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The role of soluble mediators in the clinical course of EBV infection and B cell homeostasis after kidney transplantation
Epstein-Barr virus (EBV) reactivation can lead to serious complications in kidney transplant patients, including post-transplant lymphoproliferative disorder (PTLD). Here, we have assessed the impact of EBV on B cell homeostasis at cellular and humoral level. In a multicenter study monitoring 540 ki...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7658229/ https://www.ncbi.nlm.nih.gov/pubmed/33177622 http://dx.doi.org/10.1038/s41598-020-76607-z |
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author | Bajda, Sharon Blazquez-Navarro, Arturo Samans, Björn Wehler, Patrizia Kaliszczyk, Sviatlana Amini, Leila Schmueck-Henneresse, Michael Witzke, Oliver Dittmer, Ulf Westhoff, Timm H. Viebahn, Richard Reinke, Petra Thomusch, Oliver Hugo, Christian Olek, Sven Roch, Toralf Babel, Nina |
author_facet | Bajda, Sharon Blazquez-Navarro, Arturo Samans, Björn Wehler, Patrizia Kaliszczyk, Sviatlana Amini, Leila Schmueck-Henneresse, Michael Witzke, Oliver Dittmer, Ulf Westhoff, Timm H. Viebahn, Richard Reinke, Petra Thomusch, Oliver Hugo, Christian Olek, Sven Roch, Toralf Babel, Nina |
author_sort | Bajda, Sharon |
collection | PubMed |
description | Epstein-Barr virus (EBV) reactivation can lead to serious complications in kidney transplant patients, including post-transplant lymphoproliferative disorder (PTLD). Here, we have assessed the impact of EBV on B cell homeostasis at cellular and humoral level. In a multicenter study monitoring 540 kidney transplant patients during the first post-transplant year, EBV reactivation was detected in 109 patients. Thirteen soluble factors and B cell counts were analyzed in an EBV(+) sub-cohort (N = 54) before, at peak and after EBV clearance and compared to a control group (N = 50). The B cell activating factor (BAFF) was significantly elevated among EBV(+) patients. No additional soluble factors were associated with EBV. Importantly, in vitro experiments confirmed the proliferative effect of BAFF on EBV-infected B cells, simultaneously promoting EBV production. In contrast, elevated levels of BAFF in EBV(+) patients did not lead to B cell expansion in vivo. Moreover, diminished positive inter-correlations of soluble factors and alterations of the bi-directional interplay between B cell and soluble factors were observed in EBV(+) patients at peak and after clearance. Our data suggest that such alterations may counteract the proliferative effect of BAFF, preventing B cell expansion. The role of these alterations in lymphoma development should be analyzed in future studies. |
format | Online Article Text |
id | pubmed-7658229 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-76582292020-11-12 The role of soluble mediators in the clinical course of EBV infection and B cell homeostasis after kidney transplantation Bajda, Sharon Blazquez-Navarro, Arturo Samans, Björn Wehler, Patrizia Kaliszczyk, Sviatlana Amini, Leila Schmueck-Henneresse, Michael Witzke, Oliver Dittmer, Ulf Westhoff, Timm H. Viebahn, Richard Reinke, Petra Thomusch, Oliver Hugo, Christian Olek, Sven Roch, Toralf Babel, Nina Sci Rep Article Epstein-Barr virus (EBV) reactivation can lead to serious complications in kidney transplant patients, including post-transplant lymphoproliferative disorder (PTLD). Here, we have assessed the impact of EBV on B cell homeostasis at cellular and humoral level. In a multicenter study monitoring 540 kidney transplant patients during the first post-transplant year, EBV reactivation was detected in 109 patients. Thirteen soluble factors and B cell counts were analyzed in an EBV(+) sub-cohort (N = 54) before, at peak and after EBV clearance and compared to a control group (N = 50). The B cell activating factor (BAFF) was significantly elevated among EBV(+) patients. No additional soluble factors were associated with EBV. Importantly, in vitro experiments confirmed the proliferative effect of BAFF on EBV-infected B cells, simultaneously promoting EBV production. In contrast, elevated levels of BAFF in EBV(+) patients did not lead to B cell expansion in vivo. Moreover, diminished positive inter-correlations of soluble factors and alterations of the bi-directional interplay between B cell and soluble factors were observed in EBV(+) patients at peak and after clearance. Our data suggest that such alterations may counteract the proliferative effect of BAFF, preventing B cell expansion. The role of these alterations in lymphoma development should be analyzed in future studies. Nature Publishing Group UK 2020-11-11 /pmc/articles/PMC7658229/ /pubmed/33177622 http://dx.doi.org/10.1038/s41598-020-76607-z Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Bajda, Sharon Blazquez-Navarro, Arturo Samans, Björn Wehler, Patrizia Kaliszczyk, Sviatlana Amini, Leila Schmueck-Henneresse, Michael Witzke, Oliver Dittmer, Ulf Westhoff, Timm H. Viebahn, Richard Reinke, Petra Thomusch, Oliver Hugo, Christian Olek, Sven Roch, Toralf Babel, Nina The role of soluble mediators in the clinical course of EBV infection and B cell homeostasis after kidney transplantation |
title | The role of soluble mediators in the clinical course of EBV infection and B cell homeostasis after kidney transplantation |
title_full | The role of soluble mediators in the clinical course of EBV infection and B cell homeostasis after kidney transplantation |
title_fullStr | The role of soluble mediators in the clinical course of EBV infection and B cell homeostasis after kidney transplantation |
title_full_unstemmed | The role of soluble mediators in the clinical course of EBV infection and B cell homeostasis after kidney transplantation |
title_short | The role of soluble mediators in the clinical course of EBV infection and B cell homeostasis after kidney transplantation |
title_sort | role of soluble mediators in the clinical course of ebv infection and b cell homeostasis after kidney transplantation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7658229/ https://www.ncbi.nlm.nih.gov/pubmed/33177622 http://dx.doi.org/10.1038/s41598-020-76607-z |
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