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Multiple insecticide resistance target sites in adult field strains of An.gambiae (s.l.) from southeastern Senegal
BACKGROUND: High coverage of long-lasting insecticidal nets (LLINs) and indoor residual spraying (IRS) are the cornerstones of vector control strategy in Senegal where insecticide resistance by the target vectors species is a great of concern. This study explores insecticide susceptibility profile a...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7661151/ https://www.ncbi.nlm.nih.gov/pubmed/33176872 http://dx.doi.org/10.1186/s13071-020-04437-z |
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author | Diouf, El hadji Niang, El hadji Amadou Samb, Badara Diagne, Cheikh Tidiane Diouf, Mbaye Konaté, Abdoulaye Dia, Ibrahima Faye, Ousmane Konaté, Lassana |
author_facet | Diouf, El hadji Niang, El hadji Amadou Samb, Badara Diagne, Cheikh Tidiane Diouf, Mbaye Konaté, Abdoulaye Dia, Ibrahima Faye, Ousmane Konaté, Lassana |
author_sort | Diouf, El hadji |
collection | PubMed |
description | BACKGROUND: High coverage of long-lasting insecticidal nets (LLINs) and indoor residual spraying (IRS) are the cornerstones of vector control strategy in Senegal where insecticide resistance by the target vectors species is a great of concern. This study explores insecticide susceptibility profile and target-site mutations mechanisms within the Anopheles gambiae complex in southeastern Senegal. METHODS: Larvae of Anopheles spp. were collected in two sites from southeastern Senegal Kedougou and Wassadou/Badi in October and November 2014, and reared until adult emergence. Wild F(0) adult mosquitoes were morphologically identified to species. Susceptibility of 3–5-day-old An. gambiae (s.l.) samples to 11 insecticides belonging to the four insecticide classes was assessed using the WHO insecticide susceptibility bioassays. Tested samples were identified using molecular techniques and insecticide resistance target-site mutations (kdr, ace-1 and rdl) were determined. RESULTS: A total of 3742 An. gambiae (s.l.) were exposed to insecticides (2439 from Kedougou and 1303 from Wassadou-Badi). Tests with pyrethroid insecticides and DDT showed high level of resistance in both Kedougou and Wassadou/Badi. Resistance to pirimiphos-methyl and malathion was not detected while resistance to bendoicarb and fenitrothion was confirmed in Kedougou. Of the 745 specimens of An. gambiae (s.l.) genotyped, An. gambiae (s.s.) (71.6%) was the predominant species, followed by An. arabiensis (21.7%), An. coluzzii (6.3%) and hybrids (An. gambiae (s.s.)/An. coluzzii; 0.4%). All target site mutations investigated (Vgsc-1014F, Vgsc-1014S, Ace-1 and Rdl) were found at different frequencies in the species of the Anopheles gambiae complex. Vgsc-1014F mutation was more frequent in An. gambiae (s.s.) and An. coluzzii than An. arabiensis. Vgsc-1014S was present in An. gambiae (s.l.) populations in Wassadou but not in Kedougou. Ace-1 and rdl mutations were more frequent in An. gambiae (s.s.) in comparison to An. arabiensis and An. coluzzii. CONCLUSIONS: Resistance to all the four insecticide classes tested was detected in southeastern Senegal as well as all target site mutations investigated were found. Data will be used by the national Malaria Control Programme. [Image: see text] |
format | Online Article Text |
id | pubmed-7661151 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-76611512020-11-13 Multiple insecticide resistance target sites in adult field strains of An.gambiae (s.l.) from southeastern Senegal Diouf, El hadji Niang, El hadji Amadou Samb, Badara Diagne, Cheikh Tidiane Diouf, Mbaye Konaté, Abdoulaye Dia, Ibrahima Faye, Ousmane Konaté, Lassana Parasit Vectors Research BACKGROUND: High coverage of long-lasting insecticidal nets (LLINs) and indoor residual spraying (IRS) are the cornerstones of vector control strategy in Senegal where insecticide resistance by the target vectors species is a great of concern. This study explores insecticide susceptibility profile and target-site mutations mechanisms within the Anopheles gambiae complex in southeastern Senegal. METHODS: Larvae of Anopheles spp. were collected in two sites from southeastern Senegal Kedougou and Wassadou/Badi in October and November 2014, and reared until adult emergence. Wild F(0) adult mosquitoes were morphologically identified to species. Susceptibility of 3–5-day-old An. gambiae (s.l.) samples to 11 insecticides belonging to the four insecticide classes was assessed using the WHO insecticide susceptibility bioassays. Tested samples were identified using molecular techniques and insecticide resistance target-site mutations (kdr, ace-1 and rdl) were determined. RESULTS: A total of 3742 An. gambiae (s.l.) were exposed to insecticides (2439 from Kedougou and 1303 from Wassadou-Badi). Tests with pyrethroid insecticides and DDT showed high level of resistance in both Kedougou and Wassadou/Badi. Resistance to pirimiphos-methyl and malathion was not detected while resistance to bendoicarb and fenitrothion was confirmed in Kedougou. Of the 745 specimens of An. gambiae (s.l.) genotyped, An. gambiae (s.s.) (71.6%) was the predominant species, followed by An. arabiensis (21.7%), An. coluzzii (6.3%) and hybrids (An. gambiae (s.s.)/An. coluzzii; 0.4%). All target site mutations investigated (Vgsc-1014F, Vgsc-1014S, Ace-1 and Rdl) were found at different frequencies in the species of the Anopheles gambiae complex. Vgsc-1014F mutation was more frequent in An. gambiae (s.s.) and An. coluzzii than An. arabiensis. Vgsc-1014S was present in An. gambiae (s.l.) populations in Wassadou but not in Kedougou. Ace-1 and rdl mutations were more frequent in An. gambiae (s.s.) in comparison to An. arabiensis and An. coluzzii. CONCLUSIONS: Resistance to all the four insecticide classes tested was detected in southeastern Senegal as well as all target site mutations investigated were found. Data will be used by the national Malaria Control Programme. [Image: see text] BioMed Central 2020-11-11 /pmc/articles/PMC7661151/ /pubmed/33176872 http://dx.doi.org/10.1186/s13071-020-04437-z Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Diouf, El hadji Niang, El hadji Amadou Samb, Badara Diagne, Cheikh Tidiane Diouf, Mbaye Konaté, Abdoulaye Dia, Ibrahima Faye, Ousmane Konaté, Lassana Multiple insecticide resistance target sites in adult field strains of An.gambiae (s.l.) from southeastern Senegal |
title | Multiple insecticide resistance target sites in adult field strains of An.gambiae (s.l.) from southeastern Senegal |
title_full | Multiple insecticide resistance target sites in adult field strains of An.gambiae (s.l.) from southeastern Senegal |
title_fullStr | Multiple insecticide resistance target sites in adult field strains of An.gambiae (s.l.) from southeastern Senegal |
title_full_unstemmed | Multiple insecticide resistance target sites in adult field strains of An.gambiae (s.l.) from southeastern Senegal |
title_short | Multiple insecticide resistance target sites in adult field strains of An.gambiae (s.l.) from southeastern Senegal |
title_sort | multiple insecticide resistance target sites in adult field strains of an.gambiae (s.l.) from southeastern senegal |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7661151/ https://www.ncbi.nlm.nih.gov/pubmed/33176872 http://dx.doi.org/10.1186/s13071-020-04437-z |
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