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Tanshinone IIA Inhibits Epithelial-to-Mesenchymal Transition Through Hindering β-Arrestin1 Mediated β-Catenin Signaling Pathway in Colorectal Cancer

Tanshinone IIA (Tan IIA) is a major active ingredient extracted from Salvia miltiorrhiza, which has been proved to be able to inhibit metastasis of various cancers including colorectal cancer (CRC). However, the mechanisms of anti-metastatic effect of Tan IIA on CRC are not well explored. A number o...

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Autores principales: Song, Qing, Yang, Liu, Han, Zhifen, Wu, Xinnan, Li, Ruixiao, Zhou, Lihong, Liu, Ningning, Sui, Hua, Cai, Jianfeng, Wang, Yan, Ji, Qing, Li, Qi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7658606/
https://www.ncbi.nlm.nih.gov/pubmed/33192529
http://dx.doi.org/10.3389/fphar.2020.586616
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author Song, Qing
Yang, Liu
Han, Zhifen
Wu, Xinnan
Li, Ruixiao
Zhou, Lihong
Liu, Ningning
Sui, Hua
Cai, Jianfeng
Wang, Yan
Ji, Qing
Li, Qi
author_facet Song, Qing
Yang, Liu
Han, Zhifen
Wu, Xinnan
Li, Ruixiao
Zhou, Lihong
Liu, Ningning
Sui, Hua
Cai, Jianfeng
Wang, Yan
Ji, Qing
Li, Qi
author_sort Song, Qing
collection PubMed
description Tanshinone IIA (Tan IIA) is a major active ingredient extracted from Salvia miltiorrhiza, which has been proved to be able to inhibit metastasis of various cancers including colorectal cancer (CRC). However, the mechanisms of anti-metastatic effect of Tan IIA on CRC are not well explored. A number of studies indicate that epithelial-to-mesenchymal transition (EMT) plays an important role in CRC metastasis, and our previous studies demonstrate that β-arrestin1could regulate EMT in CRC partly through β-catenin signaling pathway. In this work, we investigate whether Tan IIA could regulate EMT in CRC through β-arrestin1-mediated β-catenin signaling pathway both in vivo and in vitro. Our results showed that Tan IIA inhibited lung metastases of CRC cells in vivo and extended the survival time of mice with CRC. In vitro, Tan IIA increased the expression of E-cadherin, decreased the expression of Snail, N-cadherin and Vimentin, thus suppressed EMT and the migratory ability of CRC cells. Further study found that the mechanism of action of Tan IIA in regulating EMT and metastasis is associated with the suppression of β-arrestin1 expression, resulting in the increase of GSK-3β expression, reduction of β-catenin nuclear localization, thereby decreased the activity of β-catenin signaling pathway. Our data revealed a new mechanism of Tan IIA on the suppression of EMT and metastasis in CRC via β-arrestin1-mediated β-catenin signaling pathway and provided support for using Tan IIA as anti-metastatic agents in CRC.
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spelling pubmed-76586062020-11-13 Tanshinone IIA Inhibits Epithelial-to-Mesenchymal Transition Through Hindering β-Arrestin1 Mediated β-Catenin Signaling Pathway in Colorectal Cancer Song, Qing Yang, Liu Han, Zhifen Wu, Xinnan Li, Ruixiao Zhou, Lihong Liu, Ningning Sui, Hua Cai, Jianfeng Wang, Yan Ji, Qing Li, Qi Front Pharmacol Pharmacology Tanshinone IIA (Tan IIA) is a major active ingredient extracted from Salvia miltiorrhiza, which has been proved to be able to inhibit metastasis of various cancers including colorectal cancer (CRC). However, the mechanisms of anti-metastatic effect of Tan IIA on CRC are not well explored. A number of studies indicate that epithelial-to-mesenchymal transition (EMT) plays an important role in CRC metastasis, and our previous studies demonstrate that β-arrestin1could regulate EMT in CRC partly through β-catenin signaling pathway. In this work, we investigate whether Tan IIA could regulate EMT in CRC through β-arrestin1-mediated β-catenin signaling pathway both in vivo and in vitro. Our results showed that Tan IIA inhibited lung metastases of CRC cells in vivo and extended the survival time of mice with CRC. In vitro, Tan IIA increased the expression of E-cadherin, decreased the expression of Snail, N-cadherin and Vimentin, thus suppressed EMT and the migratory ability of CRC cells. Further study found that the mechanism of action of Tan IIA in regulating EMT and metastasis is associated with the suppression of β-arrestin1 expression, resulting in the increase of GSK-3β expression, reduction of β-catenin nuclear localization, thereby decreased the activity of β-catenin signaling pathway. Our data revealed a new mechanism of Tan IIA on the suppression of EMT and metastasis in CRC via β-arrestin1-mediated β-catenin signaling pathway and provided support for using Tan IIA as anti-metastatic agents in CRC. Frontiers Media S.A. 2020-10-29 /pmc/articles/PMC7658606/ /pubmed/33192529 http://dx.doi.org/10.3389/fphar.2020.586616 Text en Copyright © 2020 Song, Yang, Han, Wu, Li, Zhou, Liu, Sui, Cai, Wang, Ji and Qi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Song, Qing
Yang, Liu
Han, Zhifen
Wu, Xinnan
Li, Ruixiao
Zhou, Lihong
Liu, Ningning
Sui, Hua
Cai, Jianfeng
Wang, Yan
Ji, Qing
Li, Qi
Tanshinone IIA Inhibits Epithelial-to-Mesenchymal Transition Through Hindering β-Arrestin1 Mediated β-Catenin Signaling Pathway in Colorectal Cancer
title Tanshinone IIA Inhibits Epithelial-to-Mesenchymal Transition Through Hindering β-Arrestin1 Mediated β-Catenin Signaling Pathway in Colorectal Cancer
title_full Tanshinone IIA Inhibits Epithelial-to-Mesenchymal Transition Through Hindering β-Arrestin1 Mediated β-Catenin Signaling Pathway in Colorectal Cancer
title_fullStr Tanshinone IIA Inhibits Epithelial-to-Mesenchymal Transition Through Hindering β-Arrestin1 Mediated β-Catenin Signaling Pathway in Colorectal Cancer
title_full_unstemmed Tanshinone IIA Inhibits Epithelial-to-Mesenchymal Transition Through Hindering β-Arrestin1 Mediated β-Catenin Signaling Pathway in Colorectal Cancer
title_short Tanshinone IIA Inhibits Epithelial-to-Mesenchymal Transition Through Hindering β-Arrestin1 Mediated β-Catenin Signaling Pathway in Colorectal Cancer
title_sort tanshinone iia inhibits epithelial-to-mesenchymal transition through hindering β-arrestin1 mediated β-catenin signaling pathway in colorectal cancer
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7658606/
https://www.ncbi.nlm.nih.gov/pubmed/33192529
http://dx.doi.org/10.3389/fphar.2020.586616
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