Cargando…
Identification of a competing endogenous RNA axis related to gastric cancer
Competing endogenous RNA (ceRNA) pathways play pivotal roles in the formation and progression of gastric cancer (GC). Employing multi-omics analysis, we sought to identify a ceRNA network associated with GC progression. We analyzed3Gene Expression Omnibus datasets as well as data from The Cancer Gen...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7655175/ https://www.ncbi.nlm.nih.gov/pubmed/33080572 http://dx.doi.org/10.18632/aging.103926 |
_version_ | 1783608186959298560 |
---|---|
author | Zu, Fuqiang Han, Hezhou Sheng, Weiwei Sun, Jian Zang, Hui Liang, Yu Liu, Qingfeng |
author_facet | Zu, Fuqiang Han, Hezhou Sheng, Weiwei Sun, Jian Zang, Hui Liang, Yu Liu, Qingfeng |
author_sort | Zu, Fuqiang |
collection | PubMed |
description | Competing endogenous RNA (ceRNA) pathways play pivotal roles in the formation and progression of gastric cancer (GC). Employing multi-omics analysis, we sought to identify a ceRNA network associated with GC progression. We analyzed3Gene Expression Omnibus datasets as well as data from The Cancer Genome Atlas to identify genes that were differentially expressed in GC tissues. A total of 84 upregulated genes and 106 downregulated genes were found. Enrichment analysis indicated that some pathways were strongly linked with tumor formation and progression. We also screened hub genes to establish a lncRNA-miRNA-mRNA network. We ultimately identified 8 hub genes, 6 key miRNAs and 4 key lncRNAs that interact within a common ceRNA network. Correlation analysis and in vitro experiments were conducted to verify the regulatory effect of the ceRNA network in GC. A knockdown assay confirmed that the DLGAP1-AS1/miR-203a-3p/THBS2 axis is a ceRNA network involved in GC progression. In this study, we elucidated the role of the DLGAP1-AS1/miR-203a-3p/THBS2 ceRNA network in the progression of GC. These molecules maybe evaluated as therapeutic targets and prognostic biomarkers for GC. |
format | Online Article Text |
id | pubmed-7655175 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Impact Journals |
record_format | MEDLINE/PubMed |
spelling | pubmed-76551752020-11-19 Identification of a competing endogenous RNA axis related to gastric cancer Zu, Fuqiang Han, Hezhou Sheng, Weiwei Sun, Jian Zang, Hui Liang, Yu Liu, Qingfeng Aging (Albany NY) Research Paper Competing endogenous RNA (ceRNA) pathways play pivotal roles in the formation and progression of gastric cancer (GC). Employing multi-omics analysis, we sought to identify a ceRNA network associated with GC progression. We analyzed3Gene Expression Omnibus datasets as well as data from The Cancer Genome Atlas to identify genes that were differentially expressed in GC tissues. A total of 84 upregulated genes and 106 downregulated genes were found. Enrichment analysis indicated that some pathways were strongly linked with tumor formation and progression. We also screened hub genes to establish a lncRNA-miRNA-mRNA network. We ultimately identified 8 hub genes, 6 key miRNAs and 4 key lncRNAs that interact within a common ceRNA network. Correlation analysis and in vitro experiments were conducted to verify the regulatory effect of the ceRNA network in GC. A knockdown assay confirmed that the DLGAP1-AS1/miR-203a-3p/THBS2 axis is a ceRNA network involved in GC progression. In this study, we elucidated the role of the DLGAP1-AS1/miR-203a-3p/THBS2 ceRNA network in the progression of GC. These molecules maybe evaluated as therapeutic targets and prognostic biomarkers for GC. Impact Journals 2020-10-20 /pmc/articles/PMC7655175/ /pubmed/33080572 http://dx.doi.org/10.18632/aging.103926 Text en Copyright: © 2020 Zu et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Zu, Fuqiang Han, Hezhou Sheng, Weiwei Sun, Jian Zang, Hui Liang, Yu Liu, Qingfeng Identification of a competing endogenous RNA axis related to gastric cancer |
title | Identification of a competing endogenous RNA axis related to gastric cancer |
title_full | Identification of a competing endogenous RNA axis related to gastric cancer |
title_fullStr | Identification of a competing endogenous RNA axis related to gastric cancer |
title_full_unstemmed | Identification of a competing endogenous RNA axis related to gastric cancer |
title_short | Identification of a competing endogenous RNA axis related to gastric cancer |
title_sort | identification of a competing endogenous rna axis related to gastric cancer |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7655175/ https://www.ncbi.nlm.nih.gov/pubmed/33080572 http://dx.doi.org/10.18632/aging.103926 |
work_keys_str_mv | AT zufuqiang identificationofacompetingendogenousrnaaxisrelatedtogastriccancer AT hanhezhou identificationofacompetingendogenousrnaaxisrelatedtogastriccancer AT shengweiwei identificationofacompetingendogenousrnaaxisrelatedtogastriccancer AT sunjian identificationofacompetingendogenousrnaaxisrelatedtogastriccancer AT zanghui identificationofacompetingendogenousrnaaxisrelatedtogastriccancer AT liangyu identificationofacompetingendogenousrnaaxisrelatedtogastriccancer AT liuqingfeng identificationofacompetingendogenousrnaaxisrelatedtogastriccancer |