The Isolation and Molecular Characterization of an Astrovirus From “Yellow” Chickens, China

Chicken astrovirus (CAstV) infection is strongly associated with kidney disease, gout, “white chicks” hatchery disease, and runting and stunting syndrome (RSS). In the present study, 82.5% of 154 clinical samples from different provinces in China were positive for CAstV by RT-PCR. One CAstV isolate,...

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Autores principales: Zhao, Wei, Wu, Zongyi, Yao, Yongxiu, Qin, Aijian, Qian, Kun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Veterinary Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7655128/
https://www.ncbi.nlm.nih.gov/pubmed/33195598
http://dx.doi.org/10.3389/fvets.2020.581862
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author Zhao, Wei
Wu, Zongyi
Yao, Yongxiu
Qin, Aijian
Qian, Kun
author_facet Zhao, Wei
Wu, Zongyi
Yao, Yongxiu
Qin, Aijian
Qian, Kun
author_sort Zhao, Wei
collection PubMed
description Chicken astrovirus (CAstV) infection is strongly associated with kidney disease, gout, “white chicks” hatchery disease, and runting and stunting syndrome (RSS). In the present study, 82.5% of 154 clinical samples from different provinces in China were positive for CAstV by RT-PCR. One CAstV isolate, designated as AAstV/Chicken/CHN/2017/NJ01, was successfully isolated from the small intestine of “Yellow” chickens using LMH cells. The genome sequence and structure analyses revealed that NJ1701 had the typical characteristics of avian astroviruses which was genetically distinct from other Avastrovirus. This isolate was classified as Group B subgroup i based on phylogenetic analysis of complete ORF2 (capsid) amino acid sequences. Meanwhile, growth depression and hatchability reduction were observed in the chicken embryo infection experiment. The results in the current study will contribute to our understanding of chicken astrovirus in China.
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spelling pubmed-76551282020-11-13 The Isolation and Molecular Characterization of an Astrovirus From “Yellow” Chickens, China Zhao, Wei Wu, Zongyi Yao, Yongxiu Qin, Aijian Qian, Kun Front Vet Sci Veterinary Science Chicken astrovirus (CAstV) infection is strongly associated with kidney disease, gout, “white chicks” hatchery disease, and runting and stunting syndrome (RSS). In the present study, 82.5% of 154 clinical samples from different provinces in China were positive for CAstV by RT-PCR. One CAstV isolate, designated as AAstV/Chicken/CHN/2017/NJ01, was successfully isolated from the small intestine of “Yellow” chickens using LMH cells. The genome sequence and structure analyses revealed that NJ1701 had the typical characteristics of avian astroviruses which was genetically distinct from other Avastrovirus. This isolate was classified as Group B subgroup i based on phylogenetic analysis of complete ORF2 (capsid) amino acid sequences. Meanwhile, growth depression and hatchability reduction were observed in the chicken embryo infection experiment. The results in the current study will contribute to our understanding of chicken astrovirus in China. Frontiers Media S.A. 2020-10-27 /pmc/articles/PMC7655128/ /pubmed/33195598 http://dx.doi.org/10.3389/fvets.2020.581862 Text en Copyright © 2020 Zhao, Wu, Yao, Qin and Qian. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Zhao, Wei
Wu, Zongyi
Yao, Yongxiu
Qin, Aijian
Qian, Kun
The Isolation and Molecular Characterization of an Astrovirus From “Yellow” Chickens, China
title The Isolation and Molecular Characterization of an Astrovirus From “Yellow” Chickens, China
title_full The Isolation and Molecular Characterization of an Astrovirus From “Yellow” Chickens, China
title_fullStr The Isolation and Molecular Characterization of an Astrovirus From “Yellow” Chickens, China
title_full_unstemmed The Isolation and Molecular Characterization of an Astrovirus From “Yellow” Chickens, China
title_short The Isolation and Molecular Characterization of an Astrovirus From “Yellow” Chickens, China
title_sort isolation and molecular characterization of an astrovirus from “yellow” chickens, china
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7655128/
https://www.ncbi.nlm.nih.gov/pubmed/33195598
http://dx.doi.org/10.3389/fvets.2020.581862
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