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Glomerular developmental delay and proteinuria in the preterm neonatal rabbit

Recent advances in neonatal care have improved the survival rate of those born premature. But prenatal conditions, premature birth and clinical interventions can lead to transient and permanent problems in these fragile patients. Premature birth (<36 gestational weeks) occurs during critical rena...

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Autores principales: de Winter, Derek, Salaets, Thomas, Gie, André, Deprest, Jan, Levtchenko, Elena, Toelen, Jaan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7652305/
https://www.ncbi.nlm.nih.gov/pubmed/33166318
http://dx.doi.org/10.1371/journal.pone.0241384
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author de Winter, Derek
Salaets, Thomas
Gie, André
Deprest, Jan
Levtchenko, Elena
Toelen, Jaan
author_facet de Winter, Derek
Salaets, Thomas
Gie, André
Deprest, Jan
Levtchenko, Elena
Toelen, Jaan
author_sort de Winter, Derek
collection PubMed
description Recent advances in neonatal care have improved the survival rate of those born premature. But prenatal conditions, premature birth and clinical interventions can lead to transient and permanent problems in these fragile patients. Premature birth (<36 gestational weeks) occurs during critical renal development and maturation. Some consequences have been observed but the exact pathophysiology is still not entirely known. This experimental animal study aims to investigate the effect of premature birth on postnatal nephrogenesis in premature neonatal rabbits compared to term rabbits of the same corrected age. We analyzed renal morphology, glomerular maturity and functional parameters (proteinuria and protein/creatinine ratio) in three cohorts of rabbit pups: preterm (G28), preterm at day 7 of life (G28+7) and term at day 4 of life (G31+4). We found no significant differences in kidney volume and weight, and relative kidney volume between the cohorts. Nephrogenic zone width increased significantly over time when comparing G31 + 4 to G28. The renal corpuscle surface area, in the inner cortex and outer cortex, tended to decrease significantly after birth in both preterm and term groups. With regard to glomerular maturity, we found that the kidneys in the preterm cohorts were still in an immature state (presence of vesicles and capillary loop stage). Importantly, significant differences in proteinuria and protein/creatinine ratio were found. G28 + 7 showed increased proteinuria (p = 0.019) and an increased protein/creatinine ratio (p = 0.023) in comparison to G31 +4. In conclusion, these results suggest that the preterm rabbit kidney tends to linger in the immature glomerular stages and shows signs of a reduced renal functionality compared to the kidney born at term, which could in time lead to short- and long-term health consequences.
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spelling pubmed-76523052020-11-18 Glomerular developmental delay and proteinuria in the preterm neonatal rabbit de Winter, Derek Salaets, Thomas Gie, André Deprest, Jan Levtchenko, Elena Toelen, Jaan PLoS One Research Article Recent advances in neonatal care have improved the survival rate of those born premature. But prenatal conditions, premature birth and clinical interventions can lead to transient and permanent problems in these fragile patients. Premature birth (<36 gestational weeks) occurs during critical renal development and maturation. Some consequences have been observed but the exact pathophysiology is still not entirely known. This experimental animal study aims to investigate the effect of premature birth on postnatal nephrogenesis in premature neonatal rabbits compared to term rabbits of the same corrected age. We analyzed renal morphology, glomerular maturity and functional parameters (proteinuria and protein/creatinine ratio) in three cohorts of rabbit pups: preterm (G28), preterm at day 7 of life (G28+7) and term at day 4 of life (G31+4). We found no significant differences in kidney volume and weight, and relative kidney volume between the cohorts. Nephrogenic zone width increased significantly over time when comparing G31 + 4 to G28. The renal corpuscle surface area, in the inner cortex and outer cortex, tended to decrease significantly after birth in both preterm and term groups. With regard to glomerular maturity, we found that the kidneys in the preterm cohorts were still in an immature state (presence of vesicles and capillary loop stage). Importantly, significant differences in proteinuria and protein/creatinine ratio were found. G28 + 7 showed increased proteinuria (p = 0.019) and an increased protein/creatinine ratio (p = 0.023) in comparison to G31 +4. In conclusion, these results suggest that the preterm rabbit kidney tends to linger in the immature glomerular stages and shows signs of a reduced renal functionality compared to the kidney born at term, which could in time lead to short- and long-term health consequences. Public Library of Science 2020-11-09 /pmc/articles/PMC7652305/ /pubmed/33166318 http://dx.doi.org/10.1371/journal.pone.0241384 Text en © 2020 de Winter et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
de Winter, Derek
Salaets, Thomas
Gie, André
Deprest, Jan
Levtchenko, Elena
Toelen, Jaan
Glomerular developmental delay and proteinuria in the preterm neonatal rabbit
title Glomerular developmental delay and proteinuria in the preterm neonatal rabbit
title_full Glomerular developmental delay and proteinuria in the preterm neonatal rabbit
title_fullStr Glomerular developmental delay and proteinuria in the preterm neonatal rabbit
title_full_unstemmed Glomerular developmental delay and proteinuria in the preterm neonatal rabbit
title_short Glomerular developmental delay and proteinuria in the preterm neonatal rabbit
title_sort glomerular developmental delay and proteinuria in the preterm neonatal rabbit
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7652305/
https://www.ncbi.nlm.nih.gov/pubmed/33166318
http://dx.doi.org/10.1371/journal.pone.0241384
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