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Conversion of poplar biomass into high-energy density tricyclic sesquiterpene jet fuel blendstocks

BACKGROUND: In an effort to ensure future energy security, reduce greenhouse gas emissions and create domestic jobs, the US has invested in technologies to develop sustainable biofuels and bioproducts from renewable carbon sources such as lignocellulosic biomass. Bio-derived jet fuel is of particula...

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Autores principales: Geiselman, Gina M., Kirby, James, Landera, Alexander, Otoupal, Peter, Papa, Gabriella, Barcelos, Carolina, Sundstrom, Eric R., Das, Lalitendu, Magurudeniya, Harsha D., Wehrs, Maren, Rodriguez, Alberto, Simmons, Blake A., Magnuson, Jon K., Mukhopadhyay, Aindrila, Lee, Taek Soon, George, Anthe, Gladden, John M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7659065/
https://www.ncbi.nlm.nih.gov/pubmed/33183275
http://dx.doi.org/10.1186/s12934-020-01456-4
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author Geiselman, Gina M.
Kirby, James
Landera, Alexander
Otoupal, Peter
Papa, Gabriella
Barcelos, Carolina
Sundstrom, Eric R.
Das, Lalitendu
Magurudeniya, Harsha D.
Wehrs, Maren
Rodriguez, Alberto
Simmons, Blake A.
Magnuson, Jon K.
Mukhopadhyay, Aindrila
Lee, Taek Soon
George, Anthe
Gladden, John M.
author_facet Geiselman, Gina M.
Kirby, James
Landera, Alexander
Otoupal, Peter
Papa, Gabriella
Barcelos, Carolina
Sundstrom, Eric R.
Das, Lalitendu
Magurudeniya, Harsha D.
Wehrs, Maren
Rodriguez, Alberto
Simmons, Blake A.
Magnuson, Jon K.
Mukhopadhyay, Aindrila
Lee, Taek Soon
George, Anthe
Gladden, John M.
author_sort Geiselman, Gina M.
collection PubMed
description BACKGROUND: In an effort to ensure future energy security, reduce greenhouse gas emissions and create domestic jobs, the US has invested in technologies to develop sustainable biofuels and bioproducts from renewable carbon sources such as lignocellulosic biomass. Bio-derived jet fuel is of particular interest as aviation is less amenable to electrification compared to other modes of transportation and synthetic biology provides the ability to tailor fuel properties to enhance performance. Specific energy and energy density are important properties in determining the attractiveness of potential bio-derived jet fuels. For example, increased energy content can give the industry options such as longer range, higher load or reduced takeoff weight. Energy-dense sesquiterpenes have been identified as potential next-generation jet fuels that can be renewably produced from lignocellulosic biomass. RESULTS: We developed a biomass deconstruction and conversion process that enabled the production of two tricyclic sesquiterpenes, epi-isozizaene and prespatane, from the woody biomass poplar using the versatile basidiomycete Rhodosporidium toruloides. We demonstrated terpene production at both bench and bioreactor scales, with prespatane titers reaching 1173.6 mg/L when grown in poplar hydrolysate in a 2 L bioreactor. Additionally, we examined the theoretical fuel properties of prespatane and epi-isozizaene in their hydrogenated states as blending options for jet fuel, and compared them to aviation fuel, Jet A. CONCLUSION: Our findings indicate that prespatane and epi-isozizaene in their hydrogenated states would be attractive blending options in Jet A or other lower density renewable jet fuels as they would improve viscosity and increase their energy density. Saturated epi-isozizaene and saturated prespatane have energy densities that are 16.6 and 18.8% higher than Jet A, respectively. These results highlight the potential of R. toruloides as a production host for the sustainable and scalable production of bio-derived jet fuel blends, and this is the first report of prespatane as an alternative jet fuel.
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spelling pubmed-76590652020-11-13 Conversion of poplar biomass into high-energy density tricyclic sesquiterpene jet fuel blendstocks Geiselman, Gina M. Kirby, James Landera, Alexander Otoupal, Peter Papa, Gabriella Barcelos, Carolina Sundstrom, Eric R. Das, Lalitendu Magurudeniya, Harsha D. Wehrs, Maren Rodriguez, Alberto Simmons, Blake A. Magnuson, Jon K. Mukhopadhyay, Aindrila Lee, Taek Soon George, Anthe Gladden, John M. Microb Cell Fact Research BACKGROUND: In an effort to ensure future energy security, reduce greenhouse gas emissions and create domestic jobs, the US has invested in technologies to develop sustainable biofuels and bioproducts from renewable carbon sources such as lignocellulosic biomass. Bio-derived jet fuel is of particular interest as aviation is less amenable to electrification compared to other modes of transportation and synthetic biology provides the ability to tailor fuel properties to enhance performance. Specific energy and energy density are important properties in determining the attractiveness of potential bio-derived jet fuels. For example, increased energy content can give the industry options such as longer range, higher load or reduced takeoff weight. Energy-dense sesquiterpenes have been identified as potential next-generation jet fuels that can be renewably produced from lignocellulosic biomass. RESULTS: We developed a biomass deconstruction and conversion process that enabled the production of two tricyclic sesquiterpenes, epi-isozizaene and prespatane, from the woody biomass poplar using the versatile basidiomycete Rhodosporidium toruloides. We demonstrated terpene production at both bench and bioreactor scales, with prespatane titers reaching 1173.6 mg/L when grown in poplar hydrolysate in a 2 L bioreactor. Additionally, we examined the theoretical fuel properties of prespatane and epi-isozizaene in their hydrogenated states as blending options for jet fuel, and compared them to aviation fuel, Jet A. CONCLUSION: Our findings indicate that prespatane and epi-isozizaene in their hydrogenated states would be attractive blending options in Jet A or other lower density renewable jet fuels as they would improve viscosity and increase their energy density. Saturated epi-isozizaene and saturated prespatane have energy densities that are 16.6 and 18.8% higher than Jet A, respectively. These results highlight the potential of R. toruloides as a production host for the sustainable and scalable production of bio-derived jet fuel blends, and this is the first report of prespatane as an alternative jet fuel. BioMed Central 2020-11-12 /pmc/articles/PMC7659065/ /pubmed/33183275 http://dx.doi.org/10.1186/s12934-020-01456-4 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Geiselman, Gina M.
Kirby, James
Landera, Alexander
Otoupal, Peter
Papa, Gabriella
Barcelos, Carolina
Sundstrom, Eric R.
Das, Lalitendu
Magurudeniya, Harsha D.
Wehrs, Maren
Rodriguez, Alberto
Simmons, Blake A.
Magnuson, Jon K.
Mukhopadhyay, Aindrila
Lee, Taek Soon
George, Anthe
Gladden, John M.
Conversion of poplar biomass into high-energy density tricyclic sesquiterpene jet fuel blendstocks
title Conversion of poplar biomass into high-energy density tricyclic sesquiterpene jet fuel blendstocks
title_full Conversion of poplar biomass into high-energy density tricyclic sesquiterpene jet fuel blendstocks
title_fullStr Conversion of poplar biomass into high-energy density tricyclic sesquiterpene jet fuel blendstocks
title_full_unstemmed Conversion of poplar biomass into high-energy density tricyclic sesquiterpene jet fuel blendstocks
title_short Conversion of poplar biomass into high-energy density tricyclic sesquiterpene jet fuel blendstocks
title_sort conversion of poplar biomass into high-energy density tricyclic sesquiterpene jet fuel blendstocks
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7659065/
https://www.ncbi.nlm.nih.gov/pubmed/33183275
http://dx.doi.org/10.1186/s12934-020-01456-4
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