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A single mutation in Crimean-Congo hemorrhagic fever virus discovered in ticks impairs infectivity in human cells
Crimean-Congo hemorrhagic fever (CCHF) is the most widely distributed tick-borne viral infection in the world. Strikingly, reported mortality rates for CCHF are extremely variable, ranging from 5% to 80% (Whitehouse, 2004). CCHF virus (CCHFV, Nairoviridae) exhibits extensive genomic sequence diversi...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7652417/ https://www.ncbi.nlm.nih.gov/pubmed/33084573 http://dx.doi.org/10.7554/eLife.50999 |
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author | Hua, Brian L Scholte, Florine EM Ohlendorf, Valerie Kopp, Anne Marklewitz, Marco Drosten, Christian Nichol, Stuart T Spiropoulou, Christina Junglen, Sandra Bergeron, Éric |
author_facet | Hua, Brian L Scholte, Florine EM Ohlendorf, Valerie Kopp, Anne Marklewitz, Marco Drosten, Christian Nichol, Stuart T Spiropoulou, Christina Junglen, Sandra Bergeron, Éric |
author_sort | Hua, Brian L |
collection | PubMed |
description | Crimean-Congo hemorrhagic fever (CCHF) is the most widely distributed tick-borne viral infection in the world. Strikingly, reported mortality rates for CCHF are extremely variable, ranging from 5% to 80% (Whitehouse, 2004). CCHF virus (CCHFV, Nairoviridae) exhibits extensive genomic sequence diversity across strains (Deyde et al., 2006; Sherifi et al., 2014). It is currently unknown if genomic diversity is a factor contributing to variation in its pathogenicity. We obtained complete genome sequences of CCHFV directly from the tick reservoir. These new strains belong to a solitary lineage named Europe 2 that is circumstantially reputed to be less pathogenic than the epidemic strains from Europe 1 lineage. We identified a single tick-specific amino acid variant in the viral glycoprotein region that dramatically reduces its fusion activity in human cells, providing evidence that a glycoprotein precursor variant, present in ticks, has severely impaired function in human cells. |
format | Online Article Text |
id | pubmed-7652417 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-76524172020-11-12 A single mutation in Crimean-Congo hemorrhagic fever virus discovered in ticks impairs infectivity in human cells Hua, Brian L Scholte, Florine EM Ohlendorf, Valerie Kopp, Anne Marklewitz, Marco Drosten, Christian Nichol, Stuart T Spiropoulou, Christina Junglen, Sandra Bergeron, Éric eLife Microbiology and Infectious Disease Crimean-Congo hemorrhagic fever (CCHF) is the most widely distributed tick-borne viral infection in the world. Strikingly, reported mortality rates for CCHF are extremely variable, ranging from 5% to 80% (Whitehouse, 2004). CCHF virus (CCHFV, Nairoviridae) exhibits extensive genomic sequence diversity across strains (Deyde et al., 2006; Sherifi et al., 2014). It is currently unknown if genomic diversity is a factor contributing to variation in its pathogenicity. We obtained complete genome sequences of CCHFV directly from the tick reservoir. These new strains belong to a solitary lineage named Europe 2 that is circumstantially reputed to be less pathogenic than the epidemic strains from Europe 1 lineage. We identified a single tick-specific amino acid variant in the viral glycoprotein region that dramatically reduces its fusion activity in human cells, providing evidence that a glycoprotein precursor variant, present in ticks, has severely impaired function in human cells. eLife Sciences Publications, Ltd 2020-10-21 /pmc/articles/PMC7652417/ /pubmed/33084573 http://dx.doi.org/10.7554/eLife.50999 Text en http://creativecommons.org/publicdomain/zero/1.0/ http://creativecommons.org/publicdomain/zero/1.0/This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication (http://creativecommons.org/publicdomain/zero/1.0/) . |
spellingShingle | Microbiology and Infectious Disease Hua, Brian L Scholte, Florine EM Ohlendorf, Valerie Kopp, Anne Marklewitz, Marco Drosten, Christian Nichol, Stuart T Spiropoulou, Christina Junglen, Sandra Bergeron, Éric A single mutation in Crimean-Congo hemorrhagic fever virus discovered in ticks impairs infectivity in human cells |
title | A single mutation in Crimean-Congo hemorrhagic fever virus discovered in ticks impairs infectivity in human cells |
title_full | A single mutation in Crimean-Congo hemorrhagic fever virus discovered in ticks impairs infectivity in human cells |
title_fullStr | A single mutation in Crimean-Congo hemorrhagic fever virus discovered in ticks impairs infectivity in human cells |
title_full_unstemmed | A single mutation in Crimean-Congo hemorrhagic fever virus discovered in ticks impairs infectivity in human cells |
title_short | A single mutation in Crimean-Congo hemorrhagic fever virus discovered in ticks impairs infectivity in human cells |
title_sort | single mutation in crimean-congo hemorrhagic fever virus discovered in ticks impairs infectivity in human cells |
topic | Microbiology and Infectious Disease |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7652417/ https://www.ncbi.nlm.nih.gov/pubmed/33084573 http://dx.doi.org/10.7554/eLife.50999 |
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