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Transcriptional response of Fusarium oxysporum and Neocosmospora solani challenged with amphotericin B or posaconazole
Some species of fusaria are well-known pathogens of humans, animals and plants. Fusarium oxysporum and Neocosmospora solani (formerly Fusarium solani) cause human infections that range from onychomycosis or keratitis to severe disseminated infections. In general, these infections are difficult to tr...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Microbiology Society
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7660915/ https://www.ncbi.nlm.nih.gov/pubmed/32644917 http://dx.doi.org/10.1099/mic.0.000927 |
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author | Castillo-Castañeda, A. Cañas-Duarte, S. J. Guevara-Suarez, M. Guarro, J. Restrepo, S. Celis Ramírez, A. M. |
author_facet | Castillo-Castañeda, A. Cañas-Duarte, S. J. Guevara-Suarez, M. Guarro, J. Restrepo, S. Celis Ramírez, A. M. |
author_sort | Castillo-Castañeda, A. |
collection | PubMed |
description | Some species of fusaria are well-known pathogens of humans, animals and plants. Fusarium oxysporum and Neocosmospora solani (formerly Fusarium solani) cause human infections that range from onychomycosis or keratitis to severe disseminated infections. In general, these infections are difficult to treat due to poor therapeutic responses in immunocompromised patients. Despite that, little is known about the molecular mechanisms and transcriptional changes responsible for the antifungal resistance in fusaria. To shed light on the transcriptional response to antifungals, we carried out the first reported high-throughput RNA-seq analysis for F. oxysporum and N. solani that had been exposed to amphotericin B (AMB) and posaconazole (PSC). We detected significant differences between the transcriptional profiles of the two species and we found that some oxidation-reduction, metabolic, cellular and transport processes were regulated differentially by both fungi. The same was found with several genes from the ergosterol synthesis, efflux pumps, oxidative stress response and membrane biosynthesis pathways. A significant up-regulation of the C-22 sterol desaturase (ERG5), the sterol 24-C-methyltransferase (ERG6) gene, the glutathione S-transferase (GST) gene and of several members of the major facilitator superfamily (MSF) was demonstrated in this study after treating F. oxysporum with AMB. These results offer a good overview of transcriptional changes after exposure to commonly used antifungals, highlights the genes that are related to resistance mechanisms of these fungi, which will be a valuable tool for identifying causes of failure of treatments. |
format | Online Article Text |
id | pubmed-7660915 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Microbiology Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-76609152020-11-16 Transcriptional response of Fusarium oxysporum and Neocosmospora solani challenged with amphotericin B or posaconazole Castillo-Castañeda, A. Cañas-Duarte, S. J. Guevara-Suarez, M. Guarro, J. Restrepo, S. Celis Ramírez, A. M. Microbiology (Reading) Research Article Some species of fusaria are well-known pathogens of humans, animals and plants. Fusarium oxysporum and Neocosmospora solani (formerly Fusarium solani) cause human infections that range from onychomycosis or keratitis to severe disseminated infections. In general, these infections are difficult to treat due to poor therapeutic responses in immunocompromised patients. Despite that, little is known about the molecular mechanisms and transcriptional changes responsible for the antifungal resistance in fusaria. To shed light on the transcriptional response to antifungals, we carried out the first reported high-throughput RNA-seq analysis for F. oxysporum and N. solani that had been exposed to amphotericin B (AMB) and posaconazole (PSC). We detected significant differences between the transcriptional profiles of the two species and we found that some oxidation-reduction, metabolic, cellular and transport processes were regulated differentially by both fungi. The same was found with several genes from the ergosterol synthesis, efflux pumps, oxidative stress response and membrane biosynthesis pathways. A significant up-regulation of the C-22 sterol desaturase (ERG5), the sterol 24-C-methyltransferase (ERG6) gene, the glutathione S-transferase (GST) gene and of several members of the major facilitator superfamily (MSF) was demonstrated in this study after treating F. oxysporum with AMB. These results offer a good overview of transcriptional changes after exposure to commonly used antifungals, highlights the genes that are related to resistance mechanisms of these fungi, which will be a valuable tool for identifying causes of failure of treatments. Microbiology Society 2020-10 2020-07-09 /pmc/articles/PMC7660915/ /pubmed/32644917 http://dx.doi.org/10.1099/mic.0.000927 Text en © 2020 The Authors https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License. |
spellingShingle | Research Article Castillo-Castañeda, A. Cañas-Duarte, S. J. Guevara-Suarez, M. Guarro, J. Restrepo, S. Celis Ramírez, A. M. Transcriptional response of Fusarium oxysporum and Neocosmospora solani challenged with amphotericin B or posaconazole |
title | Transcriptional response of Fusarium oxysporum and Neocosmospora solani challenged with amphotericin B or posaconazole |
title_full | Transcriptional response of Fusarium oxysporum and Neocosmospora solani challenged with amphotericin B or posaconazole |
title_fullStr | Transcriptional response of Fusarium oxysporum and Neocosmospora solani challenged with amphotericin B or posaconazole |
title_full_unstemmed | Transcriptional response of Fusarium oxysporum and Neocosmospora solani challenged with amphotericin B or posaconazole |
title_short | Transcriptional response of Fusarium oxysporum and Neocosmospora solani challenged with amphotericin B or posaconazole |
title_sort | transcriptional response of fusarium oxysporum and neocosmospora solani challenged with amphotericin b or posaconazole |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7660915/ https://www.ncbi.nlm.nih.gov/pubmed/32644917 http://dx.doi.org/10.1099/mic.0.000927 |
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