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Surface decoration of development-inspired synthetic N-cadherin motif via Ac-BP promotes osseointegration of metal implants

Research works on the synergistic effect of surface modified bioactive molecules and bone metal implants have been highlighted. N-cadherin is regarded as a key factor in directing cell–cell interactions during the mesenchymal condensation preceding the osteogenesis in the musculoskeletal system. In...

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Detalles Bibliográficos
Autores principales: Zhu, Meiling, Zhang, Kunyu, Feng, Lu, Lin, Sien, Pan, Qi, Bian, Liming, Li, Gang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7658495/
https://www.ncbi.nlm.nih.gov/pubmed/33210028
http://dx.doi.org/10.1016/j.bioactmat.2020.11.002
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author Zhu, Meiling
Zhang, Kunyu
Feng, Lu
Lin, Sien
Pan, Qi
Bian, Liming
Li, Gang
author_facet Zhu, Meiling
Zhang, Kunyu
Feng, Lu
Lin, Sien
Pan, Qi
Bian, Liming
Li, Gang
author_sort Zhu, Meiling
collection PubMed
description Research works on the synergistic effect of surface modified bioactive molecules and bone metal implants have been highlighted. N-cadherin is regarded as a key factor in directing cell–cell interactions during the mesenchymal condensation preceding the osteogenesis in the musculoskeletal system. In this study, the N-cadherin mimetic peptide (Cad) was biofunctionalized on the titanium metal surface via the acryloyl bisphosphonate (Ac-BP). To learn the synergistic effect of N-cadherin mimetic peptide, when tethered with titanium substrates, on promoting osteogenic differentiation of the seeded human mesenchymal stem cells (hMSCs) and the osseointegration at the bone-implant interfaces. Results show that the conjugation of N-cadherin mimetic peptide with Ac-BP promoted the osteogenic gene markers expression in the hMSCs. The biofunctionalized biomaterial surfaces promote the expression of the Wnt/β-catenin downstream axis in the attached hMSCs, and then enhance the in-situ bone formation and osseointegration at the bone-implant interfaces. We conclude that this N-cadherin mimetic peptide tethered on Ti surface promote osteogenic differentiation of hMSCs and osseointegration of biomaterial implants in vitro and in vivo. These findings demonstrate the importance of the development-inspired surface bioactivation of metal implants and shed light on the possible cellular mechanisms of the enhanced osseointegration.
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spelling pubmed-76584952020-11-17 Surface decoration of development-inspired synthetic N-cadherin motif via Ac-BP promotes osseointegration of metal implants Zhu, Meiling Zhang, Kunyu Feng, Lu Lin, Sien Pan, Qi Bian, Liming Li, Gang Bioact Mater Article Research works on the synergistic effect of surface modified bioactive molecules and bone metal implants have been highlighted. N-cadherin is regarded as a key factor in directing cell–cell interactions during the mesenchymal condensation preceding the osteogenesis in the musculoskeletal system. In this study, the N-cadherin mimetic peptide (Cad) was biofunctionalized on the titanium metal surface via the acryloyl bisphosphonate (Ac-BP). To learn the synergistic effect of N-cadherin mimetic peptide, when tethered with titanium substrates, on promoting osteogenic differentiation of the seeded human mesenchymal stem cells (hMSCs) and the osseointegration at the bone-implant interfaces. Results show that the conjugation of N-cadherin mimetic peptide with Ac-BP promoted the osteogenic gene markers expression in the hMSCs. The biofunctionalized biomaterial surfaces promote the expression of the Wnt/β-catenin downstream axis in the attached hMSCs, and then enhance the in-situ bone formation and osseointegration at the bone-implant interfaces. We conclude that this N-cadherin mimetic peptide tethered on Ti surface promote osteogenic differentiation of hMSCs and osseointegration of biomaterial implants in vitro and in vivo. These findings demonstrate the importance of the development-inspired surface bioactivation of metal implants and shed light on the possible cellular mechanisms of the enhanced osseointegration. KeAi Publishing 2020-11-10 /pmc/articles/PMC7658495/ /pubmed/33210028 http://dx.doi.org/10.1016/j.bioactmat.2020.11.002 Text en © 2020 [The Author/The Authors] http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Zhu, Meiling
Zhang, Kunyu
Feng, Lu
Lin, Sien
Pan, Qi
Bian, Liming
Li, Gang
Surface decoration of development-inspired synthetic N-cadherin motif via Ac-BP promotes osseointegration of metal implants
title Surface decoration of development-inspired synthetic N-cadherin motif via Ac-BP promotes osseointegration of metal implants
title_full Surface decoration of development-inspired synthetic N-cadherin motif via Ac-BP promotes osseointegration of metal implants
title_fullStr Surface decoration of development-inspired synthetic N-cadherin motif via Ac-BP promotes osseointegration of metal implants
title_full_unstemmed Surface decoration of development-inspired synthetic N-cadherin motif via Ac-BP promotes osseointegration of metal implants
title_short Surface decoration of development-inspired synthetic N-cadherin motif via Ac-BP promotes osseointegration of metal implants
title_sort surface decoration of development-inspired synthetic n-cadherin motif via ac-bp promotes osseointegration of metal implants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7658495/
https://www.ncbi.nlm.nih.gov/pubmed/33210028
http://dx.doi.org/10.1016/j.bioactmat.2020.11.002
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