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Correlations of Calf Muscle Macrophage Content With Muscle Properties and Walking Performance in Peripheral Artery Disease

BACKGROUND: Peripheral artery disease (PAD) is a manifestation of atherosclerosis characterized by reduced blood flow to the lower extremities and mobility loss. Preliminary evidence suggests PAD damages skeletal muscle, resulting in muscle impairments that contribute to functional decline. We sough...

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Autores principales: Kosmac, Kate, Gonzalez‐Freire, Marta, McDermott, Mary M., White, Sarah H., Walton, R. Grace, Sufit, Robert L., Tian, Lu, Li, Lingyu, Kibbe, Melina R., Criqui, Michael H., Guralnik, Jack M., S. Polonsky, Tamar, Leeuwenburgh, Christiaan, Ferrucci, Luigi, Peterson, Charlotte A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7660852/
https://www.ncbi.nlm.nih.gov/pubmed/32390569
http://dx.doi.org/10.1161/JAHA.118.015929
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author Kosmac, Kate
Gonzalez‐Freire, Marta
McDermott, Mary M.
White, Sarah H.
Walton, R. Grace
Sufit, Robert L.
Tian, Lu
Li, Lingyu
Kibbe, Melina R.
Criqui, Michael H.
Guralnik, Jack M.
S. Polonsky, Tamar
Leeuwenburgh, Christiaan
Ferrucci, Luigi
Peterson, Charlotte A.
author_facet Kosmac, Kate
Gonzalez‐Freire, Marta
McDermott, Mary M.
White, Sarah H.
Walton, R. Grace
Sufit, Robert L.
Tian, Lu
Li, Lingyu
Kibbe, Melina R.
Criqui, Michael H.
Guralnik, Jack M.
S. Polonsky, Tamar
Leeuwenburgh, Christiaan
Ferrucci, Luigi
Peterson, Charlotte A.
author_sort Kosmac, Kate
collection PubMed
description BACKGROUND: Peripheral artery disease (PAD) is a manifestation of atherosclerosis characterized by reduced blood flow to the lower extremities and mobility loss. Preliminary evidence suggests PAD damages skeletal muscle, resulting in muscle impairments that contribute to functional decline. We sought to determine whether PAD is associated with an altered macrophage profile in gastrocnemius muscles and whether muscle macrophage populations are associated with impaired muscle phenotype and walking performance in patients with PAD. METHODS AND RESULTS: Macrophages, satellite cells, and extracellular matrix in gastrocnemius muscles from 25 patients with PAD and 7 patients without PAD were quantified using immunohistochemistry. Among patients with PAD, both the absolute number and percentage of cluster of differentiation (CD) 11b+CD206+ M2‐like macrophages positively correlated to satellite cell number (r=0.461 [P=0.023] and r=0.416 [P=0.042], respectively) but not capillary density or extracellular matrix. The number of CD11b+CD206− macrophages negatively correlated to 4‐meter walk tests at normal (r=−0.447, P=0.036) and fast pace (r=−0.510, P=0.014). Extracellular matrix occupied more muscle area in PAD compared with non‐PAD (8.72±2.19% versus 5.30±1.03%, P<0.001) and positively correlated with capillary density (r=0.656, P<0.001). CONCLUSIONS: Among people with PAD, higher CD206+ M2‐like macrophage abundance was associated with greater satellite cell numbers and muscle fiber size. Lower CD206− macrophage abundance was associated with better walking performance. Further study is needed to determine whether CD206+ macrophages are associated with ongoing reparative processes enabling skeletal muscle adaptation to damage with PAD. REGISTRATION: URL: https://www.clini​caltr​ials.gov; Unique identifiers: NCT00693940, NCT01408901, NCT0224660.
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spelling pubmed-76608522020-11-17 Correlations of Calf Muscle Macrophage Content With Muscle Properties and Walking Performance in Peripheral Artery Disease Kosmac, Kate Gonzalez‐Freire, Marta McDermott, Mary M. White, Sarah H. Walton, R. Grace Sufit, Robert L. Tian, Lu Li, Lingyu Kibbe, Melina R. Criqui, Michael H. Guralnik, Jack M. S. Polonsky, Tamar Leeuwenburgh, Christiaan Ferrucci, Luigi Peterson, Charlotte A. J Am Heart Assoc Original Research BACKGROUND: Peripheral artery disease (PAD) is a manifestation of atherosclerosis characterized by reduced blood flow to the lower extremities and mobility loss. Preliminary evidence suggests PAD damages skeletal muscle, resulting in muscle impairments that contribute to functional decline. We sought to determine whether PAD is associated with an altered macrophage profile in gastrocnemius muscles and whether muscle macrophage populations are associated with impaired muscle phenotype and walking performance in patients with PAD. METHODS AND RESULTS: Macrophages, satellite cells, and extracellular matrix in gastrocnemius muscles from 25 patients with PAD and 7 patients without PAD were quantified using immunohistochemistry. Among patients with PAD, both the absolute number and percentage of cluster of differentiation (CD) 11b+CD206+ M2‐like macrophages positively correlated to satellite cell number (r=0.461 [P=0.023] and r=0.416 [P=0.042], respectively) but not capillary density or extracellular matrix. The number of CD11b+CD206− macrophages negatively correlated to 4‐meter walk tests at normal (r=−0.447, P=0.036) and fast pace (r=−0.510, P=0.014). Extracellular matrix occupied more muscle area in PAD compared with non‐PAD (8.72±2.19% versus 5.30±1.03%, P<0.001) and positively correlated with capillary density (r=0.656, P<0.001). CONCLUSIONS: Among people with PAD, higher CD206+ M2‐like macrophage abundance was associated with greater satellite cell numbers and muscle fiber size. Lower CD206− macrophage abundance was associated with better walking performance. Further study is needed to determine whether CD206+ macrophages are associated with ongoing reparative processes enabling skeletal muscle adaptation to damage with PAD. REGISTRATION: URL: https://www.clini​caltr​ials.gov; Unique identifiers: NCT00693940, NCT01408901, NCT0224660. John Wiley and Sons Inc. 2020-05-09 /pmc/articles/PMC7660852/ /pubmed/32390569 http://dx.doi.org/10.1161/JAHA.118.015929 Text en © 2020 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Research
Kosmac, Kate
Gonzalez‐Freire, Marta
McDermott, Mary M.
White, Sarah H.
Walton, R. Grace
Sufit, Robert L.
Tian, Lu
Li, Lingyu
Kibbe, Melina R.
Criqui, Michael H.
Guralnik, Jack M.
S. Polonsky, Tamar
Leeuwenburgh, Christiaan
Ferrucci, Luigi
Peterson, Charlotte A.
Correlations of Calf Muscle Macrophage Content With Muscle Properties and Walking Performance in Peripheral Artery Disease
title Correlations of Calf Muscle Macrophage Content With Muscle Properties and Walking Performance in Peripheral Artery Disease
title_full Correlations of Calf Muscle Macrophage Content With Muscle Properties and Walking Performance in Peripheral Artery Disease
title_fullStr Correlations of Calf Muscle Macrophage Content With Muscle Properties and Walking Performance in Peripheral Artery Disease
title_full_unstemmed Correlations of Calf Muscle Macrophage Content With Muscle Properties and Walking Performance in Peripheral Artery Disease
title_short Correlations of Calf Muscle Macrophage Content With Muscle Properties and Walking Performance in Peripheral Artery Disease
title_sort correlations of calf muscle macrophage content with muscle properties and walking performance in peripheral artery disease
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7660852/
https://www.ncbi.nlm.nih.gov/pubmed/32390569
http://dx.doi.org/10.1161/JAHA.118.015929
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