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Long Non-coding RNAs Responsive to Blast Fungus Infection in Rice

BACKGROUND: Long non-coding RNAs (LncRNAs) have emerged as important regulators in many physiological processes in plant. By high-throughput RNA-sequencing, many pathogen-associated LncRNAs were mapped in various plants, and some of them were proved to be involved in plant defense responses. The ric...

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Autores principales: Wang, Lan-Lan, Jin, Jing-Jing, Li, Li-Hua, Qu, Shao-Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7661613/
https://www.ncbi.nlm.nih.gov/pubmed/33180206
http://dx.doi.org/10.1186/s12284-020-00437-w
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author Wang, Lan-Lan
Jin, Jing-Jing
Li, Li-Hua
Qu, Shao-Hong
author_facet Wang, Lan-Lan
Jin, Jing-Jing
Li, Li-Hua
Qu, Shao-Hong
author_sort Wang, Lan-Lan
collection PubMed
description BACKGROUND: Long non-coding RNAs (LncRNAs) have emerged as important regulators in many physiological processes in plant. By high-throughput RNA-sequencing, many pathogen-associated LncRNAs were mapped in various plants, and some of them were proved to be involved in plant defense responses. The rice blast disease caused by Magnaporthe oryzae (M. oryzae) is one of the most destructive diseases in rice. However, M. oryzae-induced LncRNAs in rice is yet to be studied. FINDINGS: We investigated rice LncRNAs that were associated with the rice blast fungus. Totally 83 LncRNAs were up-regulated after blast fungus infection and 78 were down-regulated. Of them, the natural antisense transcripts (NATs) were the most abundant. The expression of some LncRNAs has similar pattern with their host genes or neighboring genes, suggesting a cis function of them in regulating gene transcription level. The deferentially expressed (DE) LncRNAs and genes co-expression analysis revealed some LncRNAs were associated with genes known to be involved in pathogen resistance, and these genes were enriched in terpenoid biosynthesis and defense response by Gene Ontology (GO) enrichment analysis. Interestingly, one of up-regulated DE-intronic RNA was derived from a jasmonate (JA) biosynthetic gene, lipoxygenase RLL (LOX-RLL). Levels of JAs were significantly increased after blast fungus infection. Given that JA is known to regulate blast resistance in rice, we suggested that LncRNA may be involved in JA-mediated rice resistance to blast fungus. CONCLUSIONS: This study identified blast fungus-responsive LncRNAs in rice, which provides another layer of candidates that regulate rice and blast fungus interactions. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12284-020-00437-w.
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spelling pubmed-76616132020-11-16 Long Non-coding RNAs Responsive to Blast Fungus Infection in Rice Wang, Lan-Lan Jin, Jing-Jing Li, Li-Hua Qu, Shao-Hong Rice (N Y) Short Communication BACKGROUND: Long non-coding RNAs (LncRNAs) have emerged as important regulators in many physiological processes in plant. By high-throughput RNA-sequencing, many pathogen-associated LncRNAs were mapped in various plants, and some of them were proved to be involved in plant defense responses. The rice blast disease caused by Magnaporthe oryzae (M. oryzae) is one of the most destructive diseases in rice. However, M. oryzae-induced LncRNAs in rice is yet to be studied. FINDINGS: We investigated rice LncRNAs that were associated with the rice blast fungus. Totally 83 LncRNAs were up-regulated after blast fungus infection and 78 were down-regulated. Of them, the natural antisense transcripts (NATs) were the most abundant. The expression of some LncRNAs has similar pattern with their host genes or neighboring genes, suggesting a cis function of them in regulating gene transcription level. The deferentially expressed (DE) LncRNAs and genes co-expression analysis revealed some LncRNAs were associated with genes known to be involved in pathogen resistance, and these genes were enriched in terpenoid biosynthesis and defense response by Gene Ontology (GO) enrichment analysis. Interestingly, one of up-regulated DE-intronic RNA was derived from a jasmonate (JA) biosynthetic gene, lipoxygenase RLL (LOX-RLL). Levels of JAs were significantly increased after blast fungus infection. Given that JA is known to regulate blast resistance in rice, we suggested that LncRNA may be involved in JA-mediated rice resistance to blast fungus. CONCLUSIONS: This study identified blast fungus-responsive LncRNAs in rice, which provides another layer of candidates that regulate rice and blast fungus interactions. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12284-020-00437-w. Springer US 2020-11-12 /pmc/articles/PMC7661613/ /pubmed/33180206 http://dx.doi.org/10.1186/s12284-020-00437-w Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Short Communication
Wang, Lan-Lan
Jin, Jing-Jing
Li, Li-Hua
Qu, Shao-Hong
Long Non-coding RNAs Responsive to Blast Fungus Infection in Rice
title Long Non-coding RNAs Responsive to Blast Fungus Infection in Rice
title_full Long Non-coding RNAs Responsive to Blast Fungus Infection in Rice
title_fullStr Long Non-coding RNAs Responsive to Blast Fungus Infection in Rice
title_full_unstemmed Long Non-coding RNAs Responsive to Blast Fungus Infection in Rice
title_short Long Non-coding RNAs Responsive to Blast Fungus Infection in Rice
title_sort long non-coding rnas responsive to blast fungus infection in rice
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7661613/
https://www.ncbi.nlm.nih.gov/pubmed/33180206
http://dx.doi.org/10.1186/s12284-020-00437-w
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