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Porous polylactic acid scaffolds for bone regeneration: A study of additively manufactured triply periodic minimal surfaces and their osteogenic potential
Three different triply periodic minimal surfaces (TPMS) with three levels of porosity within those of cancellous bone were investigated as potential bone scaffolds. TPMS have emerged as potential designs to resemble the complex mechanical and mass transport properties of bone. Diamond, Schwarz, and...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7656876/ https://www.ncbi.nlm.nih.gov/pubmed/33224463 http://dx.doi.org/10.1177/2041731420956541 |
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author | Diez-Escudero, Anna Harlin, Hugo Isaksson, Per Persson, Cecilia |
author_facet | Diez-Escudero, Anna Harlin, Hugo Isaksson, Per Persson, Cecilia |
author_sort | Diez-Escudero, Anna |
collection | PubMed |
description | Three different triply periodic minimal surfaces (TPMS) with three levels of porosity within those of cancellous bone were investigated as potential bone scaffolds. TPMS have emerged as potential designs to resemble the complex mechanical and mass transport properties of bone. Diamond, Schwarz, and Gyroid structures were 3D printed in polylactic acid, a resorbable medical grade material. The 3D printed structures were investigated for printing feasibility, and assessed by morphometric studies. Mechanical properties and permeability investigations resulted in similar values to cancellous bone. The morphometric analyses showed three different patterns of pore distribution: mono-, bi-, and multimodal pores. Subsequently, biological activity investigated with pre-osteoblastic cell lines showed no signs of cytotoxicity, and the scaffolds supported cell proliferation up to 3 weeks. Cell differentiation investigated by alkaline phosphatase showed an improvement for higher porosities and multimodal pore distributions, suggesting a higher dependency on pore distribution and size than the level of interconnectivity. |
format | Online Article Text |
id | pubmed-7656876 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-76568762020-11-20 Porous polylactic acid scaffolds for bone regeneration: A study of additively manufactured triply periodic minimal surfaces and their osteogenic potential Diez-Escudero, Anna Harlin, Hugo Isaksson, Per Persson, Cecilia J Tissue Eng Design and Manufacture of Tissue Engineered Products using Additive Manufacturing Techniques Three different triply periodic minimal surfaces (TPMS) with three levels of porosity within those of cancellous bone were investigated as potential bone scaffolds. TPMS have emerged as potential designs to resemble the complex mechanical and mass transport properties of bone. Diamond, Schwarz, and Gyroid structures were 3D printed in polylactic acid, a resorbable medical grade material. The 3D printed structures were investigated for printing feasibility, and assessed by morphometric studies. Mechanical properties and permeability investigations resulted in similar values to cancellous bone. The morphometric analyses showed three different patterns of pore distribution: mono-, bi-, and multimodal pores. Subsequently, biological activity investigated with pre-osteoblastic cell lines showed no signs of cytotoxicity, and the scaffolds supported cell proliferation up to 3 weeks. Cell differentiation investigated by alkaline phosphatase showed an improvement for higher porosities and multimodal pore distributions, suggesting a higher dependency on pore distribution and size than the level of interconnectivity. SAGE Publications 2020-11-06 /pmc/articles/PMC7656876/ /pubmed/33224463 http://dx.doi.org/10.1177/2041731420956541 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution 4.0 License (https://creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Design and Manufacture of Tissue Engineered Products using Additive Manufacturing Techniques Diez-Escudero, Anna Harlin, Hugo Isaksson, Per Persson, Cecilia Porous polylactic acid scaffolds for bone regeneration: A study of additively manufactured triply periodic minimal surfaces and their osteogenic potential |
title | Porous polylactic acid scaffolds for bone regeneration: A study of additively manufactured triply periodic minimal surfaces and their osteogenic potential |
title_full | Porous polylactic acid scaffolds for bone regeneration: A study of additively manufactured triply periodic minimal surfaces and their osteogenic potential |
title_fullStr | Porous polylactic acid scaffolds for bone regeneration: A study of additively manufactured triply periodic minimal surfaces and their osteogenic potential |
title_full_unstemmed | Porous polylactic acid scaffolds for bone regeneration: A study of additively manufactured triply periodic minimal surfaces and their osteogenic potential |
title_short | Porous polylactic acid scaffolds for bone regeneration: A study of additively manufactured triply periodic minimal surfaces and their osteogenic potential |
title_sort | porous polylactic acid scaffolds for bone regeneration: a study of additively manufactured triply periodic minimal surfaces and their osteogenic potential |
topic | Design and Manufacture of Tissue Engineered Products using Additive Manufacturing Techniques |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7656876/ https://www.ncbi.nlm.nih.gov/pubmed/33224463 http://dx.doi.org/10.1177/2041731420956541 |
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