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A single mutation in Crimean-Congo hemorrhagic fever virus discovered in ticks impairs infectivity in human cells

Crimean-Congo hemorrhagic fever (CCHF) is the most widely distributed tick-borne viral infection in the world. Strikingly, reported mortality rates for CCHF are extremely variable, ranging from 5% to 80% (Whitehouse, 2004). CCHF virus (CCHFV, Nairoviridae) exhibits extensive genomic sequence diversi...

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Autores principales: Hua, Brian L, Scholte, Florine EM, Ohlendorf, Valerie, Kopp, Anne, Marklewitz, Marco, Drosten, Christian, Nichol, Stuart T, Spiropoulou, Christina, Junglen, Sandra, Bergeron, Éric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7652417/
https://www.ncbi.nlm.nih.gov/pubmed/33084573
http://dx.doi.org/10.7554/eLife.50999
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author Hua, Brian L
Scholte, Florine EM
Ohlendorf, Valerie
Kopp, Anne
Marklewitz, Marco
Drosten, Christian
Nichol, Stuart T
Spiropoulou, Christina
Junglen, Sandra
Bergeron, Éric
author_facet Hua, Brian L
Scholte, Florine EM
Ohlendorf, Valerie
Kopp, Anne
Marklewitz, Marco
Drosten, Christian
Nichol, Stuart T
Spiropoulou, Christina
Junglen, Sandra
Bergeron, Éric
author_sort Hua, Brian L
collection PubMed
description Crimean-Congo hemorrhagic fever (CCHF) is the most widely distributed tick-borne viral infection in the world. Strikingly, reported mortality rates for CCHF are extremely variable, ranging from 5% to 80% (Whitehouse, 2004). CCHF virus (CCHFV, Nairoviridae) exhibits extensive genomic sequence diversity across strains (Deyde et al., 2006; Sherifi et al., 2014). It is currently unknown if genomic diversity is a factor contributing to variation in its pathogenicity. We obtained complete genome sequences of CCHFV directly from the tick reservoir. These new strains belong to a solitary lineage named Europe 2 that is circumstantially reputed to be less pathogenic than the epidemic strains from Europe 1 lineage. We identified a single tick-specific amino acid variant in the viral glycoprotein region that dramatically reduces its fusion activity in human cells, providing evidence that a glycoprotein precursor variant, present in ticks, has severely impaired function in human cells.
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spelling pubmed-76524172020-11-12 A single mutation in Crimean-Congo hemorrhagic fever virus discovered in ticks impairs infectivity in human cells Hua, Brian L Scholte, Florine EM Ohlendorf, Valerie Kopp, Anne Marklewitz, Marco Drosten, Christian Nichol, Stuart T Spiropoulou, Christina Junglen, Sandra Bergeron, Éric eLife Microbiology and Infectious Disease Crimean-Congo hemorrhagic fever (CCHF) is the most widely distributed tick-borne viral infection in the world. Strikingly, reported mortality rates for CCHF are extremely variable, ranging from 5% to 80% (Whitehouse, 2004). CCHF virus (CCHFV, Nairoviridae) exhibits extensive genomic sequence diversity across strains (Deyde et al., 2006; Sherifi et al., 2014). It is currently unknown if genomic diversity is a factor contributing to variation in its pathogenicity. We obtained complete genome sequences of CCHFV directly from the tick reservoir. These new strains belong to a solitary lineage named Europe 2 that is circumstantially reputed to be less pathogenic than the epidemic strains from Europe 1 lineage. We identified a single tick-specific amino acid variant in the viral glycoprotein region that dramatically reduces its fusion activity in human cells, providing evidence that a glycoprotein precursor variant, present in ticks, has severely impaired function in human cells. eLife Sciences Publications, Ltd 2020-10-21 /pmc/articles/PMC7652417/ /pubmed/33084573 http://dx.doi.org/10.7554/eLife.50999 Text en http://creativecommons.org/publicdomain/zero/1.0/ http://creativecommons.org/publicdomain/zero/1.0/This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication (http://creativecommons.org/publicdomain/zero/1.0/) .
spellingShingle Microbiology and Infectious Disease
Hua, Brian L
Scholte, Florine EM
Ohlendorf, Valerie
Kopp, Anne
Marklewitz, Marco
Drosten, Christian
Nichol, Stuart T
Spiropoulou, Christina
Junglen, Sandra
Bergeron, Éric
A single mutation in Crimean-Congo hemorrhagic fever virus discovered in ticks impairs infectivity in human cells
title A single mutation in Crimean-Congo hemorrhagic fever virus discovered in ticks impairs infectivity in human cells
title_full A single mutation in Crimean-Congo hemorrhagic fever virus discovered in ticks impairs infectivity in human cells
title_fullStr A single mutation in Crimean-Congo hemorrhagic fever virus discovered in ticks impairs infectivity in human cells
title_full_unstemmed A single mutation in Crimean-Congo hemorrhagic fever virus discovered in ticks impairs infectivity in human cells
title_short A single mutation in Crimean-Congo hemorrhagic fever virus discovered in ticks impairs infectivity in human cells
title_sort single mutation in crimean-congo hemorrhagic fever virus discovered in ticks impairs infectivity in human cells
topic Microbiology and Infectious Disease
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7652417/
https://www.ncbi.nlm.nih.gov/pubmed/33084573
http://dx.doi.org/10.7554/eLife.50999
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