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Viral rhodopsins 1 are an unique family of light-gated cation channels

Phytoplankton is the base of the marine food chain as well as oxygen and carbon cycles and thus plays a global role in climate and ecology. Nucleocytoplasmic Large DNA Viruses that infect phytoplankton organisms and regulate the phytoplankton dynamics encompass genes of rhodopsins of two distinct fa...

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Autores principales: Zabelskii, Dmitrii, Alekseev, Alexey, Kovalev, Kirill, Rankovic, Vladan, Balandin, Taras, Soloviov, Dmytro, Bratanov, Dmitry, Savelyeva, Ekaterina, Podolyak, Elizaveta, Volkov, Dmytro, Vaganova, Svetlana, Astashkin, Roman, Chizhov, Igor, Yutin, Natalia, Rulev, Maksim, Popov, Alexander, Eria-Oliveira, Ana-Sofia, Rokitskaya, Tatiana, Mager, Thomas, Antonenko, Yuri, Rosselli, Riccardo, Armeev, Grigoriy, Shaitan, Konstantin, Vivaudou, Michel, Büldt, Georg, Rogachev, Andrey, Rodriguez-Valera, Francisco, Kirpichnikov, Mikhail, Moser, Tobias, Offenhäusser, Andreas, Willbold, Dieter, Koonin, Eugene, Bamberg, Ernst, Gordeliy, Valentin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7659345/
https://www.ncbi.nlm.nih.gov/pubmed/33177509
http://dx.doi.org/10.1038/s41467-020-19457-7
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author Zabelskii, Dmitrii
Alekseev, Alexey
Kovalev, Kirill
Rankovic, Vladan
Balandin, Taras
Soloviov, Dmytro
Bratanov, Dmitry
Savelyeva, Ekaterina
Podolyak, Elizaveta
Volkov, Dmytro
Vaganova, Svetlana
Astashkin, Roman
Chizhov, Igor
Yutin, Natalia
Rulev, Maksim
Popov, Alexander
Eria-Oliveira, Ana-Sofia
Rokitskaya, Tatiana
Mager, Thomas
Antonenko, Yuri
Rosselli, Riccardo
Armeev, Grigoriy
Shaitan, Konstantin
Vivaudou, Michel
Büldt, Georg
Rogachev, Andrey
Rodriguez-Valera, Francisco
Kirpichnikov, Mikhail
Moser, Tobias
Offenhäusser, Andreas
Willbold, Dieter
Koonin, Eugene
Bamberg, Ernst
Gordeliy, Valentin
author_facet Zabelskii, Dmitrii
Alekseev, Alexey
Kovalev, Kirill
Rankovic, Vladan
Balandin, Taras
Soloviov, Dmytro
Bratanov, Dmitry
Savelyeva, Ekaterina
Podolyak, Elizaveta
Volkov, Dmytro
Vaganova, Svetlana
Astashkin, Roman
Chizhov, Igor
Yutin, Natalia
Rulev, Maksim
Popov, Alexander
Eria-Oliveira, Ana-Sofia
Rokitskaya, Tatiana
Mager, Thomas
Antonenko, Yuri
Rosselli, Riccardo
Armeev, Grigoriy
Shaitan, Konstantin
Vivaudou, Michel
Büldt, Georg
Rogachev, Andrey
Rodriguez-Valera, Francisco
Kirpichnikov, Mikhail
Moser, Tobias
Offenhäusser, Andreas
Willbold, Dieter
Koonin, Eugene
Bamberg, Ernst
Gordeliy, Valentin
author_sort Zabelskii, Dmitrii
collection PubMed
description Phytoplankton is the base of the marine food chain as well as oxygen and carbon cycles and thus plays a global role in climate and ecology. Nucleocytoplasmic Large DNA Viruses that infect phytoplankton organisms and regulate the phytoplankton dynamics encompass genes of rhodopsins of two distinct families. Here, we present a functional and structural characterization of two proteins of viral rhodopsin group 1, OLPVR1 and VirChR1. Functional analysis of VirChR1 shows that it is a highly selective, Na(+)/K(+)-conducting channel and, in contrast to known cation channelrhodopsins, it is impermeable to Ca(2+) ions. We show that, upon illumination, VirChR1 is able to drive neural firing. The 1.4 Å resolution structure of OLPVR1 reveals remarkable differences from the known channelrhodopsins and a unique ion-conducting pathway. Thus, viral rhodopsins 1 represent a unique, large group of light-gated channels (viral channelrhodopsins, VirChR1s). In nature, VirChR1s likely mediate phototaxis of algae enhancing the host anabolic processes to support virus reproduction, and therefore, might play a major role in global phytoplankton dynamics. Moreover, VirChR1s have unique potential for optogenetics as they lack possibly noxious Ca(2+) permeability.
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spelling pubmed-76593452020-11-17 Viral rhodopsins 1 are an unique family of light-gated cation channels Zabelskii, Dmitrii Alekseev, Alexey Kovalev, Kirill Rankovic, Vladan Balandin, Taras Soloviov, Dmytro Bratanov, Dmitry Savelyeva, Ekaterina Podolyak, Elizaveta Volkov, Dmytro Vaganova, Svetlana Astashkin, Roman Chizhov, Igor Yutin, Natalia Rulev, Maksim Popov, Alexander Eria-Oliveira, Ana-Sofia Rokitskaya, Tatiana Mager, Thomas Antonenko, Yuri Rosselli, Riccardo Armeev, Grigoriy Shaitan, Konstantin Vivaudou, Michel Büldt, Georg Rogachev, Andrey Rodriguez-Valera, Francisco Kirpichnikov, Mikhail Moser, Tobias Offenhäusser, Andreas Willbold, Dieter Koonin, Eugene Bamberg, Ernst Gordeliy, Valentin Nat Commun Article Phytoplankton is the base of the marine food chain as well as oxygen and carbon cycles and thus plays a global role in climate and ecology. Nucleocytoplasmic Large DNA Viruses that infect phytoplankton organisms and regulate the phytoplankton dynamics encompass genes of rhodopsins of two distinct families. Here, we present a functional and structural characterization of two proteins of viral rhodopsin group 1, OLPVR1 and VirChR1. Functional analysis of VirChR1 shows that it is a highly selective, Na(+)/K(+)-conducting channel and, in contrast to known cation channelrhodopsins, it is impermeable to Ca(2+) ions. We show that, upon illumination, VirChR1 is able to drive neural firing. The 1.4 Å resolution structure of OLPVR1 reveals remarkable differences from the known channelrhodopsins and a unique ion-conducting pathway. Thus, viral rhodopsins 1 represent a unique, large group of light-gated channels (viral channelrhodopsins, VirChR1s). In nature, VirChR1s likely mediate phototaxis of algae enhancing the host anabolic processes to support virus reproduction, and therefore, might play a major role in global phytoplankton dynamics. Moreover, VirChR1s have unique potential for optogenetics as they lack possibly noxious Ca(2+) permeability. Nature Publishing Group UK 2020-11-11 /pmc/articles/PMC7659345/ /pubmed/33177509 http://dx.doi.org/10.1038/s41467-020-19457-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zabelskii, Dmitrii
Alekseev, Alexey
Kovalev, Kirill
Rankovic, Vladan
Balandin, Taras
Soloviov, Dmytro
Bratanov, Dmitry
Savelyeva, Ekaterina
Podolyak, Elizaveta
Volkov, Dmytro
Vaganova, Svetlana
Astashkin, Roman
Chizhov, Igor
Yutin, Natalia
Rulev, Maksim
Popov, Alexander
Eria-Oliveira, Ana-Sofia
Rokitskaya, Tatiana
Mager, Thomas
Antonenko, Yuri
Rosselli, Riccardo
Armeev, Grigoriy
Shaitan, Konstantin
Vivaudou, Michel
Büldt, Georg
Rogachev, Andrey
Rodriguez-Valera, Francisco
Kirpichnikov, Mikhail
Moser, Tobias
Offenhäusser, Andreas
Willbold, Dieter
Koonin, Eugene
Bamberg, Ernst
Gordeliy, Valentin
Viral rhodopsins 1 are an unique family of light-gated cation channels
title Viral rhodopsins 1 are an unique family of light-gated cation channels
title_full Viral rhodopsins 1 are an unique family of light-gated cation channels
title_fullStr Viral rhodopsins 1 are an unique family of light-gated cation channels
title_full_unstemmed Viral rhodopsins 1 are an unique family of light-gated cation channels
title_short Viral rhodopsins 1 are an unique family of light-gated cation channels
title_sort viral rhodopsins 1 are an unique family of light-gated cation channels
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7659345/
https://www.ncbi.nlm.nih.gov/pubmed/33177509
http://dx.doi.org/10.1038/s41467-020-19457-7
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