Cargando…

A 3D-bioprinted scaffold with doxycycline-controlled BMP2-expressing cells for inducing bone regeneration and inhibiting bacterial infection

Large bone defects face a high risk of pathogen exposure due to open wounds, which leads to high infection rates and delayed bone union. To promote successful repair of infectious bone defects, fabrication of a scaffold with dual functions of osteo-induction and bacterial inhibition is required. Thi...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Minqi, Li, Hanjun, Yang, Yiqi, Yuan, Kai, Zhou, Feng, Liu, Haibei, Zhou, Qinghui, Yang, Shengbing, Tang, Tingting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7658329/
https://www.ncbi.nlm.nih.gov/pubmed/33210025
http://dx.doi.org/10.1016/j.bioactmat.2020.10.022
_version_ 1783608645976588288
author Wang, Minqi
Li, Hanjun
Yang, Yiqi
Yuan, Kai
Zhou, Feng
Liu, Haibei
Zhou, Qinghui
Yang, Shengbing
Tang, Tingting
author_facet Wang, Minqi
Li, Hanjun
Yang, Yiqi
Yuan, Kai
Zhou, Feng
Liu, Haibei
Zhou, Qinghui
Yang, Shengbing
Tang, Tingting
author_sort Wang, Minqi
collection PubMed
description Large bone defects face a high risk of pathogen exposure due to open wounds, which leads to high infection rates and delayed bone union. To promote successful repair of infectious bone defects, fabrication of a scaffold with dual functions of osteo-induction and bacterial inhibition is required. This study describes creation of an engineered progenitor cell line (C3H10T1/2) capable of doxycycline (DOX)-mediated release of bone morphogenetic protein-2 (BMP2). Three-dimensional bioprinting technology enabled creation of scaffolds, comprising polycaprolactone/mesoporous bioactive glass/DOX and bioink, containing these engineered cells. In vivo and in vitro experiments confirmed that the scaffold could actively secrete BMP2 to significantly promote osteoblast differentiation and induce ectopic bone formation. Additionally, the scaffold exhibited broad-spectrum antibacterial capacity, thereby ensuring the survival of embedded engineered cells when facing high risk of infection. These findings demonstrated the efficacy of this bioprinted scaffold to release BMP2 in a controlled manner and prevent the occurrence of infection; thus, showing its potential for repairing infectious bone defects.
format Online
Article
Text
id pubmed-7658329
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher KeAi Publishing
record_format MEDLINE/PubMed
spelling pubmed-76583292020-11-17 A 3D-bioprinted scaffold with doxycycline-controlled BMP2-expressing cells for inducing bone regeneration and inhibiting bacterial infection Wang, Minqi Li, Hanjun Yang, Yiqi Yuan, Kai Zhou, Feng Liu, Haibei Zhou, Qinghui Yang, Shengbing Tang, Tingting Bioact Mater Article Large bone defects face a high risk of pathogen exposure due to open wounds, which leads to high infection rates and delayed bone union. To promote successful repair of infectious bone defects, fabrication of a scaffold with dual functions of osteo-induction and bacterial inhibition is required. This study describes creation of an engineered progenitor cell line (C3H10T1/2) capable of doxycycline (DOX)-mediated release of bone morphogenetic protein-2 (BMP2). Three-dimensional bioprinting technology enabled creation of scaffolds, comprising polycaprolactone/mesoporous bioactive glass/DOX and bioink, containing these engineered cells. In vivo and in vitro experiments confirmed that the scaffold could actively secrete BMP2 to significantly promote osteoblast differentiation and induce ectopic bone formation. Additionally, the scaffold exhibited broad-spectrum antibacterial capacity, thereby ensuring the survival of embedded engineered cells when facing high risk of infection. These findings demonstrated the efficacy of this bioprinted scaffold to release BMP2 in a controlled manner and prevent the occurrence of infection; thus, showing its potential for repairing infectious bone defects. KeAi Publishing 2020-11-10 /pmc/articles/PMC7658329/ /pubmed/33210025 http://dx.doi.org/10.1016/j.bioactmat.2020.10.022 Text en © 2020 [The Author/The Authors] http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Wang, Minqi
Li, Hanjun
Yang, Yiqi
Yuan, Kai
Zhou, Feng
Liu, Haibei
Zhou, Qinghui
Yang, Shengbing
Tang, Tingting
A 3D-bioprinted scaffold with doxycycline-controlled BMP2-expressing cells for inducing bone regeneration and inhibiting bacterial infection
title A 3D-bioprinted scaffold with doxycycline-controlled BMP2-expressing cells for inducing bone regeneration and inhibiting bacterial infection
title_full A 3D-bioprinted scaffold with doxycycline-controlled BMP2-expressing cells for inducing bone regeneration and inhibiting bacterial infection
title_fullStr A 3D-bioprinted scaffold with doxycycline-controlled BMP2-expressing cells for inducing bone regeneration and inhibiting bacterial infection
title_full_unstemmed A 3D-bioprinted scaffold with doxycycline-controlled BMP2-expressing cells for inducing bone regeneration and inhibiting bacterial infection
title_short A 3D-bioprinted scaffold with doxycycline-controlled BMP2-expressing cells for inducing bone regeneration and inhibiting bacterial infection
title_sort 3d-bioprinted scaffold with doxycycline-controlled bmp2-expressing cells for inducing bone regeneration and inhibiting bacterial infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7658329/
https://www.ncbi.nlm.nih.gov/pubmed/33210025
http://dx.doi.org/10.1016/j.bioactmat.2020.10.022
work_keys_str_mv AT wangminqi a3dbioprintedscaffoldwithdoxycyclinecontrolledbmp2expressingcellsforinducingboneregenerationandinhibitingbacterialinfection
AT lihanjun a3dbioprintedscaffoldwithdoxycyclinecontrolledbmp2expressingcellsforinducingboneregenerationandinhibitingbacterialinfection
AT yangyiqi a3dbioprintedscaffoldwithdoxycyclinecontrolledbmp2expressingcellsforinducingboneregenerationandinhibitingbacterialinfection
AT yuankai a3dbioprintedscaffoldwithdoxycyclinecontrolledbmp2expressingcellsforinducingboneregenerationandinhibitingbacterialinfection
AT zhoufeng a3dbioprintedscaffoldwithdoxycyclinecontrolledbmp2expressingcellsforinducingboneregenerationandinhibitingbacterialinfection
AT liuhaibei a3dbioprintedscaffoldwithdoxycyclinecontrolledbmp2expressingcellsforinducingboneregenerationandinhibitingbacterialinfection
AT zhouqinghui a3dbioprintedscaffoldwithdoxycyclinecontrolledbmp2expressingcellsforinducingboneregenerationandinhibitingbacterialinfection
AT yangshengbing a3dbioprintedscaffoldwithdoxycyclinecontrolledbmp2expressingcellsforinducingboneregenerationandinhibitingbacterialinfection
AT tangtingting a3dbioprintedscaffoldwithdoxycyclinecontrolledbmp2expressingcellsforinducingboneregenerationandinhibitingbacterialinfection
AT wangminqi 3dbioprintedscaffoldwithdoxycyclinecontrolledbmp2expressingcellsforinducingboneregenerationandinhibitingbacterialinfection
AT lihanjun 3dbioprintedscaffoldwithdoxycyclinecontrolledbmp2expressingcellsforinducingboneregenerationandinhibitingbacterialinfection
AT yangyiqi 3dbioprintedscaffoldwithdoxycyclinecontrolledbmp2expressingcellsforinducingboneregenerationandinhibitingbacterialinfection
AT yuankai 3dbioprintedscaffoldwithdoxycyclinecontrolledbmp2expressingcellsforinducingboneregenerationandinhibitingbacterialinfection
AT zhoufeng 3dbioprintedscaffoldwithdoxycyclinecontrolledbmp2expressingcellsforinducingboneregenerationandinhibitingbacterialinfection
AT liuhaibei 3dbioprintedscaffoldwithdoxycyclinecontrolledbmp2expressingcellsforinducingboneregenerationandinhibitingbacterialinfection
AT zhouqinghui 3dbioprintedscaffoldwithdoxycyclinecontrolledbmp2expressingcellsforinducingboneregenerationandinhibitingbacterialinfection
AT yangshengbing 3dbioprintedscaffoldwithdoxycyclinecontrolledbmp2expressingcellsforinducingboneregenerationandinhibitingbacterialinfection
AT tangtingting 3dbioprintedscaffoldwithdoxycyclinecontrolledbmp2expressingcellsforinducingboneregenerationandinhibitingbacterialinfection