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A 3D-bioprinted scaffold with doxycycline-controlled BMP2-expressing cells for inducing bone regeneration and inhibiting bacterial infection
Large bone defects face a high risk of pathogen exposure due to open wounds, which leads to high infection rates and delayed bone union. To promote successful repair of infectious bone defects, fabrication of a scaffold with dual functions of osteo-induction and bacterial inhibition is required. Thi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
KeAi Publishing
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7658329/ https://www.ncbi.nlm.nih.gov/pubmed/33210025 http://dx.doi.org/10.1016/j.bioactmat.2020.10.022 |
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author | Wang, Minqi Li, Hanjun Yang, Yiqi Yuan, Kai Zhou, Feng Liu, Haibei Zhou, Qinghui Yang, Shengbing Tang, Tingting |
author_facet | Wang, Minqi Li, Hanjun Yang, Yiqi Yuan, Kai Zhou, Feng Liu, Haibei Zhou, Qinghui Yang, Shengbing Tang, Tingting |
author_sort | Wang, Minqi |
collection | PubMed |
description | Large bone defects face a high risk of pathogen exposure due to open wounds, which leads to high infection rates and delayed bone union. To promote successful repair of infectious bone defects, fabrication of a scaffold with dual functions of osteo-induction and bacterial inhibition is required. This study describes creation of an engineered progenitor cell line (C3H10T1/2) capable of doxycycline (DOX)-mediated release of bone morphogenetic protein-2 (BMP2). Three-dimensional bioprinting technology enabled creation of scaffolds, comprising polycaprolactone/mesoporous bioactive glass/DOX and bioink, containing these engineered cells. In vivo and in vitro experiments confirmed that the scaffold could actively secrete BMP2 to significantly promote osteoblast differentiation and induce ectopic bone formation. Additionally, the scaffold exhibited broad-spectrum antibacterial capacity, thereby ensuring the survival of embedded engineered cells when facing high risk of infection. These findings demonstrated the efficacy of this bioprinted scaffold to release BMP2 in a controlled manner and prevent the occurrence of infection; thus, showing its potential for repairing infectious bone defects. |
format | Online Article Text |
id | pubmed-7658329 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | KeAi Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-76583292020-11-17 A 3D-bioprinted scaffold with doxycycline-controlled BMP2-expressing cells for inducing bone regeneration and inhibiting bacterial infection Wang, Minqi Li, Hanjun Yang, Yiqi Yuan, Kai Zhou, Feng Liu, Haibei Zhou, Qinghui Yang, Shengbing Tang, Tingting Bioact Mater Article Large bone defects face a high risk of pathogen exposure due to open wounds, which leads to high infection rates and delayed bone union. To promote successful repair of infectious bone defects, fabrication of a scaffold with dual functions of osteo-induction and bacterial inhibition is required. This study describes creation of an engineered progenitor cell line (C3H10T1/2) capable of doxycycline (DOX)-mediated release of bone morphogenetic protein-2 (BMP2). Three-dimensional bioprinting technology enabled creation of scaffolds, comprising polycaprolactone/mesoporous bioactive glass/DOX and bioink, containing these engineered cells. In vivo and in vitro experiments confirmed that the scaffold could actively secrete BMP2 to significantly promote osteoblast differentiation and induce ectopic bone formation. Additionally, the scaffold exhibited broad-spectrum antibacterial capacity, thereby ensuring the survival of embedded engineered cells when facing high risk of infection. These findings demonstrated the efficacy of this bioprinted scaffold to release BMP2 in a controlled manner and prevent the occurrence of infection; thus, showing its potential for repairing infectious bone defects. KeAi Publishing 2020-11-10 /pmc/articles/PMC7658329/ /pubmed/33210025 http://dx.doi.org/10.1016/j.bioactmat.2020.10.022 Text en © 2020 [The Author/The Authors] http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Wang, Minqi Li, Hanjun Yang, Yiqi Yuan, Kai Zhou, Feng Liu, Haibei Zhou, Qinghui Yang, Shengbing Tang, Tingting A 3D-bioprinted scaffold with doxycycline-controlled BMP2-expressing cells for inducing bone regeneration and inhibiting bacterial infection |
title | A 3D-bioprinted scaffold with doxycycline-controlled BMP2-expressing cells for inducing bone regeneration and inhibiting bacterial infection |
title_full | A 3D-bioprinted scaffold with doxycycline-controlled BMP2-expressing cells for inducing bone regeneration and inhibiting bacterial infection |
title_fullStr | A 3D-bioprinted scaffold with doxycycline-controlled BMP2-expressing cells for inducing bone regeneration and inhibiting bacterial infection |
title_full_unstemmed | A 3D-bioprinted scaffold with doxycycline-controlled BMP2-expressing cells for inducing bone regeneration and inhibiting bacterial infection |
title_short | A 3D-bioprinted scaffold with doxycycline-controlled BMP2-expressing cells for inducing bone regeneration and inhibiting bacterial infection |
title_sort | 3d-bioprinted scaffold with doxycycline-controlled bmp2-expressing cells for inducing bone regeneration and inhibiting bacterial infection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7658329/ https://www.ncbi.nlm.nih.gov/pubmed/33210025 http://dx.doi.org/10.1016/j.bioactmat.2020.10.022 |
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