.org/10.1371/journal.pone.0242152 Text en © 2020 Hammami et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Hammami, Sonia
Ghzaiel, Imen
Hammouda, Souha
Sakly, Nabil
Hammami, Mohamed
Zarrouk, Amira
Evaluation of pro-inflammatory cytokines in frail Tunisian older adults
title Evaluation of pro-inflammatory cytokines in frail Tunisian older adults
title_full Evaluation of pro-inflammatory cytokines in frail Tunisian older adults
title_fullStr Evaluation of pro-inflammatory cytokines in frail Tunisian older adults
title_full_unstemmed Evaluation of pro-inflammatory cytokines in frail Tunisian older adults
title_short Evaluation of pro-inflammatory cytokines in frail Tunisian older adults
title_sort evaluation of pro-inflammatory cytokines in frail tunisian older adults
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7652286/
https://www.ncbi.nlm.nih.gov/pubmed/33166358
http://dx.doi.org/10.1371/journal.pone.0242152
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