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Genetic engineering of sex chromosomes for batch cultivation of non-transgenic, sex-sorted males
The field performance of Sterile Insect Technique (SIT) is improved by sex-sorting and releasing only sterile males. This can be accomplished by resource-intensive separation of males from females by morphology. Alternatively, sex-ratio biasing genetic constructs can be used to selectively remove on...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7660900/ https://www.ncbi.nlm.nih.gov/pubmed/33137115 http://dx.doi.org/10.1371/journal.pgen.1009180 |
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author | Das, Siba R. Maselko, Maciej Upadhyay, Ambuj Smanski, Michael J. |
author_facet | Das, Siba R. Maselko, Maciej Upadhyay, Ambuj Smanski, Michael J. |
author_sort | Das, Siba R. |
collection | PubMed |
description | The field performance of Sterile Insect Technique (SIT) is improved by sex-sorting and releasing only sterile males. This can be accomplished by resource-intensive separation of males from females by morphology. Alternatively, sex-ratio biasing genetic constructs can be used to selectively remove one sex without the need for manual or automated sorting, but the resulting genetically engineered (GE) control agents would be subject to additional governmental regulation. Here we describe and demonstrate a genetic method for the batch production of non-GE males. This method could be applied to generate the heterogametic sex (XY, or WZ) in any organism with chromosomal sex determination. We observed up to 100% sex-selection with batch cultures of more than 10(3) individuals. Using a stringent transgene detection assay, we demonstrate the potential of mass production of transgene free males. |
format | Online Article Text |
id | pubmed-7660900 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-76609002020-11-18 Genetic engineering of sex chromosomes for batch cultivation of non-transgenic, sex-sorted males Das, Siba R. Maselko, Maciej Upadhyay, Ambuj Smanski, Michael J. PLoS Genet Research Article The field performance of Sterile Insect Technique (SIT) is improved by sex-sorting and releasing only sterile males. This can be accomplished by resource-intensive separation of males from females by morphology. Alternatively, sex-ratio biasing genetic constructs can be used to selectively remove one sex without the need for manual or automated sorting, but the resulting genetically engineered (GE) control agents would be subject to additional governmental regulation. Here we describe and demonstrate a genetic method for the batch production of non-GE males. This method could be applied to generate the heterogametic sex (XY, or WZ) in any organism with chromosomal sex determination. We observed up to 100% sex-selection with batch cultures of more than 10(3) individuals. Using a stringent transgene detection assay, we demonstrate the potential of mass production of transgene free males. Public Library of Science 2020-11-02 /pmc/articles/PMC7660900/ /pubmed/33137115 http://dx.doi.org/10.1371/journal.pgen.1009180 Text en © 2020 Das et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Das, Siba R. Maselko, Maciej Upadhyay, Ambuj Smanski, Michael J. Genetic engineering of sex chromosomes for batch cultivation of non-transgenic, sex-sorted males |
title | Genetic engineering of sex chromosomes for batch cultivation of non-transgenic, sex-sorted males |
title_full | Genetic engineering of sex chromosomes for batch cultivation of non-transgenic, sex-sorted males |
title_fullStr | Genetic engineering of sex chromosomes for batch cultivation of non-transgenic, sex-sorted males |
title_full_unstemmed | Genetic engineering of sex chromosomes for batch cultivation of non-transgenic, sex-sorted males |
title_short | Genetic engineering of sex chromosomes for batch cultivation of non-transgenic, sex-sorted males |
title_sort | genetic engineering of sex chromosomes for batch cultivation of non-transgenic, sex-sorted males |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7660900/ https://www.ncbi.nlm.nih.gov/pubmed/33137115 http://dx.doi.org/10.1371/journal.pgen.1009180 |
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