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Roles for the RNA polymerase III regulator MAFR-1 in regulating sperm quality in Caenorhabditis elegans
The negative regulator of RNA polymerase (pol) III mafr-1 has been shown to affect RNA pol III transcript abundance, lipid biosynthesis and storage, progeny output, and lifespan. We deleted mafr-1 from the Caenorhabditis elegans genome and found that animals lacking mafr-1 replicated many phenotypes...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7652826/ https://www.ncbi.nlm.nih.gov/pubmed/33168938 http://dx.doi.org/10.1038/s41598-020-76423-5 |
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author | Hammerquist, Amy M. Curran, Sean P. |
author_facet | Hammerquist, Amy M. Curran, Sean P. |
author_sort | Hammerquist, Amy M. |
collection | PubMed |
description | The negative regulator of RNA polymerase (pol) III mafr-1 has been shown to affect RNA pol III transcript abundance, lipid biosynthesis and storage, progeny output, and lifespan. We deleted mafr-1 from the Caenorhabditis elegans genome and found that animals lacking mafr-1 replicated many phenotypes from previous RNAi-based studies and discovered a new sperm-specific role. Utilizing a yeast two-hybrid assay, we discovered several novel interactors of MAFR-1 that are expressed in a sperm- and germline-enriched manner. In support of a role for MAFR-1 in the male germline, we found mafr-1 null males have smaller spermatids that are less capable in competition for fertilization; a phenotype that was dependent on RNA pol III activity. Restoration of MAFR-1 expression specifically in the germline rescued the spermatid-related phenotypes, suggesting a cell autonomous role for MAFR-1 in nematode male fertility. Based on the high degree of conservation of Maf1 activity across species, our study may inform similar roles for Maf1 and RNA pol III in mammalian male fertility. |
format | Online Article Text |
id | pubmed-7652826 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-76528262020-11-12 Roles for the RNA polymerase III regulator MAFR-1 in regulating sperm quality in Caenorhabditis elegans Hammerquist, Amy M. Curran, Sean P. Sci Rep Article The negative regulator of RNA polymerase (pol) III mafr-1 has been shown to affect RNA pol III transcript abundance, lipid biosynthesis and storage, progeny output, and lifespan. We deleted mafr-1 from the Caenorhabditis elegans genome and found that animals lacking mafr-1 replicated many phenotypes from previous RNAi-based studies and discovered a new sperm-specific role. Utilizing a yeast two-hybrid assay, we discovered several novel interactors of MAFR-1 that are expressed in a sperm- and germline-enriched manner. In support of a role for MAFR-1 in the male germline, we found mafr-1 null males have smaller spermatids that are less capable in competition for fertilization; a phenotype that was dependent on RNA pol III activity. Restoration of MAFR-1 expression specifically in the germline rescued the spermatid-related phenotypes, suggesting a cell autonomous role for MAFR-1 in nematode male fertility. Based on the high degree of conservation of Maf1 activity across species, our study may inform similar roles for Maf1 and RNA pol III in mammalian male fertility. Nature Publishing Group UK 2020-11-09 /pmc/articles/PMC7652826/ /pubmed/33168938 http://dx.doi.org/10.1038/s41598-020-76423-5 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Hammerquist, Amy M. Curran, Sean P. Roles for the RNA polymerase III regulator MAFR-1 in regulating sperm quality in Caenorhabditis elegans |
title | Roles for the RNA polymerase III regulator MAFR-1 in regulating sperm quality in Caenorhabditis elegans |
title_full | Roles for the RNA polymerase III regulator MAFR-1 in regulating sperm quality in Caenorhabditis elegans |
title_fullStr | Roles for the RNA polymerase III regulator MAFR-1 in regulating sperm quality in Caenorhabditis elegans |
title_full_unstemmed | Roles for the RNA polymerase III regulator MAFR-1 in regulating sperm quality in Caenorhabditis elegans |
title_short | Roles for the RNA polymerase III regulator MAFR-1 in regulating sperm quality in Caenorhabditis elegans |
title_sort | roles for the rna polymerase iii regulator mafr-1 in regulating sperm quality in caenorhabditis elegans |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7652826/ https://www.ncbi.nlm.nih.gov/pubmed/33168938 http://dx.doi.org/10.1038/s41598-020-76423-5 |
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