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Need for Speed: Examining Protein Behavior during CryoEM Grid Preparation at Different Timescales

A host of new technologies are under development to improve the quality and reproducibility of cryoelectron microscopy (cryoEM) grid preparation. Here we have systematically investigated the preparation of three macromolecular complexes using three different vitrification devices (Vitrobot, chameleo...

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Detalles Bibliográficos
Autores principales: Klebl, David P., Gravett, Molly S.C., Kontziampasis, Dimitrios, Wright, David J., Bon, Robin S., Monteiro, Diana C.F., Trebbin, Martin, Sobott, Frank, White, Howard D., Darrow, Michele C., Thompson, Rebecca F., Muench, Stephen P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7652391/
https://www.ncbi.nlm.nih.gov/pubmed/32814033
http://dx.doi.org/10.1016/j.str.2020.07.018