Cargando…
The Effects of Exogenous Lactate Administration on the IGF1/Akt/mTOR Pathway in Rat Skeletal Muscle
We investigated the effects of oral lactate administration on protein synthesis and degradation factors in rats over 2 h after intake. Seven-week-old male Sprague–Dawley rats were randomly divided into four groups (n = 8/group); their blood plasma levels of lactate, glucose, insulin, and insulin-lik...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7663284/ https://www.ncbi.nlm.nih.gov/pubmed/33113811 http://dx.doi.org/10.3390/ijerph17217805 |
_version_ | 1783609592031215616 |
---|---|
author | Kyun, Sunghwan Yoo, Choongsung Park, Hun-Young Kim, Jisu Lim, Kiwon |
author_facet | Kyun, Sunghwan Yoo, Choongsung Park, Hun-Young Kim, Jisu Lim, Kiwon |
author_sort | Kyun, Sunghwan |
collection | PubMed |
description | We investigated the effects of oral lactate administration on protein synthesis and degradation factors in rats over 2 h after intake. Seven-week-old male Sprague–Dawley rats were randomly divided into four groups (n = 8/group); their blood plasma levels of lactate, glucose, insulin, and insulin-like growth factor 1 (IGF1) were examined following sacrifice at 0, 30, 60, or 120 min after sodium lactate (2 g/kg) administration. We measured the mRNA expression levels of protein synthesis-related genes (IGF receptor, protein kinase B (Akt), mammalian target of rapamycin (mTOR)) or degradation-related genes (muscle RING-finger protein-1 (MuRF1), atrogin-1) and analyzed the protein expression and phosphorylation (activation) of Akt and mTOR. Post-administration, the plasma lactate concentration increased to 3.2 mmol/L after 60 min. Plasma glucose remained unchanged throughout, while insulin and IGF1 levels decreased after 30 min. The mRNA levels of IGF receptor and mTOR peaked after 60 min, and Akt expression was significantly upregulated from 30 to 120 min. However, MuRF1 and atrogin-1 expression levels were unaffected. Akt protein phosphorylation did not change significantly, whereas mTOR phosphorylation significantly increased after 30 min. Thus, lactate administration increased the mRNA and protein expression of protein-synthesis factors, suggesting that it can potentially promote skeletal muscle synthesis. |
format | Online Article Text |
id | pubmed-7663284 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76632842020-11-14 The Effects of Exogenous Lactate Administration on the IGF1/Akt/mTOR Pathway in Rat Skeletal Muscle Kyun, Sunghwan Yoo, Choongsung Park, Hun-Young Kim, Jisu Lim, Kiwon Int J Environ Res Public Health Article We investigated the effects of oral lactate administration on protein synthesis and degradation factors in rats over 2 h after intake. Seven-week-old male Sprague–Dawley rats were randomly divided into four groups (n = 8/group); their blood plasma levels of lactate, glucose, insulin, and insulin-like growth factor 1 (IGF1) were examined following sacrifice at 0, 30, 60, or 120 min after sodium lactate (2 g/kg) administration. We measured the mRNA expression levels of protein synthesis-related genes (IGF receptor, protein kinase B (Akt), mammalian target of rapamycin (mTOR)) or degradation-related genes (muscle RING-finger protein-1 (MuRF1), atrogin-1) and analyzed the protein expression and phosphorylation (activation) of Akt and mTOR. Post-administration, the plasma lactate concentration increased to 3.2 mmol/L after 60 min. Plasma glucose remained unchanged throughout, while insulin and IGF1 levels decreased after 30 min. The mRNA levels of IGF receptor and mTOR peaked after 60 min, and Akt expression was significantly upregulated from 30 to 120 min. However, MuRF1 and atrogin-1 expression levels were unaffected. Akt protein phosphorylation did not change significantly, whereas mTOR phosphorylation significantly increased after 30 min. Thus, lactate administration increased the mRNA and protein expression of protein-synthesis factors, suggesting that it can potentially promote skeletal muscle synthesis. MDPI 2020-10-25 2020-11 /pmc/articles/PMC7663284/ /pubmed/33113811 http://dx.doi.org/10.3390/ijerph17217805 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kyun, Sunghwan Yoo, Choongsung Park, Hun-Young Kim, Jisu Lim, Kiwon The Effects of Exogenous Lactate Administration on the IGF1/Akt/mTOR Pathway in Rat Skeletal Muscle |
title | The Effects of Exogenous Lactate Administration on the IGF1/Akt/mTOR Pathway in Rat Skeletal Muscle |
title_full | The Effects of Exogenous Lactate Administration on the IGF1/Akt/mTOR Pathway in Rat Skeletal Muscle |
title_fullStr | The Effects of Exogenous Lactate Administration on the IGF1/Akt/mTOR Pathway in Rat Skeletal Muscle |
title_full_unstemmed | The Effects of Exogenous Lactate Administration on the IGF1/Akt/mTOR Pathway in Rat Skeletal Muscle |
title_short | The Effects of Exogenous Lactate Administration on the IGF1/Akt/mTOR Pathway in Rat Skeletal Muscle |
title_sort | effects of exogenous lactate administration on the igf1/akt/mtor pathway in rat skeletal muscle |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7663284/ https://www.ncbi.nlm.nih.gov/pubmed/33113811 http://dx.doi.org/10.3390/ijerph17217805 |
work_keys_str_mv | AT kyunsunghwan theeffectsofexogenouslactateadministrationontheigf1aktmtorpathwayinratskeletalmuscle AT yoochoongsung theeffectsofexogenouslactateadministrationontheigf1aktmtorpathwayinratskeletalmuscle AT parkhunyoung theeffectsofexogenouslactateadministrationontheigf1aktmtorpathwayinratskeletalmuscle AT kimjisu theeffectsofexogenouslactateadministrationontheigf1aktmtorpathwayinratskeletalmuscle AT limkiwon theeffectsofexogenouslactateadministrationontheigf1aktmtorpathwayinratskeletalmuscle AT kyunsunghwan effectsofexogenouslactateadministrationontheigf1aktmtorpathwayinratskeletalmuscle AT yoochoongsung effectsofexogenouslactateadministrationontheigf1aktmtorpathwayinratskeletalmuscle AT parkhunyoung effectsofexogenouslactateadministrationontheigf1aktmtorpathwayinratskeletalmuscle AT kimjisu effectsofexogenouslactateadministrationontheigf1aktmtorpathwayinratskeletalmuscle AT limkiwon effectsofexogenouslactateadministrationontheigf1aktmtorpathwayinratskeletalmuscle |