Cargando…
Type 2 Diabetes Alters Vascular Cannabinoid Receptor 1 Expression, Phosphorylation Status, and Vasorelaxation in Rat Aorta
Previous studies have suggested a role of the endocannabinoid system in metabolic diseases, such as diabetes. We investigated the effect of diabetes on cannabinoid receptor type 1 (CB(1)) expression and cannabinoid-induced vasorelaxation in rat aorta rings. Aortas from healthy rats and from rats wit...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7662259/ https://www.ncbi.nlm.nih.gov/pubmed/33114620 http://dx.doi.org/10.3390/molecules25214948 |
_version_ | 1783609358391705600 |
---|---|
author | Sánchez-Pastor, Enrique Trujillo, Xóchitl Ramos-Flores, Christian Ríos-Silva, Mónica Andrade, Felipa Cárdenas, Yolitzy Castro, Elena Urzúa, Zorayda Newton-Sánchez, Oscar Huerta, Miguel |
author_facet | Sánchez-Pastor, Enrique Trujillo, Xóchitl Ramos-Flores, Christian Ríos-Silva, Mónica Andrade, Felipa Cárdenas, Yolitzy Castro, Elena Urzúa, Zorayda Newton-Sánchez, Oscar Huerta, Miguel |
author_sort | Sánchez-Pastor, Enrique |
collection | PubMed |
description | Previous studies have suggested a role of the endocannabinoid system in metabolic diseases, such as diabetes. We investigated the effect of diabetes on cannabinoid receptor type 1 (CB(1)) expression and cannabinoid-induced vasorelaxation in rat aorta rings. Aortas from healthy rats and from rats with experimentally induced diabetes were used to compare the vasorelaxant effect of the cannabinoid agonist arachidonylcyclopropylamide (ACPA) and CB(1) expression and localization. After 4–8 weeks of diabetes induction, CB(1) receptor expression and CB(1) phosphorylation were higher in aortic rings, in association with greater vasorelaxation induced by the CB(1) agonist ACPA compared to healthy rats. The vasorelaxant effect observed in healthy rats is similar throughout the study. Further studies are needed to elucidate the implications of CB(1) receptor overexpression in diabetes and its influence on the progression of the cardiovascular complications of this metabolic disease. |
format | Online Article Text |
id | pubmed-7662259 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76622592020-11-14 Type 2 Diabetes Alters Vascular Cannabinoid Receptor 1 Expression, Phosphorylation Status, and Vasorelaxation in Rat Aorta Sánchez-Pastor, Enrique Trujillo, Xóchitl Ramos-Flores, Christian Ríos-Silva, Mónica Andrade, Felipa Cárdenas, Yolitzy Castro, Elena Urzúa, Zorayda Newton-Sánchez, Oscar Huerta, Miguel Molecules Article Previous studies have suggested a role of the endocannabinoid system in metabolic diseases, such as diabetes. We investigated the effect of diabetes on cannabinoid receptor type 1 (CB(1)) expression and cannabinoid-induced vasorelaxation in rat aorta rings. Aortas from healthy rats and from rats with experimentally induced diabetes were used to compare the vasorelaxant effect of the cannabinoid agonist arachidonylcyclopropylamide (ACPA) and CB(1) expression and localization. After 4–8 weeks of diabetes induction, CB(1) receptor expression and CB(1) phosphorylation were higher in aortic rings, in association with greater vasorelaxation induced by the CB(1) agonist ACPA compared to healthy rats. The vasorelaxant effect observed in healthy rats is similar throughout the study. Further studies are needed to elucidate the implications of CB(1) receptor overexpression in diabetes and its influence on the progression of the cardiovascular complications of this metabolic disease. MDPI 2020-10-26 /pmc/articles/PMC7662259/ /pubmed/33114620 http://dx.doi.org/10.3390/molecules25214948 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sánchez-Pastor, Enrique Trujillo, Xóchitl Ramos-Flores, Christian Ríos-Silva, Mónica Andrade, Felipa Cárdenas, Yolitzy Castro, Elena Urzúa, Zorayda Newton-Sánchez, Oscar Huerta, Miguel Type 2 Diabetes Alters Vascular Cannabinoid Receptor 1 Expression, Phosphorylation Status, and Vasorelaxation in Rat Aorta |
title | Type 2 Diabetes Alters Vascular Cannabinoid Receptor 1 Expression, Phosphorylation Status, and Vasorelaxation in Rat Aorta |
title_full | Type 2 Diabetes Alters Vascular Cannabinoid Receptor 1 Expression, Phosphorylation Status, and Vasorelaxation in Rat Aorta |
title_fullStr | Type 2 Diabetes Alters Vascular Cannabinoid Receptor 1 Expression, Phosphorylation Status, and Vasorelaxation in Rat Aorta |
title_full_unstemmed | Type 2 Diabetes Alters Vascular Cannabinoid Receptor 1 Expression, Phosphorylation Status, and Vasorelaxation in Rat Aorta |
title_short | Type 2 Diabetes Alters Vascular Cannabinoid Receptor 1 Expression, Phosphorylation Status, and Vasorelaxation in Rat Aorta |
title_sort | type 2 diabetes alters vascular cannabinoid receptor 1 expression, phosphorylation status, and vasorelaxation in rat aorta |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7662259/ https://www.ncbi.nlm.nih.gov/pubmed/33114620 http://dx.doi.org/10.3390/molecules25214948 |
work_keys_str_mv | AT sanchezpastorenrique type2diabetesaltersvascularcannabinoidreceptor1expressionphosphorylationstatusandvasorelaxationinrataorta AT trujilloxochitl type2diabetesaltersvascularcannabinoidreceptor1expressionphosphorylationstatusandvasorelaxationinrataorta AT ramosfloreschristian type2diabetesaltersvascularcannabinoidreceptor1expressionphosphorylationstatusandvasorelaxationinrataorta AT riossilvamonica type2diabetesaltersvascularcannabinoidreceptor1expressionphosphorylationstatusandvasorelaxationinrataorta AT andradefelipa type2diabetesaltersvascularcannabinoidreceptor1expressionphosphorylationstatusandvasorelaxationinrataorta AT cardenasyolitzy type2diabetesaltersvascularcannabinoidreceptor1expressionphosphorylationstatusandvasorelaxationinrataorta AT castroelena type2diabetesaltersvascularcannabinoidreceptor1expressionphosphorylationstatusandvasorelaxationinrataorta AT urzuazorayda type2diabetesaltersvascularcannabinoidreceptor1expressionphosphorylationstatusandvasorelaxationinrataorta AT newtonsanchezoscar type2diabetesaltersvascularcannabinoidreceptor1expressionphosphorylationstatusandvasorelaxationinrataorta AT huertamiguel type2diabetesaltersvascularcannabinoidreceptor1expressionphosphorylationstatusandvasorelaxationinrataorta |