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Light-Induced Oxidase Activity of DNAzyme-Modified Quantum Dots

Here, we report the synthesis of a quantum dot (QD)-DNA covalent conjugate to be used as an H(2)O(2)-free DNAzyme system with oxidase activity. Amino-coupling conjugation was carried out between amino-modified oligonucleotides (CatG4-NH(2)) and carboxylated quantum dots (CdTe@COOH QDs). The obtained...

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Autores principales: Żukowski, Krzysztof, Kosman, Joanna, Juskowiak, Bernard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7662946/
https://www.ncbi.nlm.nih.gov/pubmed/33139657
http://dx.doi.org/10.3390/ijms21218190
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author Żukowski, Krzysztof
Kosman, Joanna
Juskowiak, Bernard
author_facet Żukowski, Krzysztof
Kosman, Joanna
Juskowiak, Bernard
author_sort Żukowski, Krzysztof
collection PubMed
description Here, we report the synthesis of a quantum dot (QD)-DNA covalent conjugate to be used as an H(2)O(2)-free DNAzyme system with oxidase activity. Amino-coupling conjugation was carried out between amino-modified oligonucleotides (CatG4-NH(2)) and carboxylated quantum dots (CdTe@COOH QDs). The obtained products were characterized by spectroscopic methods (UV-Vis, fluorescence, circular dichroizm (CD), and IR) and the transmission electron microscopy (TEM) technique. A QD-DNA system with a low polydispersity and high stability in aqueous solutions was successfully obtained. The catalytic activity of the QD-DNA conjugate was examined with Amplex Red and ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate)) indicators using reactive oxygen species (ROS) generated by visible light irradiation. The synthesized QD-DNAzyme exhibited enhanced catalytic activity compared with the reference system (a mixture of QDs and DNAzyme). This proved the assumption that the covalent attachment of DNAzyme to the surface of QD resulted in a beneficial effect on its catalytic activity. The results proved that the QD-DNAzyme system can be used for generation of the signal by light irradiation. The light-induced oxidase activity of the conjugate was demonstrated, proving that the QD-DNAzyme system can be useful for the development of new cellular bioassays, e.g., for the determination of oxygen radical scavengers.
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spelling pubmed-76629462020-11-14 Light-Induced Oxidase Activity of DNAzyme-Modified Quantum Dots Żukowski, Krzysztof Kosman, Joanna Juskowiak, Bernard Int J Mol Sci Article Here, we report the synthesis of a quantum dot (QD)-DNA covalent conjugate to be used as an H(2)O(2)-free DNAzyme system with oxidase activity. Amino-coupling conjugation was carried out between amino-modified oligonucleotides (CatG4-NH(2)) and carboxylated quantum dots (CdTe@COOH QDs). The obtained products were characterized by spectroscopic methods (UV-Vis, fluorescence, circular dichroizm (CD), and IR) and the transmission electron microscopy (TEM) technique. A QD-DNA system with a low polydispersity and high stability in aqueous solutions was successfully obtained. The catalytic activity of the QD-DNA conjugate was examined with Amplex Red and ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate)) indicators using reactive oxygen species (ROS) generated by visible light irradiation. The synthesized QD-DNAzyme exhibited enhanced catalytic activity compared with the reference system (a mixture of QDs and DNAzyme). This proved the assumption that the covalent attachment of DNAzyme to the surface of QD resulted in a beneficial effect on its catalytic activity. The results proved that the QD-DNAzyme system can be used for generation of the signal by light irradiation. The light-induced oxidase activity of the conjugate was demonstrated, proving that the QD-DNAzyme system can be useful for the development of new cellular bioassays, e.g., for the determination of oxygen radical scavengers. MDPI 2020-11-01 /pmc/articles/PMC7662946/ /pubmed/33139657 http://dx.doi.org/10.3390/ijms21218190 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Żukowski, Krzysztof
Kosman, Joanna
Juskowiak, Bernard
Light-Induced Oxidase Activity of DNAzyme-Modified Quantum Dots
title Light-Induced Oxidase Activity of DNAzyme-Modified Quantum Dots
title_full Light-Induced Oxidase Activity of DNAzyme-Modified Quantum Dots
title_fullStr Light-Induced Oxidase Activity of DNAzyme-Modified Quantum Dots
title_full_unstemmed Light-Induced Oxidase Activity of DNAzyme-Modified Quantum Dots
title_short Light-Induced Oxidase Activity of DNAzyme-Modified Quantum Dots
title_sort light-induced oxidase activity of dnazyme-modified quantum dots
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7662946/
https://www.ncbi.nlm.nih.gov/pubmed/33139657
http://dx.doi.org/10.3390/ijms21218190
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AT juskowiakbernard lightinducedoxidaseactivityofdnazymemodifiedquantumdots