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The Ankyrin-Repeat Gene GmANK114 Confers Drought and Salt Tolerance in Arabidopsis and Soybean

Ankyrin repeat (ANK) proteins are essential in cell growth, development, and response to hormones and environmental stresses. In the present study, 226 ANK genes were identified and classified into nine subfamilies according to conserved domains in the soybean genome (Glycine max L.). Among them, th...

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Autores principales: Zhao, Juan-Ying, Lu, Zhi-Wei, Sun, Yue, Fang, Zheng-Wu, Chen, Jun, Zhou, Yong-Bin, Chen, Ming, Ma, You-Zhi, Xu, Zhao-Shi, Min, Dong-Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7658197/
https://www.ncbi.nlm.nih.gov/pubmed/33193533
http://dx.doi.org/10.3389/fpls.2020.584167
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author Zhao, Juan-Ying
Lu, Zhi-Wei
Sun, Yue
Fang, Zheng-Wu
Chen, Jun
Zhou, Yong-Bin
Chen, Ming
Ma, You-Zhi
Xu, Zhao-Shi
Min, Dong-Hong
author_facet Zhao, Juan-Ying
Lu, Zhi-Wei
Sun, Yue
Fang, Zheng-Wu
Chen, Jun
Zhou, Yong-Bin
Chen, Ming
Ma, You-Zhi
Xu, Zhao-Shi
Min, Dong-Hong
author_sort Zhao, Juan-Ying
collection PubMed
description Ankyrin repeat (ANK) proteins are essential in cell growth, development, and response to hormones and environmental stresses. In the present study, 226 ANK genes were identified and classified into nine subfamilies according to conserved domains in the soybean genome (Glycine max L.). Among them, the GmANK114 was highly induced by drought, salt, and abscisic acid. The GmANK114 encodes a protein that belongs to the ANK-RF subfamily containing a RING finger (RF) domain in addition to the ankyrin repeats. Heterologous overexpression of GmANK114 in transgenic Arabidopsis improved the germination rate under drought and salt treatments compared to wild-type. Homologous overexpression of GmANK114 improved the survival rate under drought and salt stresses in transgenic soybean hairy roots. In response to drought or salt stress, GmANK114 overexpression in soybean hairy root showed higher proline and lower malondialdehyde contents, and lower H(2)O(2) and O(2–) contents compared control plants. Besides, GmANK114 activated transcription of several abiotic stress-related genes, including WRKY13, NAC11, DREB2, MYB84, and bZIP44 under drought and salt stresses in soybean. These results provide new insights for functional analysis of soybean ANK proteins and will be helpful for further understanding how ANK proteins in plants adapt to abiotic stress.
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spelling pubmed-76581972020-11-13 The Ankyrin-Repeat Gene GmANK114 Confers Drought and Salt Tolerance in Arabidopsis and Soybean Zhao, Juan-Ying Lu, Zhi-Wei Sun, Yue Fang, Zheng-Wu Chen, Jun Zhou, Yong-Bin Chen, Ming Ma, You-Zhi Xu, Zhao-Shi Min, Dong-Hong Front Plant Sci Plant Science Ankyrin repeat (ANK) proteins are essential in cell growth, development, and response to hormones and environmental stresses. In the present study, 226 ANK genes were identified and classified into nine subfamilies according to conserved domains in the soybean genome (Glycine max L.). Among them, the GmANK114 was highly induced by drought, salt, and abscisic acid. The GmANK114 encodes a protein that belongs to the ANK-RF subfamily containing a RING finger (RF) domain in addition to the ankyrin repeats. Heterologous overexpression of GmANK114 in transgenic Arabidopsis improved the germination rate under drought and salt treatments compared to wild-type. Homologous overexpression of GmANK114 improved the survival rate under drought and salt stresses in transgenic soybean hairy roots. In response to drought or salt stress, GmANK114 overexpression in soybean hairy root showed higher proline and lower malondialdehyde contents, and lower H(2)O(2) and O(2–) contents compared control plants. Besides, GmANK114 activated transcription of several abiotic stress-related genes, including WRKY13, NAC11, DREB2, MYB84, and bZIP44 under drought and salt stresses in soybean. These results provide new insights for functional analysis of soybean ANK proteins and will be helpful for further understanding how ANK proteins in plants adapt to abiotic stress. Frontiers Media S.A. 2020-10-29 /pmc/articles/PMC7658197/ /pubmed/33193533 http://dx.doi.org/10.3389/fpls.2020.584167 Text en Copyright © 2020 Zhao, Lu, Sun, Fang, Chen, Zhou, Chen, Ma, Xu and Min. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Zhao, Juan-Ying
Lu, Zhi-Wei
Sun, Yue
Fang, Zheng-Wu
Chen, Jun
Zhou, Yong-Bin
Chen, Ming
Ma, You-Zhi
Xu, Zhao-Shi
Min, Dong-Hong
The Ankyrin-Repeat Gene GmANK114 Confers Drought and Salt Tolerance in Arabidopsis and Soybean
title The Ankyrin-Repeat Gene GmANK114 Confers Drought and Salt Tolerance in Arabidopsis and Soybean
title_full The Ankyrin-Repeat Gene GmANK114 Confers Drought and Salt Tolerance in Arabidopsis and Soybean
title_fullStr The Ankyrin-Repeat Gene GmANK114 Confers Drought and Salt Tolerance in Arabidopsis and Soybean
title_full_unstemmed The Ankyrin-Repeat Gene GmANK114 Confers Drought and Salt Tolerance in Arabidopsis and Soybean
title_short The Ankyrin-Repeat Gene GmANK114 Confers Drought and Salt Tolerance in Arabidopsis and Soybean
title_sort ankyrin-repeat gene gmank114 confers drought and salt tolerance in arabidopsis and soybean
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7658197/
https://www.ncbi.nlm.nih.gov/pubmed/33193533
http://dx.doi.org/10.3389/fpls.2020.584167
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