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Molecular insights into the mechanisms of susceptibility of Labeo rohita against oomycete Aphanomyces invadans

Aphanomyces invadans, the causative agent of epizootic ulcerative syndrome, is one of the most destructive pathogens of freshwater fishes. To date, the disease has been reported from over 160 fish species in 20 countries and notably, this is the first non-salmonid disease that has resulted in major...

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Autores principales: Pradhan, P. K., Verma, Dev Kumar, Peruzza, Luca, Gupta, Shubham, Haq, Syed Assim, Shubin, Sergei V., Morgan, Kenton L., Trusch, Franziska, Mohindra, Vindhya, Hauton, Chris, van West, Pieter, Sood, Neeraj
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7658212/
https://www.ncbi.nlm.nih.gov/pubmed/33177569
http://dx.doi.org/10.1038/s41598-020-76278-w
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author Pradhan, P. K.
Verma, Dev Kumar
Peruzza, Luca
Gupta, Shubham
Haq, Syed Assim
Shubin, Sergei V.
Morgan, Kenton L.
Trusch, Franziska
Mohindra, Vindhya
Hauton, Chris
van West, Pieter
Sood, Neeraj
author_facet Pradhan, P. K.
Verma, Dev Kumar
Peruzza, Luca
Gupta, Shubham
Haq, Syed Assim
Shubin, Sergei V.
Morgan, Kenton L.
Trusch, Franziska
Mohindra, Vindhya
Hauton, Chris
van West, Pieter
Sood, Neeraj
author_sort Pradhan, P. K.
collection PubMed
description Aphanomyces invadans, the causative agent of epizootic ulcerative syndrome, is one of the most destructive pathogens of freshwater fishes. To date, the disease has been reported from over 160 fish species in 20 countries and notably, this is the first non-salmonid disease that has resulted in major impacts globally. In particular, Indian major carps (IMCs) are highly susceptible to this disease. To increase our knowledge particularly with regards to host immune response against A. invadans infection in a susceptible host, the gene expression profile in head kidney of A. invadans-infected and control rohu, Labeo rohita was investigated using RNA sequencing. Time course analysis of RNA-Seq data revealed 5608 differentially expressed genes, involved among others in Antigen processing and presentation, Leukocyte transendothelial migration, IL-17 signaling, Chemokine signaling, C-type lectin receptor signaling and Toll-like receptor signaling pathways. In the affected pathways, a number of immune genes were found to be downregulated, suggesting an immune evasion strategy of A. invadans in establishing the infection. The information generated in this study offers first systematic mechanistic understanding of the host–pathogen interaction that might underpin the development of new management strategies for this economically devastating fish-pathogenic oomycete A. invadans.
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spelling pubmed-76582122020-11-12 Molecular insights into the mechanisms of susceptibility of Labeo rohita against oomycete Aphanomyces invadans Pradhan, P. K. Verma, Dev Kumar Peruzza, Luca Gupta, Shubham Haq, Syed Assim Shubin, Sergei V. Morgan, Kenton L. Trusch, Franziska Mohindra, Vindhya Hauton, Chris van West, Pieter Sood, Neeraj Sci Rep Article Aphanomyces invadans, the causative agent of epizootic ulcerative syndrome, is one of the most destructive pathogens of freshwater fishes. To date, the disease has been reported from over 160 fish species in 20 countries and notably, this is the first non-salmonid disease that has resulted in major impacts globally. In particular, Indian major carps (IMCs) are highly susceptible to this disease. To increase our knowledge particularly with regards to host immune response against A. invadans infection in a susceptible host, the gene expression profile in head kidney of A. invadans-infected and control rohu, Labeo rohita was investigated using RNA sequencing. Time course analysis of RNA-Seq data revealed 5608 differentially expressed genes, involved among others in Antigen processing and presentation, Leukocyte transendothelial migration, IL-17 signaling, Chemokine signaling, C-type lectin receptor signaling and Toll-like receptor signaling pathways. In the affected pathways, a number of immune genes were found to be downregulated, suggesting an immune evasion strategy of A. invadans in establishing the infection. The information generated in this study offers first systematic mechanistic understanding of the host–pathogen interaction that might underpin the development of new management strategies for this economically devastating fish-pathogenic oomycete A. invadans. Nature Publishing Group UK 2020-11-11 /pmc/articles/PMC7658212/ /pubmed/33177569 http://dx.doi.org/10.1038/s41598-020-76278-w Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Pradhan, P. K.
Verma, Dev Kumar
Peruzza, Luca
Gupta, Shubham
Haq, Syed Assim
Shubin, Sergei V.
Morgan, Kenton L.
Trusch, Franziska
Mohindra, Vindhya
Hauton, Chris
van West, Pieter
Sood, Neeraj
Molecular insights into the mechanisms of susceptibility of Labeo rohita against oomycete Aphanomyces invadans
title Molecular insights into the mechanisms of susceptibility of Labeo rohita against oomycete Aphanomyces invadans
title_full Molecular insights into the mechanisms of susceptibility of Labeo rohita against oomycete Aphanomyces invadans
title_fullStr Molecular insights into the mechanisms of susceptibility of Labeo rohita against oomycete Aphanomyces invadans
title_full_unstemmed Molecular insights into the mechanisms of susceptibility of Labeo rohita against oomycete Aphanomyces invadans
title_short Molecular insights into the mechanisms of susceptibility of Labeo rohita against oomycete Aphanomyces invadans
title_sort molecular insights into the mechanisms of susceptibility of labeo rohita against oomycete aphanomyces invadans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7658212/
https://www.ncbi.nlm.nih.gov/pubmed/33177569
http://dx.doi.org/10.1038/s41598-020-76278-w
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