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Glomerular developmental delay and proteinuria in the preterm neonatal rabbit
Recent advances in neonatal care have improved the survival rate of those born premature. But prenatal conditions, premature birth and clinical interventions can lead to transient and permanent problems in these fragile patients. Premature birth (<36 gestational weeks) occurs during critical rena...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7652305/ https://www.ncbi.nlm.nih.gov/pubmed/33166318 http://dx.doi.org/10.1371/journal.pone.0241384 |
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author | de Winter, Derek Salaets, Thomas Gie, André Deprest, Jan Levtchenko, Elena Toelen, Jaan |
author_facet | de Winter, Derek Salaets, Thomas Gie, André Deprest, Jan Levtchenko, Elena Toelen, Jaan |
author_sort | de Winter, Derek |
collection | PubMed |
description | Recent advances in neonatal care have improved the survival rate of those born premature. But prenatal conditions, premature birth and clinical interventions can lead to transient and permanent problems in these fragile patients. Premature birth (<36 gestational weeks) occurs during critical renal development and maturation. Some consequences have been observed but the exact pathophysiology is still not entirely known. This experimental animal study aims to investigate the effect of premature birth on postnatal nephrogenesis in premature neonatal rabbits compared to term rabbits of the same corrected age. We analyzed renal morphology, glomerular maturity and functional parameters (proteinuria and protein/creatinine ratio) in three cohorts of rabbit pups: preterm (G28), preterm at day 7 of life (G28+7) and term at day 4 of life (G31+4). We found no significant differences in kidney volume and weight, and relative kidney volume between the cohorts. Nephrogenic zone width increased significantly over time when comparing G31 + 4 to G28. The renal corpuscle surface area, in the inner cortex and outer cortex, tended to decrease significantly after birth in both preterm and term groups. With regard to glomerular maturity, we found that the kidneys in the preterm cohorts were still in an immature state (presence of vesicles and capillary loop stage). Importantly, significant differences in proteinuria and protein/creatinine ratio were found. G28 + 7 showed increased proteinuria (p = 0.019) and an increased protein/creatinine ratio (p = 0.023) in comparison to G31 +4. In conclusion, these results suggest that the preterm rabbit kidney tends to linger in the immature glomerular stages and shows signs of a reduced renal functionality compared to the kidney born at term, which could in time lead to short- and long-term health consequences. |
format | Online Article Text |
id | pubmed-7652305 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-76523052020-11-18 Glomerular developmental delay and proteinuria in the preterm neonatal rabbit de Winter, Derek Salaets, Thomas Gie, André Deprest, Jan Levtchenko, Elena Toelen, Jaan PLoS One Research Article Recent advances in neonatal care have improved the survival rate of those born premature. But prenatal conditions, premature birth and clinical interventions can lead to transient and permanent problems in these fragile patients. Premature birth (<36 gestational weeks) occurs during critical renal development and maturation. Some consequences have been observed but the exact pathophysiology is still not entirely known. This experimental animal study aims to investigate the effect of premature birth on postnatal nephrogenesis in premature neonatal rabbits compared to term rabbits of the same corrected age. We analyzed renal morphology, glomerular maturity and functional parameters (proteinuria and protein/creatinine ratio) in three cohorts of rabbit pups: preterm (G28), preterm at day 7 of life (G28+7) and term at day 4 of life (G31+4). We found no significant differences in kidney volume and weight, and relative kidney volume between the cohorts. Nephrogenic zone width increased significantly over time when comparing G31 + 4 to G28. The renal corpuscle surface area, in the inner cortex and outer cortex, tended to decrease significantly after birth in both preterm and term groups. With regard to glomerular maturity, we found that the kidneys in the preterm cohorts were still in an immature state (presence of vesicles and capillary loop stage). Importantly, significant differences in proteinuria and protein/creatinine ratio were found. G28 + 7 showed increased proteinuria (p = 0.019) and an increased protein/creatinine ratio (p = 0.023) in comparison to G31 +4. In conclusion, these results suggest that the preterm rabbit kidney tends to linger in the immature glomerular stages and shows signs of a reduced renal functionality compared to the kidney born at term, which could in time lead to short- and long-term health consequences. Public Library of Science 2020-11-09 /pmc/articles/PMC7652305/ /pubmed/33166318 http://dx.doi.org/10.1371/journal.pone.0241384 Text en © 2020 de Winter et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article de Winter, Derek Salaets, Thomas Gie, André Deprest, Jan Levtchenko, Elena Toelen, Jaan Glomerular developmental delay and proteinuria in the preterm neonatal rabbit |
title | Glomerular developmental delay and proteinuria in the preterm neonatal rabbit |
title_full | Glomerular developmental delay and proteinuria in the preterm neonatal rabbit |
title_fullStr | Glomerular developmental delay and proteinuria in the preterm neonatal rabbit |
title_full_unstemmed | Glomerular developmental delay and proteinuria in the preterm neonatal rabbit |
title_short | Glomerular developmental delay and proteinuria in the preterm neonatal rabbit |
title_sort | glomerular developmental delay and proteinuria in the preterm neonatal rabbit |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7652305/ https://www.ncbi.nlm.nih.gov/pubmed/33166318 http://dx.doi.org/10.1371/journal.pone.0241384 |
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