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Pseudomonas aeruginosa is capable of natural transformation in biofilms
Natural transformation is a mechanism that enables competent bacteria to acquire naked, exogenous DNA from the environment. It is a key process that facilitates the dissemination of antibiotic resistance and virulence determinants throughout bacterial populations. Pseudomonas aeruginosa is an opport...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Microbiology Society
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7660920/ https://www.ncbi.nlm.nih.gov/pubmed/32749953 http://dx.doi.org/10.1099/mic.0.000956 |
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author | Nolan, Laura M. Turnbull, Lynne Katrib, Marilyn Osvath, Sarah R. Losa, Davide Lazenby, James J. Whitchurch, Cynthia B. |
author_facet | Nolan, Laura M. Turnbull, Lynne Katrib, Marilyn Osvath, Sarah R. Losa, Davide Lazenby, James J. Whitchurch, Cynthia B. |
author_sort | Nolan, Laura M. |
collection | PubMed |
description | Natural transformation is a mechanism that enables competent bacteria to acquire naked, exogenous DNA from the environment. It is a key process that facilitates the dissemination of antibiotic resistance and virulence determinants throughout bacterial populations. Pseudomonas aeruginosa is an opportunistic Gram-negative pathogen that produces large quantities of extracellular DNA (eDNA) that is required for biofilm formation. P. aeruginosa has a remarkable level of genome plasticity and diversity that suggests a high degree of horizontal gene transfer and recombination but is thought to be incapable of natural transformation. Here we show that P. aeruginosa possesses homologues of all proteins known to be involved in natural transformation in other bacterial species. We found that P. aeruginosa in biofilms is competent for natural transformation of both genomic and plasmid DNA. Furthermore, we demonstrate that type-IV pili (T4P) facilitate but are not absolutely essential for natural transformation in P. aeruginosa . |
format | Online Article Text |
id | pubmed-7660920 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Microbiology Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-76609202020-11-16 Pseudomonas aeruginosa is capable of natural transformation in biofilms Nolan, Laura M. Turnbull, Lynne Katrib, Marilyn Osvath, Sarah R. Losa, Davide Lazenby, James J. Whitchurch, Cynthia B. Microbiology (Reading) Research Article Natural transformation is a mechanism that enables competent bacteria to acquire naked, exogenous DNA from the environment. It is a key process that facilitates the dissemination of antibiotic resistance and virulence determinants throughout bacterial populations. Pseudomonas aeruginosa is an opportunistic Gram-negative pathogen that produces large quantities of extracellular DNA (eDNA) that is required for biofilm formation. P. aeruginosa has a remarkable level of genome plasticity and diversity that suggests a high degree of horizontal gene transfer and recombination but is thought to be incapable of natural transformation. Here we show that P. aeruginosa possesses homologues of all proteins known to be involved in natural transformation in other bacterial species. We found that P. aeruginosa in biofilms is competent for natural transformation of both genomic and plasmid DNA. Furthermore, we demonstrate that type-IV pili (T4P) facilitate but are not absolutely essential for natural transformation in P. aeruginosa . Microbiology Society 2020-10 2020-08-04 /pmc/articles/PMC7660920/ /pubmed/32749953 http://dx.doi.org/10.1099/mic.0.000956 Text en © 2020 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution. |
spellingShingle | Research Article Nolan, Laura M. Turnbull, Lynne Katrib, Marilyn Osvath, Sarah R. Losa, Davide Lazenby, James J. Whitchurch, Cynthia B. Pseudomonas aeruginosa is capable of natural transformation in biofilms |
title |
Pseudomonas aeruginosa is capable of natural transformation in biofilms |
title_full |
Pseudomonas aeruginosa is capable of natural transformation in biofilms |
title_fullStr |
Pseudomonas aeruginosa is capable of natural transformation in biofilms |
title_full_unstemmed |
Pseudomonas aeruginosa is capable of natural transformation in biofilms |
title_short |
Pseudomonas aeruginosa is capable of natural transformation in biofilms |
title_sort | pseudomonas aeruginosa is capable of natural transformation in biofilms |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7660920/ https://www.ncbi.nlm.nih.gov/pubmed/32749953 http://dx.doi.org/10.1099/mic.0.000956 |
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