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Assessment of Polyethylene Glycol-Coated Gold Nanoparticle Toxicity and Inflammation In Vivo Using NF-κB Reporter Mice

Polyethylene glycol (PEG) coating of gold nanoparticles (AuNPs) improves AuNP distribution via blood circulation. The use of PEG-coated AuNPs was shown to result in acute injuries to the liver, kidney, and spleen, but long-term toxicity has not been well studied. In this study, we investigated repor...

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Autores principales: Chen, Tzu-Yin, Chen, Mei-Ru, Liu, Shan-Wen, Lin, Jin-Yan, Yang, Ya-Ting, Huang, Hsin-Ying, Chen, Jen-Kun, Yang, Chung-Shi, Lin, Kurt Ming-Chao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7662512/
https://www.ncbi.nlm.nih.gov/pubmed/33142808
http://dx.doi.org/10.3390/ijms21218158
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author Chen, Tzu-Yin
Chen, Mei-Ru
Liu, Shan-Wen
Lin, Jin-Yan
Yang, Ya-Ting
Huang, Hsin-Ying
Chen, Jen-Kun
Yang, Chung-Shi
Lin, Kurt Ming-Chao
author_facet Chen, Tzu-Yin
Chen, Mei-Ru
Liu, Shan-Wen
Lin, Jin-Yan
Yang, Ya-Ting
Huang, Hsin-Ying
Chen, Jen-Kun
Yang, Chung-Shi
Lin, Kurt Ming-Chao
author_sort Chen, Tzu-Yin
collection PubMed
description Polyethylene glycol (PEG) coating of gold nanoparticles (AuNPs) improves AuNP distribution via blood circulation. The use of PEG-coated AuNPs was shown to result in acute injuries to the liver, kidney, and spleen, but long-term toxicity has not been well studied. In this study, we investigated reporter induction for up to 90 days in NF-κB transgenic reporter mice following intravenous injection of PEG-coated AuNPs. The results of different doses (1 and 4 μg AuNPs per gram of body weight), particle sizes (13 nm and 30 nm), and PEG surfaces (methoxyl- or carboxymethyl-PEG 5 kDa) were compared. The data showed up to 7-fold NF-κB reporter induction in mouse liver from 3 h to 7 d post PEG-AuNP injection compared to saline-injected control mice, and gradual reduction to a level similar to control by 90 days. Agglomerates of PEG-AuNPs were detected in liver Kupffer cells, but neither gross pathological abnormality in liver sections nor increased activity of liver enzymes were found at 90 days. Injection of PEG-AuNPs led to an increase in collagen in liver sections and elevated total serum cholesterol, although still within the normal range, suggesting that inflammation resulted in mild fibrosis and affected hepatic function. Administrating PEG-AuNPs inevitably results in nanoparticles entrapped in the liver; thus, further investigation is required to fully assess the long-term impacts by PEG-AuNPs on liver health.
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spelling pubmed-76625122020-11-14 Assessment of Polyethylene Glycol-Coated Gold Nanoparticle Toxicity and Inflammation In Vivo Using NF-κB Reporter Mice Chen, Tzu-Yin Chen, Mei-Ru Liu, Shan-Wen Lin, Jin-Yan Yang, Ya-Ting Huang, Hsin-Ying Chen, Jen-Kun Yang, Chung-Shi Lin, Kurt Ming-Chao Int J Mol Sci Article Polyethylene glycol (PEG) coating of gold nanoparticles (AuNPs) improves AuNP distribution via blood circulation. The use of PEG-coated AuNPs was shown to result in acute injuries to the liver, kidney, and spleen, but long-term toxicity has not been well studied. In this study, we investigated reporter induction for up to 90 days in NF-κB transgenic reporter mice following intravenous injection of PEG-coated AuNPs. The results of different doses (1 and 4 μg AuNPs per gram of body weight), particle sizes (13 nm and 30 nm), and PEG surfaces (methoxyl- or carboxymethyl-PEG 5 kDa) were compared. The data showed up to 7-fold NF-κB reporter induction in mouse liver from 3 h to 7 d post PEG-AuNP injection compared to saline-injected control mice, and gradual reduction to a level similar to control by 90 days. Agglomerates of PEG-AuNPs were detected in liver Kupffer cells, but neither gross pathological abnormality in liver sections nor increased activity of liver enzymes were found at 90 days. Injection of PEG-AuNPs led to an increase in collagen in liver sections and elevated total serum cholesterol, although still within the normal range, suggesting that inflammation resulted in mild fibrosis and affected hepatic function. Administrating PEG-AuNPs inevitably results in nanoparticles entrapped in the liver; thus, further investigation is required to fully assess the long-term impacts by PEG-AuNPs on liver health. MDPI 2020-10-31 /pmc/articles/PMC7662512/ /pubmed/33142808 http://dx.doi.org/10.3390/ijms21218158 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Tzu-Yin
Chen, Mei-Ru
Liu, Shan-Wen
Lin, Jin-Yan
Yang, Ya-Ting
Huang, Hsin-Ying
Chen, Jen-Kun
Yang, Chung-Shi
Lin, Kurt Ming-Chao
Assessment of Polyethylene Glycol-Coated Gold Nanoparticle Toxicity and Inflammation In Vivo Using NF-κB Reporter Mice
title Assessment of Polyethylene Glycol-Coated Gold Nanoparticle Toxicity and Inflammation In Vivo Using NF-κB Reporter Mice
title_full Assessment of Polyethylene Glycol-Coated Gold Nanoparticle Toxicity and Inflammation In Vivo Using NF-κB Reporter Mice
title_fullStr Assessment of Polyethylene Glycol-Coated Gold Nanoparticle Toxicity and Inflammation In Vivo Using NF-κB Reporter Mice
title_full_unstemmed Assessment of Polyethylene Glycol-Coated Gold Nanoparticle Toxicity and Inflammation In Vivo Using NF-κB Reporter Mice
title_short Assessment of Polyethylene Glycol-Coated Gold Nanoparticle Toxicity and Inflammation In Vivo Using NF-κB Reporter Mice
title_sort assessment of polyethylene glycol-coated gold nanoparticle toxicity and inflammation in vivo using nf-κb reporter mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7662512/
https://www.ncbi.nlm.nih.gov/pubmed/33142808
http://dx.doi.org/10.3390/ijms21218158
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