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Duck enteritis virus pUL47, as a late structural protein localized in the nucleus, mainly depends on residues 40 to 50 and 768 to 777 and inhibits IFN-β signalling by interacting with STAT1
Duck enteritis virus (DEV) is a member of the Alphaherpesvirinae subfamily. The characteristics of some DEV genes have been reported. However, information regarding the DEV UL47 gene is limited. In this study, we identified the DEV UL47 gene encoding a late structural protein located in the nucleus...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2020
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7656727/ https://www.ncbi.nlm.nih.gov/pubmed/33176874 http://dx.doi.org/10.1186/s13567-020-00859-w |
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| author | He, Tianqiong Wang, Mingshu Cheng, Anchun Yang, Qiao Jia, Renyong Wu, Ying Huang, Juan Chen, Shun Zhao, Xin-Xin Liu, Mafeng Zhu, Dekang Zhang, Shaqiu Ou, Xuming Mao, Sai Gao, Qun Sun, Di Wen, XinJian Tian, Bin Liu, Yunya Yu, Yanling Zhang, Ling Pan, Leichang Chen, Xiaoyue |
| author_facet | He, Tianqiong Wang, Mingshu Cheng, Anchun Yang, Qiao Jia, Renyong Wu, Ying Huang, Juan Chen, Shun Zhao, Xin-Xin Liu, Mafeng Zhu, Dekang Zhang, Shaqiu Ou, Xuming Mao, Sai Gao, Qun Sun, Di Wen, XinJian Tian, Bin Liu, Yunya Yu, Yanling Zhang, Ling Pan, Leichang Chen, Xiaoyue |
| author_sort | He, Tianqiong |
| collection | PubMed |
| description | Duck enteritis virus (DEV) is a member of the Alphaherpesvirinae subfamily. The characteristics of some DEV genes have been reported. However, information regarding the DEV UL47 gene is limited. In this study, we identified the DEV UL47 gene encoding a late structural protein located in the nucleus of infected cells. We further found that two domains of DEV pUL47, amino acids (aa) 40 to 50 and 768 to 777, could function as nuclear localization sequence (NLS) to guide the nuclear localization of pUL47 and nuclear translocation of heterologous proteins, including enhanced green fluorescent protein (EGFP) and beta-galactosidase (β-Gal). Moreover, pUL47 significantly inhibited polyriboinosinic:polyribocytidylic acid [poly(I:C)]-induced interferon beta (IFN-β) production and downregulated interferon-stimulated gene (ISG) expression, such as Mx and oligoadenylate synthetase-like (OASL), by interacting with signal transducer and activator of transcription-1 (STAT1). |
| format | Online Article Text |
| id | pubmed-7656727 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2020 |
| publisher | BioMed Central |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-76567272020-11-13 Duck enteritis virus pUL47, as a late structural protein localized in the nucleus, mainly depends on residues 40 to 50 and 768 to 777 and inhibits IFN-β signalling by interacting with STAT1 He, Tianqiong Wang, Mingshu Cheng, Anchun Yang, Qiao Jia, Renyong Wu, Ying Huang, Juan Chen, Shun Zhao, Xin-Xin Liu, Mafeng Zhu, Dekang Zhang, Shaqiu Ou, Xuming Mao, Sai Gao, Qun Sun, Di Wen, XinJian Tian, Bin Liu, Yunya Yu, Yanling Zhang, Ling Pan, Leichang Chen, Xiaoyue Vet Res Research Article Duck enteritis virus (DEV) is a member of the Alphaherpesvirinae subfamily. The characteristics of some DEV genes have been reported. However, information regarding the DEV UL47 gene is limited. In this study, we identified the DEV UL47 gene encoding a late structural protein located in the nucleus of infected cells. We further found that two domains of DEV pUL47, amino acids (aa) 40 to 50 and 768 to 777, could function as nuclear localization sequence (NLS) to guide the nuclear localization of pUL47 and nuclear translocation of heterologous proteins, including enhanced green fluorescent protein (EGFP) and beta-galactosidase (β-Gal). Moreover, pUL47 significantly inhibited polyriboinosinic:polyribocytidylic acid [poly(I:C)]-induced interferon beta (IFN-β) production and downregulated interferon-stimulated gene (ISG) expression, such as Mx and oligoadenylate synthetase-like (OASL), by interacting with signal transducer and activator of transcription-1 (STAT1). BioMed Central 2020-11-11 2020 /pmc/articles/PMC7656727/ /pubmed/33176874 http://dx.doi.org/10.1186/s13567-020-00859-w Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
| spellingShingle | Research Article He, Tianqiong Wang, Mingshu Cheng, Anchun Yang, Qiao Jia, Renyong Wu, Ying Huang, Juan Chen, Shun Zhao, Xin-Xin Liu, Mafeng Zhu, Dekang Zhang, Shaqiu Ou, Xuming Mao, Sai Gao, Qun Sun, Di Wen, XinJian Tian, Bin Liu, Yunya Yu, Yanling Zhang, Ling Pan, Leichang Chen, Xiaoyue Duck enteritis virus pUL47, as a late structural protein localized in the nucleus, mainly depends on residues 40 to 50 and 768 to 777 and inhibits IFN-β signalling by interacting with STAT1 |
| title | Duck enteritis virus pUL47, as a late structural protein localized in the nucleus, mainly depends on residues 40 to 50 and 768 to 777 and inhibits IFN-β signalling by interacting with STAT1 |
| title_full | Duck enteritis virus pUL47, as a late structural protein localized in the nucleus, mainly depends on residues 40 to 50 and 768 to 777 and inhibits IFN-β signalling by interacting with STAT1 |
| title_fullStr | Duck enteritis virus pUL47, as a late structural protein localized in the nucleus, mainly depends on residues 40 to 50 and 768 to 777 and inhibits IFN-β signalling by interacting with STAT1 |
| title_full_unstemmed | Duck enteritis virus pUL47, as a late structural protein localized in the nucleus, mainly depends on residues 40 to 50 and 768 to 777 and inhibits IFN-β signalling by interacting with STAT1 |
| title_short | Duck enteritis virus pUL47, as a late structural protein localized in the nucleus, mainly depends on residues 40 to 50 and 768 to 777 and inhibits IFN-β signalling by interacting with STAT1 |
| title_sort | duck enteritis virus pul47, as a late structural protein localized in the nucleus, mainly depends on residues 40 to 50 and 768 to 777 and inhibits ifn-β signalling by interacting with stat1 |
| topic | Research Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7656727/ https://www.ncbi.nlm.nih.gov/pubmed/33176874 http://dx.doi.org/10.1186/s13567-020-00859-w |
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