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Impact of DBBM Fragments on the Porosity of the Calvarial Bone: A Pilot Study on Mice

Deproteinized bovine bone mineral (DBBM) is brittle and can break into fragments. Here, we examined whether DBBM fragments have an impact on mice calvarial bone during bone augmentation. DBBM was either randomly crushed (DBBM fragments) or left undisturbed (DBBM granules). Then, DBBM fragments or or...

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Autores principales: Kuchler, Ulrike, Heimel, Patrick, Stähli, Alexandra, Josef Strauss, Franz, Luza, Bernadette, Gruber, Reinhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7660694/
https://www.ncbi.nlm.nih.gov/pubmed/33114211
http://dx.doi.org/10.3390/ma13214748
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author Kuchler, Ulrike
Heimel, Patrick
Stähli, Alexandra
Josef Strauss, Franz
Luza, Bernadette
Gruber, Reinhard
author_facet Kuchler, Ulrike
Heimel, Patrick
Stähli, Alexandra
Josef Strauss, Franz
Luza, Bernadette
Gruber, Reinhard
author_sort Kuchler, Ulrike
collection PubMed
description Deproteinized bovine bone mineral (DBBM) is brittle and can break into fragments. Here, we examined whether DBBM fragments have an impact on mice calvarial bone during bone augmentation. DBBM was either randomly crushed (DBBM fragments) or left undisturbed (DBBM granules). Then, DBBM fragments or original DBBM granules were placed onto calvarial bone in 20 BALB/c mice. Following random allocation, ten mice received DBBM fragments and ten mice received original DBBM granules. After fourteen days of healing, micro computed tomography (micro-CT) and histological analysis of the augmented sites were performed. The primary outcome was the porosity of the calvarial bone. The micro-CT analysis revealed that DBBM fragments failed to significantly change the porosity of the calvarial bone as compared with original DBBM granules, despite the slightly higher bone resorption in the DBBM fragment group, 10.3% (CI 6.3–11.6) versus 6.1% (CI 4.1–7.8, p = 0.355), respectively. The cortical bone volume was not altered by DBBM fragments as compared with original DBBM granules, i.e., 79.0% (CI 78.9–81.2) versus 81.5% (CI 80.1–83.3, p = 0.357), respectively. The DBBM fragment group revealed similar bone thickness values as compared with the DBBM granules group, i.e., 0.26 mm (CI 0.23–0.29) versus 0.25 mm (CI 0.22–0.27, p = 0.641), respectively. The histological evaluation supported the micro-CT observations, displaying minor signs of porosity and resorption. The particle-size distribution analysis confirmed a shift towards smaller particle sizes in the DBBM fragment group. These findings suggest that DBBM fragments behave similarly to original DBBM granules in terms of bone morphological changes at augmented sites.
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spelling pubmed-76606942020-11-13 Impact of DBBM Fragments on the Porosity of the Calvarial Bone: A Pilot Study on Mice Kuchler, Ulrike Heimel, Patrick Stähli, Alexandra Josef Strauss, Franz Luza, Bernadette Gruber, Reinhard Materials (Basel) Article Deproteinized bovine bone mineral (DBBM) is brittle and can break into fragments. Here, we examined whether DBBM fragments have an impact on mice calvarial bone during bone augmentation. DBBM was either randomly crushed (DBBM fragments) or left undisturbed (DBBM granules). Then, DBBM fragments or original DBBM granules were placed onto calvarial bone in 20 BALB/c mice. Following random allocation, ten mice received DBBM fragments and ten mice received original DBBM granules. After fourteen days of healing, micro computed tomography (micro-CT) and histological analysis of the augmented sites were performed. The primary outcome was the porosity of the calvarial bone. The micro-CT analysis revealed that DBBM fragments failed to significantly change the porosity of the calvarial bone as compared with original DBBM granules, despite the slightly higher bone resorption in the DBBM fragment group, 10.3% (CI 6.3–11.6) versus 6.1% (CI 4.1–7.8, p = 0.355), respectively. The cortical bone volume was not altered by DBBM fragments as compared with original DBBM granules, i.e., 79.0% (CI 78.9–81.2) versus 81.5% (CI 80.1–83.3, p = 0.357), respectively. The DBBM fragment group revealed similar bone thickness values as compared with the DBBM granules group, i.e., 0.26 mm (CI 0.23–0.29) versus 0.25 mm (CI 0.22–0.27, p = 0.641), respectively. The histological evaluation supported the micro-CT observations, displaying minor signs of porosity and resorption. The particle-size distribution analysis confirmed a shift towards smaller particle sizes in the DBBM fragment group. These findings suggest that DBBM fragments behave similarly to original DBBM granules in terms of bone morphological changes at augmented sites. MDPI 2020-10-23 /pmc/articles/PMC7660694/ /pubmed/33114211 http://dx.doi.org/10.3390/ma13214748 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kuchler, Ulrike
Heimel, Patrick
Stähli, Alexandra
Josef Strauss, Franz
Luza, Bernadette
Gruber, Reinhard
Impact of DBBM Fragments on the Porosity of the Calvarial Bone: A Pilot Study on Mice
title Impact of DBBM Fragments on the Porosity of the Calvarial Bone: A Pilot Study on Mice
title_full Impact of DBBM Fragments on the Porosity of the Calvarial Bone: A Pilot Study on Mice
title_fullStr Impact of DBBM Fragments on the Porosity of the Calvarial Bone: A Pilot Study on Mice
title_full_unstemmed Impact of DBBM Fragments on the Porosity of the Calvarial Bone: A Pilot Study on Mice
title_short Impact of DBBM Fragments on the Porosity of the Calvarial Bone: A Pilot Study on Mice
title_sort impact of dbbm fragments on the porosity of the calvarial bone: a pilot study on mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7660694/
https://www.ncbi.nlm.nih.gov/pubmed/33114211
http://dx.doi.org/10.3390/ma13214748
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