Short DNA Oligonucleotide as a Ag(+) Binding Detector

[Image: see text] Ag(+) has been known to mediate several natural metallo-base pairs. Based on the unique structural information of a short 8-mer DNA strand (5′-GCACGCGC-3′) induced by Ag(+), we constructed several fluorescent DNA beacons for the detection of Ag(+) according to the increase in the f...

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Detalles Bibliográficos
Autores principales: Shen, Fusheng, Mao, Song, Mathivanan, Johnsi, Wu, Ying, Chandrasekaran, Arun Richard, Liu, Hehua, Gan, Jianhua, Sheng, Jia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7658945/
https://www.ncbi.nlm.nih.gov/pubmed/33195907
http://dx.doi.org/10.1021/acsomega.0c03372
Descripción
Sumario:[Image: see text] Ag(+) has been known to mediate several natural metallo-base pairs. Based on the unique structural information of a short 8-mer DNA strand (5′-GCACGCGC-3′) induced by Ag(+), we constructed several fluorescent DNA beacons for the detection of Ag(+) according to the increase in the fluorescence emission on Ag(+) binding. This Ag(+) detection assay is quick, sensitive, and easy to adapt and can function in a wide range of temperatures from 5 to 65 °C.

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