Cargando…
Assessment of Polyethylene Glycol-Coated Gold Nanoparticle Toxicity and Inflammation In Vivo Using NF-κB Reporter Mice
Polyethylene glycol (PEG) coating of gold nanoparticles (AuNPs) improves AuNP distribution via blood circulation. The use of PEG-coated AuNPs was shown to result in acute injuries to the liver, kidney, and spleen, but long-term toxicity has not been well studied. In this study, we investigated repor...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7662512/ https://www.ncbi.nlm.nih.gov/pubmed/33142808 http://dx.doi.org/10.3390/ijms21218158 |
_version_ | 1783609414178045952 |
---|---|
author | Chen, Tzu-Yin Chen, Mei-Ru Liu, Shan-Wen Lin, Jin-Yan Yang, Ya-Ting Huang, Hsin-Ying Chen, Jen-Kun Yang, Chung-Shi Lin, Kurt Ming-Chao |
author_facet | Chen, Tzu-Yin Chen, Mei-Ru Liu, Shan-Wen Lin, Jin-Yan Yang, Ya-Ting Huang, Hsin-Ying Chen, Jen-Kun Yang, Chung-Shi Lin, Kurt Ming-Chao |
author_sort | Chen, Tzu-Yin |
collection | PubMed |
description | Polyethylene glycol (PEG) coating of gold nanoparticles (AuNPs) improves AuNP distribution via blood circulation. The use of PEG-coated AuNPs was shown to result in acute injuries to the liver, kidney, and spleen, but long-term toxicity has not been well studied. In this study, we investigated reporter induction for up to 90 days in NF-κB transgenic reporter mice following intravenous injection of PEG-coated AuNPs. The results of different doses (1 and 4 μg AuNPs per gram of body weight), particle sizes (13 nm and 30 nm), and PEG surfaces (methoxyl- or carboxymethyl-PEG 5 kDa) were compared. The data showed up to 7-fold NF-κB reporter induction in mouse liver from 3 h to 7 d post PEG-AuNP injection compared to saline-injected control mice, and gradual reduction to a level similar to control by 90 days. Agglomerates of PEG-AuNPs were detected in liver Kupffer cells, but neither gross pathological abnormality in liver sections nor increased activity of liver enzymes were found at 90 days. Injection of PEG-AuNPs led to an increase in collagen in liver sections and elevated total serum cholesterol, although still within the normal range, suggesting that inflammation resulted in mild fibrosis and affected hepatic function. Administrating PEG-AuNPs inevitably results in nanoparticles entrapped in the liver; thus, further investigation is required to fully assess the long-term impacts by PEG-AuNPs on liver health. |
format | Online Article Text |
id | pubmed-7662512 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76625122020-11-14 Assessment of Polyethylene Glycol-Coated Gold Nanoparticle Toxicity and Inflammation In Vivo Using NF-κB Reporter Mice Chen, Tzu-Yin Chen, Mei-Ru Liu, Shan-Wen Lin, Jin-Yan Yang, Ya-Ting Huang, Hsin-Ying Chen, Jen-Kun Yang, Chung-Shi Lin, Kurt Ming-Chao Int J Mol Sci Article Polyethylene glycol (PEG) coating of gold nanoparticles (AuNPs) improves AuNP distribution via blood circulation. The use of PEG-coated AuNPs was shown to result in acute injuries to the liver, kidney, and spleen, but long-term toxicity has not been well studied. In this study, we investigated reporter induction for up to 90 days in NF-κB transgenic reporter mice following intravenous injection of PEG-coated AuNPs. The results of different doses (1 and 4 μg AuNPs per gram of body weight), particle sizes (13 nm and 30 nm), and PEG surfaces (methoxyl- or carboxymethyl-PEG 5 kDa) were compared. The data showed up to 7-fold NF-κB reporter induction in mouse liver from 3 h to 7 d post PEG-AuNP injection compared to saline-injected control mice, and gradual reduction to a level similar to control by 90 days. Agglomerates of PEG-AuNPs were detected in liver Kupffer cells, but neither gross pathological abnormality in liver sections nor increased activity of liver enzymes were found at 90 days. Injection of PEG-AuNPs led to an increase in collagen in liver sections and elevated total serum cholesterol, although still within the normal range, suggesting that inflammation resulted in mild fibrosis and affected hepatic function. Administrating PEG-AuNPs inevitably results in nanoparticles entrapped in the liver; thus, further investigation is required to fully assess the long-term impacts by PEG-AuNPs on liver health. MDPI 2020-10-31 /pmc/articles/PMC7662512/ /pubmed/33142808 http://dx.doi.org/10.3390/ijms21218158 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chen, Tzu-Yin Chen, Mei-Ru Liu, Shan-Wen Lin, Jin-Yan Yang, Ya-Ting Huang, Hsin-Ying Chen, Jen-Kun Yang, Chung-Shi Lin, Kurt Ming-Chao Assessment of Polyethylene Glycol-Coated Gold Nanoparticle Toxicity and Inflammation In Vivo Using NF-κB Reporter Mice |
title | Assessment of Polyethylene Glycol-Coated Gold Nanoparticle Toxicity and Inflammation In Vivo Using NF-κB Reporter Mice |
title_full | Assessment of Polyethylene Glycol-Coated Gold Nanoparticle Toxicity and Inflammation In Vivo Using NF-κB Reporter Mice |
title_fullStr | Assessment of Polyethylene Glycol-Coated Gold Nanoparticle Toxicity and Inflammation In Vivo Using NF-κB Reporter Mice |
title_full_unstemmed | Assessment of Polyethylene Glycol-Coated Gold Nanoparticle Toxicity and Inflammation In Vivo Using NF-κB Reporter Mice |
title_short | Assessment of Polyethylene Glycol-Coated Gold Nanoparticle Toxicity and Inflammation In Vivo Using NF-κB Reporter Mice |
title_sort | assessment of polyethylene glycol-coated gold nanoparticle toxicity and inflammation in vivo using nf-κb reporter mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7662512/ https://www.ncbi.nlm.nih.gov/pubmed/33142808 http://dx.doi.org/10.3390/ijms21218158 |
work_keys_str_mv | AT chentzuyin assessmentofpolyethyleneglycolcoatedgoldnanoparticletoxicityandinflammationinvivousingnfkbreportermice AT chenmeiru assessmentofpolyethyleneglycolcoatedgoldnanoparticletoxicityandinflammationinvivousingnfkbreportermice AT liushanwen assessmentofpolyethyleneglycolcoatedgoldnanoparticletoxicityandinflammationinvivousingnfkbreportermice AT linjinyan assessmentofpolyethyleneglycolcoatedgoldnanoparticletoxicityandinflammationinvivousingnfkbreportermice AT yangyating assessmentofpolyethyleneglycolcoatedgoldnanoparticletoxicityandinflammationinvivousingnfkbreportermice AT huanghsinying assessmentofpolyethyleneglycolcoatedgoldnanoparticletoxicityandinflammationinvivousingnfkbreportermice AT chenjenkun assessmentofpolyethyleneglycolcoatedgoldnanoparticletoxicityandinflammationinvivousingnfkbreportermice AT yangchungshi assessmentofpolyethyleneglycolcoatedgoldnanoparticletoxicityandinflammationinvivousingnfkbreportermice AT linkurtmingchao assessmentofpolyethyleneglycolcoatedgoldnanoparticletoxicityandinflammationinvivousingnfkbreportermice |