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LHH1, a novel antimicrobial peptide with anti-cancer cell activity identified from Lactobacillus casei HZ1

Antimicrobial peptides have been attracting increasing attention for their multiple beneficial effects. In present study, a novel AMP with a molecular weight of 1875.5 Da, was identified from the genome of Lactobacillus casei HZ1. The peptide, which was named as LHH1 was comprised of 16 amino acid r...

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Autores principales: He, Jun-Fang, Jin, Du-Xin, Luo, Xue-Gang, Zhang, Tong-Cun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7658291/
https://www.ncbi.nlm.nih.gov/pubmed/33175275
http://dx.doi.org/10.1186/s13568-020-01139-8
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author He, Jun-Fang
Jin, Du-Xin
Luo, Xue-Gang
Zhang, Tong-Cun
author_facet He, Jun-Fang
Jin, Du-Xin
Luo, Xue-Gang
Zhang, Tong-Cun
author_sort He, Jun-Fang
collection PubMed
description Antimicrobial peptides have been attracting increasing attention for their multiple beneficial effects. In present study, a novel AMP with a molecular weight of 1875.5 Da, was identified from the genome of Lactobacillus casei HZ1. The peptide, which was named as LHH1 was comprised of 16 amino acid residues, and its α-helix content was 95.34% when dissolved in 30 mM SDS. LHH1 exhibited a broad range of antimicrobial activities against Gram-positive bacteria and fungus. It could effectively inhibit Staphylococcus aureus with a minimum inhibitory concentration of 3.5 μM and showed a low hemolytic activity. The scanning electron microscope, confocal laser scanning microscope and flow cytometry results showed that LHH1 exerted its antibacterial activity by damaging the cell membrane of Staphylococcus aureus. Meanwhile, LHH1 also exhibited anti-cancer cell activities against several cancer cells via breaking the cell membrane of MGC803, HCT116 and C666-1 cancer cells.
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spelling pubmed-76582912020-11-16 LHH1, a novel antimicrobial peptide with anti-cancer cell activity identified from Lactobacillus casei HZ1 He, Jun-Fang Jin, Du-Xin Luo, Xue-Gang Zhang, Tong-Cun AMB Express Original Article Antimicrobial peptides have been attracting increasing attention for their multiple beneficial effects. In present study, a novel AMP with a molecular weight of 1875.5 Da, was identified from the genome of Lactobacillus casei HZ1. The peptide, which was named as LHH1 was comprised of 16 amino acid residues, and its α-helix content was 95.34% when dissolved in 30 mM SDS. LHH1 exhibited a broad range of antimicrobial activities against Gram-positive bacteria and fungus. It could effectively inhibit Staphylococcus aureus with a minimum inhibitory concentration of 3.5 μM and showed a low hemolytic activity. The scanning electron microscope, confocal laser scanning microscope and flow cytometry results showed that LHH1 exerted its antibacterial activity by damaging the cell membrane of Staphylococcus aureus. Meanwhile, LHH1 also exhibited anti-cancer cell activities against several cancer cells via breaking the cell membrane of MGC803, HCT116 and C666-1 cancer cells. Springer Berlin Heidelberg 2020-11-11 /pmc/articles/PMC7658291/ /pubmed/33175275 http://dx.doi.org/10.1186/s13568-020-01139-8 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Original Article
He, Jun-Fang
Jin, Du-Xin
Luo, Xue-Gang
Zhang, Tong-Cun
LHH1, a novel antimicrobial peptide with anti-cancer cell activity identified from Lactobacillus casei HZ1
title LHH1, a novel antimicrobial peptide with anti-cancer cell activity identified from Lactobacillus casei HZ1
title_full LHH1, a novel antimicrobial peptide with anti-cancer cell activity identified from Lactobacillus casei HZ1
title_fullStr LHH1, a novel antimicrobial peptide with anti-cancer cell activity identified from Lactobacillus casei HZ1
title_full_unstemmed LHH1, a novel antimicrobial peptide with anti-cancer cell activity identified from Lactobacillus casei HZ1
title_short LHH1, a novel antimicrobial peptide with anti-cancer cell activity identified from Lactobacillus casei HZ1
title_sort lhh1, a novel antimicrobial peptide with anti-cancer cell activity identified from lactobacillus casei hz1
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7658291/
https://www.ncbi.nlm.nih.gov/pubmed/33175275
http://dx.doi.org/10.1186/s13568-020-01139-8
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