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Fine mapping of the QTL cqSPDA2 for chlorophyll content in Brassica napus L.

BACKGROUND: Chlorophyll is the most important factor enabling plants to absorb, transfer and transform light energy and plays an important role in yield formation. Brassica napus is one of the most important oil crops. Breeding Brassica napus for high light efficiency by improving photosynthetic eff...

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Autores principales: Ye, Jingxiu, Liu, Haidong, Zhao, Zhi, Xu, Liang, Li, Kaixiang, Du, Dezhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7654151/
https://www.ncbi.nlm.nih.gov/pubmed/33167895
http://dx.doi.org/10.1186/s12870-020-02710-y
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author Ye, Jingxiu
Liu, Haidong
Zhao, Zhi
Xu, Liang
Li, Kaixiang
Du, Dezhi
author_facet Ye, Jingxiu
Liu, Haidong
Zhao, Zhi
Xu, Liang
Li, Kaixiang
Du, Dezhi
author_sort Ye, Jingxiu
collection PubMed
description BACKGROUND: Chlorophyll is the most important factor enabling plants to absorb, transfer and transform light energy and plays an important role in yield formation. Brassica napus is one of the most important oil crops. Breeding Brassica napus for high light efficiency by improving photosynthetic efficiency has considerable social and economic value. In Brassica napus, there have been studies of the initial location of chlorophyll in seed embryos and pericarps, but there are few reports on the fine mapping of chlorophyll QTLs. We constructed near-isogenic lines (NIL), fine-mapped a chlorophyll locus, and evaluated the effect of this dominant locus on agronomic traits. RESULTS: The cqSPDA2 locus was mapped to an interval of 21.87–22.91 Mb on the chromosome A02 of Brassica napus using doubled haploid (DH) lines. To fine-map cqSPDA2, we built NIL and designed Indel primers covering the mapping interval. The 469 individuals in the BC(3)F(2) population were analyzed using these indel primers. Among these indel primers, 15 could narrow the mapping interval to 188 kb between Indel3 and Indel15. Next, 16 indel primers and 19 SSR primers were designed within the new narrower mapping interval, and 5 of the primer-amplified fragments were found to be polymorphic and tightly linked to the cqSPDA2 locus in the BC(4)F(2) population. The mapping interval was narrowed to 152 kb on A02 between SSR2 and Indel15. By gene expression analysis, we found three annotated genes in the mapping interval, including BnaA02g30260D, BnaA02g30290D and BnaA02g30310D, which may be responsible for chlorophyll synthesis. CONCLUSIONS: The locus cqSPDA2, a dominant QTL for chlorophyll content in Brassica napus, was fine-mapped to a 21.89–22.04 Mb interval on A02(.) Three annotated genes (BnaA02g30260D, BnaA02g30290D and BnaA02g30310D) that may be responsible for chlorophyll synthesis were found. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-020-02710-y.
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spelling pubmed-76541512020-11-10 Fine mapping of the QTL cqSPDA2 for chlorophyll content in Brassica napus L. Ye, Jingxiu Liu, Haidong Zhao, Zhi Xu, Liang Li, Kaixiang Du, Dezhi BMC Plant Biol Research Article BACKGROUND: Chlorophyll is the most important factor enabling plants to absorb, transfer and transform light energy and plays an important role in yield formation. Brassica napus is one of the most important oil crops. Breeding Brassica napus for high light efficiency by improving photosynthetic efficiency has considerable social and economic value. In Brassica napus, there have been studies of the initial location of chlorophyll in seed embryos and pericarps, but there are few reports on the fine mapping of chlorophyll QTLs. We constructed near-isogenic lines (NIL), fine-mapped a chlorophyll locus, and evaluated the effect of this dominant locus on agronomic traits. RESULTS: The cqSPDA2 locus was mapped to an interval of 21.87–22.91 Mb on the chromosome A02 of Brassica napus using doubled haploid (DH) lines. To fine-map cqSPDA2, we built NIL and designed Indel primers covering the mapping interval. The 469 individuals in the BC(3)F(2) population were analyzed using these indel primers. Among these indel primers, 15 could narrow the mapping interval to 188 kb between Indel3 and Indel15. Next, 16 indel primers and 19 SSR primers were designed within the new narrower mapping interval, and 5 of the primer-amplified fragments were found to be polymorphic and tightly linked to the cqSPDA2 locus in the BC(4)F(2) population. The mapping interval was narrowed to 152 kb on A02 between SSR2 and Indel15. By gene expression analysis, we found three annotated genes in the mapping interval, including BnaA02g30260D, BnaA02g30290D and BnaA02g30310D, which may be responsible for chlorophyll synthesis. CONCLUSIONS: The locus cqSPDA2, a dominant QTL for chlorophyll content in Brassica napus, was fine-mapped to a 21.89–22.04 Mb interval on A02(.) Three annotated genes (BnaA02g30260D, BnaA02g30290D and BnaA02g30310D) that may be responsible for chlorophyll synthesis were found. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-020-02710-y. BioMed Central 2020-11-09 /pmc/articles/PMC7654151/ /pubmed/33167895 http://dx.doi.org/10.1186/s12870-020-02710-y Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Ye, Jingxiu
Liu, Haidong
Zhao, Zhi
Xu, Liang
Li, Kaixiang
Du, Dezhi
Fine mapping of the QTL cqSPDA2 for chlorophyll content in Brassica napus L.
title Fine mapping of the QTL cqSPDA2 for chlorophyll content in Brassica napus L.
title_full Fine mapping of the QTL cqSPDA2 for chlorophyll content in Brassica napus L.
title_fullStr Fine mapping of the QTL cqSPDA2 for chlorophyll content in Brassica napus L.
title_full_unstemmed Fine mapping of the QTL cqSPDA2 for chlorophyll content in Brassica napus L.
title_short Fine mapping of the QTL cqSPDA2 for chlorophyll content in Brassica napus L.
title_sort fine mapping of the qtl cqspda2 for chlorophyll content in brassica napus l.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7654151/
https://www.ncbi.nlm.nih.gov/pubmed/33167895
http://dx.doi.org/10.1186/s12870-020-02710-y
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