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Dietary Fatty Acids in Postprandial Triglyceride-Rich Lipoproteins Modulate Human Monocyte-Derived Dendritic Cell Maturation and Activation

Dietary fatty acids have been demonstrated to modulate systemic inflammation and induce the postprandial inflammatory response of circulating immune cells. We hypothesized that postprandial triglyceride-rich lipoproteins (TRLs) may have acute effects on immunometabolic homeostasis by modulating dend...

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Autores principales: Vazquez-Madrigal, Carlos, Lopez, Soledad, Grao-Cruces, Elena, Millan-Linares, Maria C., Rodriguez-Martin, Noelia M., Martin, Maria E., Alba, Gonzalo, Santa-Maria, Consuelo, Bermudez, Beatriz, Montserrat-de la Paz, Sergio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7656296/
https://www.ncbi.nlm.nih.gov/pubmed/33066622
http://dx.doi.org/10.3390/nu12103139
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author Vazquez-Madrigal, Carlos
Lopez, Soledad
Grao-Cruces, Elena
Millan-Linares, Maria C.
Rodriguez-Martin, Noelia M.
Martin, Maria E.
Alba, Gonzalo
Santa-Maria, Consuelo
Bermudez, Beatriz
Montserrat-de la Paz, Sergio
author_facet Vazquez-Madrigal, Carlos
Lopez, Soledad
Grao-Cruces, Elena
Millan-Linares, Maria C.
Rodriguez-Martin, Noelia M.
Martin, Maria E.
Alba, Gonzalo
Santa-Maria, Consuelo
Bermudez, Beatriz
Montserrat-de la Paz, Sergio
author_sort Vazquez-Madrigal, Carlos
collection PubMed
description Dietary fatty acids have been demonstrated to modulate systemic inflammation and induce the postprandial inflammatory response of circulating immune cells. We hypothesized that postprandial triglyceride-rich lipoproteins (TRLs) may have acute effects on immunometabolic homeostasis by modulating dendritic cells (DCs), sentinels of the immunity that link innate and adaptive immune systems. In healthy volunteers, saturated fatty acid (SFA)-enriched meal raised serum levels of granulocyte/macrophage colony-stimulating factor GM-CSF (SFAs > monounsaturated fatty acids (MUFAs) = polyunsaturated fatty acids (PUFAs)) in the postprandial period. Autologous TRL-SFAs upregulated the gene expression of DC maturation (CD123 and CCR7) and DC pro-inflammatory activation (CD80 and CD86) genes while downregulating tolerogenic genes (PD-L1 and PD-L2) in human monocyte-derived DCs (moDCs). These effects were reversed with oleic acid-enriched TRLs. Moreover, postprandial SFAs raised IL-12p70 levels, while TRL-MUFAs and TRL-PUFAs increased IL-10 levels in serum of healthy volunteers and in the medium of TRL-treated moDCs. In conclusion, postprandial TRLs are metabolic entities with DC-related tolerogenic activity, and this function is linked to the type of dietary fat in the meal. This study shows that the intake of meals enriched in MUFAs from olive oil, when compared with meals enriched in SFAs, prevents the postprandial production and priming of circulating pro-inflammatory DCs, and promotes tolerogenic response in healthy subjects. However, functional assays with moDCs generated in the presence of different fatty acids and T cells could increase the knowledge of postprandial TRLs’ effects on DC differentiation and function.
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spelling pubmed-76562962020-11-12 Dietary Fatty Acids in Postprandial Triglyceride-Rich Lipoproteins Modulate Human Monocyte-Derived Dendritic Cell Maturation and Activation Vazquez-Madrigal, Carlos Lopez, Soledad Grao-Cruces, Elena Millan-Linares, Maria C. Rodriguez-Martin, Noelia M. Martin, Maria E. Alba, Gonzalo Santa-Maria, Consuelo Bermudez, Beatriz Montserrat-de la Paz, Sergio Nutrients Article Dietary fatty acids have been demonstrated to modulate systemic inflammation and induce the postprandial inflammatory response of circulating immune cells. We hypothesized that postprandial triglyceride-rich lipoproteins (TRLs) may have acute effects on immunometabolic homeostasis by modulating dendritic cells (DCs), sentinels of the immunity that link innate and adaptive immune systems. In healthy volunteers, saturated fatty acid (SFA)-enriched meal raised serum levels of granulocyte/macrophage colony-stimulating factor GM-CSF (SFAs > monounsaturated fatty acids (MUFAs) = polyunsaturated fatty acids (PUFAs)) in the postprandial period. Autologous TRL-SFAs upregulated the gene expression of DC maturation (CD123 and CCR7) and DC pro-inflammatory activation (CD80 and CD86) genes while downregulating tolerogenic genes (PD-L1 and PD-L2) in human monocyte-derived DCs (moDCs). These effects were reversed with oleic acid-enriched TRLs. Moreover, postprandial SFAs raised IL-12p70 levels, while TRL-MUFAs and TRL-PUFAs increased IL-10 levels in serum of healthy volunteers and in the medium of TRL-treated moDCs. In conclusion, postprandial TRLs are metabolic entities with DC-related tolerogenic activity, and this function is linked to the type of dietary fat in the meal. This study shows that the intake of meals enriched in MUFAs from olive oil, when compared with meals enriched in SFAs, prevents the postprandial production and priming of circulating pro-inflammatory DCs, and promotes tolerogenic response in healthy subjects. However, functional assays with moDCs generated in the presence of different fatty acids and T cells could increase the knowledge of postprandial TRLs’ effects on DC differentiation and function. MDPI 2020-10-14 /pmc/articles/PMC7656296/ /pubmed/33066622 http://dx.doi.org/10.3390/nu12103139 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vazquez-Madrigal, Carlos
Lopez, Soledad
Grao-Cruces, Elena
Millan-Linares, Maria C.
Rodriguez-Martin, Noelia M.
Martin, Maria E.
Alba, Gonzalo
Santa-Maria, Consuelo
Bermudez, Beatriz
Montserrat-de la Paz, Sergio
Dietary Fatty Acids in Postprandial Triglyceride-Rich Lipoproteins Modulate Human Monocyte-Derived Dendritic Cell Maturation and Activation
title Dietary Fatty Acids in Postprandial Triglyceride-Rich Lipoproteins Modulate Human Monocyte-Derived Dendritic Cell Maturation and Activation
title_full Dietary Fatty Acids in Postprandial Triglyceride-Rich Lipoproteins Modulate Human Monocyte-Derived Dendritic Cell Maturation and Activation
title_fullStr Dietary Fatty Acids in Postprandial Triglyceride-Rich Lipoproteins Modulate Human Monocyte-Derived Dendritic Cell Maturation and Activation
title_full_unstemmed Dietary Fatty Acids in Postprandial Triglyceride-Rich Lipoproteins Modulate Human Monocyte-Derived Dendritic Cell Maturation and Activation
title_short Dietary Fatty Acids in Postprandial Triglyceride-Rich Lipoproteins Modulate Human Monocyte-Derived Dendritic Cell Maturation and Activation
title_sort dietary fatty acids in postprandial triglyceride-rich lipoproteins modulate human monocyte-derived dendritic cell maturation and activation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7656296/
https://www.ncbi.nlm.nih.gov/pubmed/33066622
http://dx.doi.org/10.3390/nu12103139
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