Cargando…

The Grapevine Calmodulin-Like Protein Gene CML21 Is Regulated by Alternative Splicing and Involved in Abiotic Stress Response

Calmodulin-like proteins (CMLs) represent a large family of plant calcium sensor proteins involved in the regulation of plant responses to environmental cues and developmental processes. In the present work, we identified four alternatively spliced mRNA forms of the grapevine CML21 gene that encoded...

Descripción completa

Detalles Bibliográficos
Autores principales: Aleynova, Olga A., Kiselev, Konstantin V., Ogneva, Zlata V., Dubrovina, Alexandra S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7663043/
https://www.ncbi.nlm.nih.gov/pubmed/33114685
http://dx.doi.org/10.3390/ijms21217939
_version_ 1783609535489900544
author Aleynova, Olga A.
Kiselev, Konstantin V.
Ogneva, Zlata V.
Dubrovina, Alexandra S.
author_facet Aleynova, Olga A.
Kiselev, Konstantin V.
Ogneva, Zlata V.
Dubrovina, Alexandra S.
author_sort Aleynova, Olga A.
collection PubMed
description Calmodulin-like proteins (CMLs) represent a large family of plant calcium sensor proteins involved in the regulation of plant responses to environmental cues and developmental processes. In the present work, we identified four alternatively spliced mRNA forms of the grapevine CML21 gene that encoded proteins with distinct N-terminal regions. We studied the transcript abundance of CML21v1, CML21v2, CML21v3, and CML21v4 in wild-growing grapevine Vitis amurensis Rupr. in response to desiccation, heat, cold, high salinity, and high mannitol stress using quantitative real-time RT-PCR. The levels of all four splice variants of VaCML21 were highly induced in response to cold stress. In addition, VaCML21v1 and VaCML21v2 forms were highly modulated by all other abiotic stress treatments. Constitutive expression of VaCML21v2 and VaCML21v4 improved biomass accumulation of V. amurensis callus cell cultures under prolonged low temperature stress. Heterologous expression of the grapevine CML21v2 and VaCML21v4 splice variants in Arabidopsis improved survival rates of the transgenic plants after freezing. The VaCML21v2 overexpression enhanced activation of the cold stress-responsive marker genes AtDREB1A and AtDREB2A, while VaCML21v4 overexpression—AtCOR47, AtRD29A, AtRD29B, and AtKIN1 genes after freezing stress in the transgenic Arabidopsis. The results indicate that the grapevine CML21 gene acts as a positive regulator in the plant response to cold stress. The detected variety of CML21 transcripts and their distinct transcriptional responses suggested that this expansion of mRNA variants could contribute to the diversity of grapevine adaptive reactions.
format Online
Article
Text
id pubmed-7663043
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-76630432020-11-14 The Grapevine Calmodulin-Like Protein Gene CML21 Is Regulated by Alternative Splicing and Involved in Abiotic Stress Response Aleynova, Olga A. Kiselev, Konstantin V. Ogneva, Zlata V. Dubrovina, Alexandra S. Int J Mol Sci Article Calmodulin-like proteins (CMLs) represent a large family of plant calcium sensor proteins involved in the regulation of plant responses to environmental cues and developmental processes. In the present work, we identified four alternatively spliced mRNA forms of the grapevine CML21 gene that encoded proteins with distinct N-terminal regions. We studied the transcript abundance of CML21v1, CML21v2, CML21v3, and CML21v4 in wild-growing grapevine Vitis amurensis Rupr. in response to desiccation, heat, cold, high salinity, and high mannitol stress using quantitative real-time RT-PCR. The levels of all four splice variants of VaCML21 were highly induced in response to cold stress. In addition, VaCML21v1 and VaCML21v2 forms were highly modulated by all other abiotic stress treatments. Constitutive expression of VaCML21v2 and VaCML21v4 improved biomass accumulation of V. amurensis callus cell cultures under prolonged low temperature stress. Heterologous expression of the grapevine CML21v2 and VaCML21v4 splice variants in Arabidopsis improved survival rates of the transgenic plants after freezing. The VaCML21v2 overexpression enhanced activation of the cold stress-responsive marker genes AtDREB1A and AtDREB2A, while VaCML21v4 overexpression—AtCOR47, AtRD29A, AtRD29B, and AtKIN1 genes after freezing stress in the transgenic Arabidopsis. The results indicate that the grapevine CML21 gene acts as a positive regulator in the plant response to cold stress. The detected variety of CML21 transcripts and their distinct transcriptional responses suggested that this expansion of mRNA variants could contribute to the diversity of grapevine adaptive reactions. MDPI 2020-10-26 /pmc/articles/PMC7663043/ /pubmed/33114685 http://dx.doi.org/10.3390/ijms21217939 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Aleynova, Olga A.
Kiselev, Konstantin V.
Ogneva, Zlata V.
Dubrovina, Alexandra S.
The Grapevine Calmodulin-Like Protein Gene CML21 Is Regulated by Alternative Splicing and Involved in Abiotic Stress Response
title The Grapevine Calmodulin-Like Protein Gene CML21 Is Regulated by Alternative Splicing and Involved in Abiotic Stress Response
title_full The Grapevine Calmodulin-Like Protein Gene CML21 Is Regulated by Alternative Splicing and Involved in Abiotic Stress Response
title_fullStr The Grapevine Calmodulin-Like Protein Gene CML21 Is Regulated by Alternative Splicing and Involved in Abiotic Stress Response
title_full_unstemmed The Grapevine Calmodulin-Like Protein Gene CML21 Is Regulated by Alternative Splicing and Involved in Abiotic Stress Response
title_short The Grapevine Calmodulin-Like Protein Gene CML21 Is Regulated by Alternative Splicing and Involved in Abiotic Stress Response
title_sort grapevine calmodulin-like protein gene cml21 is regulated by alternative splicing and involved in abiotic stress response
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7663043/
https://www.ncbi.nlm.nih.gov/pubmed/33114685
http://dx.doi.org/10.3390/ijms21217939
work_keys_str_mv AT aleynovaolgaa thegrapevinecalmodulinlikeproteingenecml21isregulatedbyalternativesplicingandinvolvedinabioticstressresponse
AT kiselevkonstantinv thegrapevinecalmodulinlikeproteingenecml21isregulatedbyalternativesplicingandinvolvedinabioticstressresponse
AT ognevazlatav thegrapevinecalmodulinlikeproteingenecml21isregulatedbyalternativesplicingandinvolvedinabioticstressresponse
AT dubrovinaalexandras thegrapevinecalmodulinlikeproteingenecml21isregulatedbyalternativesplicingandinvolvedinabioticstressresponse
AT aleynovaolgaa grapevinecalmodulinlikeproteingenecml21isregulatedbyalternativesplicingandinvolvedinabioticstressresponse
AT kiselevkonstantinv grapevinecalmodulinlikeproteingenecml21isregulatedbyalternativesplicingandinvolvedinabioticstressresponse
AT ognevazlatav grapevinecalmodulinlikeproteingenecml21isregulatedbyalternativesplicingandinvolvedinabioticstressresponse
AT dubrovinaalexandras grapevinecalmodulinlikeproteingenecml21isregulatedbyalternativesplicingandinvolvedinabioticstressresponse