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Bacterial etiology of sputum from tuberculosis suspected patients and antibiogram of the isolates

OBJECTIVE: The current study aims to explore the bacteriology of sputum of tuberculosis (TB) suspected patients. A cross-sectional study was carried out in the sputum samples of 150 TB suspected patients visiting District Public Health Office, Bharatpur, Nepal. The samples were subjected to cultural...

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Autores principales: Regmi, Ramesh Sharma, Khadka, Sujan, Sapkota, Sanjeep, Adhikari, Sanjib, Dhakal, Khuma Kumari, Dhakal, Bishnu, Lamsal, Bandana, Kafle, Sarad Chandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7654065/
https://www.ncbi.nlm.nih.gov/pubmed/33172496
http://dx.doi.org/10.1186/s13104-020-05369-8
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author Regmi, Ramesh Sharma
Khadka, Sujan
Sapkota, Sanjeep
Adhikari, Sanjib
Dhakal, Khuma Kumari
Dhakal, Bishnu
Lamsal, Bandana
Kafle, Sarad Chandra
author_facet Regmi, Ramesh Sharma
Khadka, Sujan
Sapkota, Sanjeep
Adhikari, Sanjib
Dhakal, Khuma Kumari
Dhakal, Bishnu
Lamsal, Bandana
Kafle, Sarad Chandra
author_sort Regmi, Ramesh Sharma
collection PubMed
description OBJECTIVE: The current study aims to explore the bacteriology of sputum of tuberculosis (TB) suspected patients. A cross-sectional study was carried out in the sputum samples of 150 TB suspected patients visiting District Public Health Office, Bharatpur, Nepal. The samples were subjected to cultural, microscopic and biochemical analyses for the identification of the isolates. In addition, antibiotic susceptibility tests were carried out with a special focus on ESBL and MBL production following Clinical and Laboratory Standard Institute guidelines. RESULTS: Bacterial growth was recovered in 47% (71/150) of the TB suspected patients of which 12.66% (19/150) had pulmonary TB infection. Streptococcus spp. (9%) and Pseudomonas aeruginosa (9%) were the most frequently isolated bacteria. Enterobacteriaceae accounted for 35% of the total isolates. Occurrence of bacterial pathogens was more in males (69%) than in females (31%).The incidence of bacterial pathogen was seen associated with gender of the patients and with the TB infection (p < 0.05) but independent with age of the patients and HIV infection (p > 0.05). Tetracycline was effective against Streptococcus spp. whereas gentamicin was effective against Bacillus species. Imipenem and co-trimoxazole were effective drugs for Gram-negative isolates. Among 83 isolates, 35 were multi-drug resistant, 9 were ESBL producers and 4 were MBL producers.
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spelling pubmed-76540652020-11-10 Bacterial etiology of sputum from tuberculosis suspected patients and antibiogram of the isolates Regmi, Ramesh Sharma Khadka, Sujan Sapkota, Sanjeep Adhikari, Sanjib Dhakal, Khuma Kumari Dhakal, Bishnu Lamsal, Bandana Kafle, Sarad Chandra BMC Res Notes Research Note OBJECTIVE: The current study aims to explore the bacteriology of sputum of tuberculosis (TB) suspected patients. A cross-sectional study was carried out in the sputum samples of 150 TB suspected patients visiting District Public Health Office, Bharatpur, Nepal. The samples were subjected to cultural, microscopic and biochemical analyses for the identification of the isolates. In addition, antibiotic susceptibility tests were carried out with a special focus on ESBL and MBL production following Clinical and Laboratory Standard Institute guidelines. RESULTS: Bacterial growth was recovered in 47% (71/150) of the TB suspected patients of which 12.66% (19/150) had pulmonary TB infection. Streptococcus spp. (9%) and Pseudomonas aeruginosa (9%) were the most frequently isolated bacteria. Enterobacteriaceae accounted for 35% of the total isolates. Occurrence of bacterial pathogens was more in males (69%) than in females (31%).The incidence of bacterial pathogen was seen associated with gender of the patients and with the TB infection (p < 0.05) but independent with age of the patients and HIV infection (p > 0.05). Tetracycline was effective against Streptococcus spp. whereas gentamicin was effective against Bacillus species. Imipenem and co-trimoxazole were effective drugs for Gram-negative isolates. Among 83 isolates, 35 were multi-drug resistant, 9 were ESBL producers and 4 were MBL producers. BioMed Central 2020-11-10 /pmc/articles/PMC7654065/ /pubmed/33172496 http://dx.doi.org/10.1186/s13104-020-05369-8 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Note
Regmi, Ramesh Sharma
Khadka, Sujan
Sapkota, Sanjeep
Adhikari, Sanjib
Dhakal, Khuma Kumari
Dhakal, Bishnu
Lamsal, Bandana
Kafle, Sarad Chandra
Bacterial etiology of sputum from tuberculosis suspected patients and antibiogram of the isolates
title Bacterial etiology of sputum from tuberculosis suspected patients and antibiogram of the isolates
title_full Bacterial etiology of sputum from tuberculosis suspected patients and antibiogram of the isolates
title_fullStr Bacterial etiology of sputum from tuberculosis suspected patients and antibiogram of the isolates
title_full_unstemmed Bacterial etiology of sputum from tuberculosis suspected patients and antibiogram of the isolates
title_short Bacterial etiology of sputum from tuberculosis suspected patients and antibiogram of the isolates
title_sort bacterial etiology of sputum from tuberculosis suspected patients and antibiogram of the isolates
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7654065/
https://www.ncbi.nlm.nih.gov/pubmed/33172496
http://dx.doi.org/10.1186/s13104-020-05369-8
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