Cargando…

Transcriptional response of Fusarium oxysporum and Neocosmospora solani challenged with amphotericin B or posaconazole

Some species of fusaria are well-known pathogens of humans, animals and plants. Fusarium oxysporum and Neocosmospora solani (formerly Fusarium solani) cause human infections that range from onychomycosis or keratitis to severe disseminated infections. In general, these infections are difficult to tr...

Descripción completa

Detalles Bibliográficos
Autores principales: Castillo-Castañeda, A., Cañas-Duarte, S. J., Guevara-Suarez, M., Guarro, J., Restrepo, S., Celis Ramírez, A. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7660915/
https://www.ncbi.nlm.nih.gov/pubmed/32644917
http://dx.doi.org/10.1099/mic.0.000927
_version_ 1783609111813816320
author Castillo-Castañeda, A.
Cañas-Duarte, S. J.
Guevara-Suarez, M.
Guarro, J.
Restrepo, S.
Celis Ramírez, A. M.
author_facet Castillo-Castañeda, A.
Cañas-Duarte, S. J.
Guevara-Suarez, M.
Guarro, J.
Restrepo, S.
Celis Ramírez, A. M.
author_sort Castillo-Castañeda, A.
collection PubMed
description Some species of fusaria are well-known pathogens of humans, animals and plants. Fusarium oxysporum and Neocosmospora solani (formerly Fusarium solani) cause human infections that range from onychomycosis or keratitis to severe disseminated infections. In general, these infections are difficult to treat due to poor therapeutic responses in immunocompromised patients. Despite that, little is known about the molecular mechanisms and transcriptional changes responsible for the antifungal resistance in fusaria. To shed light on the transcriptional response to antifungals, we carried out the first reported high-throughput RNA-seq analysis for F. oxysporum and N. solani that had been exposed to amphotericin B (AMB) and posaconazole (PSC). We detected significant differences between the transcriptional profiles of the two species and we found that some oxidation-reduction, metabolic, cellular and transport processes were regulated differentially by both fungi. The same was found with several genes from the ergosterol synthesis, efflux pumps, oxidative stress response and membrane biosynthesis pathways. A significant up-regulation of the C-22 sterol desaturase (ERG5), the sterol 24-C-methyltransferase (ERG6) gene, the glutathione S-transferase (GST) gene and of several members of the major facilitator superfamily (MSF) was demonstrated in this study after treating F. oxysporum with AMB. These results offer a good overview of transcriptional changes after exposure to commonly used antifungals, highlights the genes that are related to resistance mechanisms of these fungi, which will be a valuable tool for identifying causes of failure of treatments.
format Online
Article
Text
id pubmed-7660915
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Microbiology Society
record_format MEDLINE/PubMed
spelling pubmed-76609152020-11-16 Transcriptional response of Fusarium oxysporum and Neocosmospora solani challenged with amphotericin B or posaconazole Castillo-Castañeda, A. Cañas-Duarte, S. J. Guevara-Suarez, M. Guarro, J. Restrepo, S. Celis Ramírez, A. M. Microbiology (Reading) Research Article Some species of fusaria are well-known pathogens of humans, animals and plants. Fusarium oxysporum and Neocosmospora solani (formerly Fusarium solani) cause human infections that range from onychomycosis or keratitis to severe disseminated infections. In general, these infections are difficult to treat due to poor therapeutic responses in immunocompromised patients. Despite that, little is known about the molecular mechanisms and transcriptional changes responsible for the antifungal resistance in fusaria. To shed light on the transcriptional response to antifungals, we carried out the first reported high-throughput RNA-seq analysis for F. oxysporum and N. solani that had been exposed to amphotericin B (AMB) and posaconazole (PSC). We detected significant differences between the transcriptional profiles of the two species and we found that some oxidation-reduction, metabolic, cellular and transport processes were regulated differentially by both fungi. The same was found with several genes from the ergosterol synthesis, efflux pumps, oxidative stress response and membrane biosynthesis pathways. A significant up-regulation of the C-22 sterol desaturase (ERG5), the sterol 24-C-methyltransferase (ERG6) gene, the glutathione S-transferase (GST) gene and of several members of the major facilitator superfamily (MSF) was demonstrated in this study after treating F. oxysporum with AMB. These results offer a good overview of transcriptional changes after exposure to commonly used antifungals, highlights the genes that are related to resistance mechanisms of these fungi, which will be a valuable tool for identifying causes of failure of treatments. Microbiology Society 2020-10 2020-07-09 /pmc/articles/PMC7660915/ /pubmed/32644917 http://dx.doi.org/10.1099/mic.0.000927 Text en © 2020 The Authors https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License.
spellingShingle Research Article
Castillo-Castañeda, A.
Cañas-Duarte, S. J.
Guevara-Suarez, M.
Guarro, J.
Restrepo, S.
Celis Ramírez, A. M.
Transcriptional response of Fusarium oxysporum and Neocosmospora solani challenged with amphotericin B or posaconazole
title Transcriptional response of Fusarium oxysporum and Neocosmospora solani challenged with amphotericin B or posaconazole
title_full Transcriptional response of Fusarium oxysporum and Neocosmospora solani challenged with amphotericin B or posaconazole
title_fullStr Transcriptional response of Fusarium oxysporum and Neocosmospora solani challenged with amphotericin B or posaconazole
title_full_unstemmed Transcriptional response of Fusarium oxysporum and Neocosmospora solani challenged with amphotericin B or posaconazole
title_short Transcriptional response of Fusarium oxysporum and Neocosmospora solani challenged with amphotericin B or posaconazole
title_sort transcriptional response of fusarium oxysporum and neocosmospora solani challenged with amphotericin b or posaconazole
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7660915/
https://www.ncbi.nlm.nih.gov/pubmed/32644917
http://dx.doi.org/10.1099/mic.0.000927
work_keys_str_mv AT castillocastanedaa transcriptionalresponseoffusariumoxysporumandneocosmosporasolanichallengedwithamphotericinborposaconazole
AT canasduartesj transcriptionalresponseoffusariumoxysporumandneocosmosporasolanichallengedwithamphotericinborposaconazole
AT guevarasuarezm transcriptionalresponseoffusariumoxysporumandneocosmosporasolanichallengedwithamphotericinborposaconazole
AT guarroj transcriptionalresponseoffusariumoxysporumandneocosmosporasolanichallengedwithamphotericinborposaconazole
AT restrepos transcriptionalresponseoffusariumoxysporumandneocosmosporasolanichallengedwithamphotericinborposaconazole
AT celisramirezam transcriptionalresponseoffusariumoxysporumandneocosmosporasolanichallengedwithamphotericinborposaconazole