Evaluation of Anti-Mycobacterial Compounds in a Silkworm Infection Model with Mycobacteroides abscessus

Among four mycobacteria, Mycobacterium avium, M. intracellulare, M. bovis BCG and Mycobacteroides (My.) abscessus, we established a silkworm infection assay with My. abscessus. When silkworms (fifth-instar larvae, n = 5) were infected through the hemolymph with My. abscessus (7.5 × 10(7) CFU/larva)...

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Autores principales: Hosoda, Kanji, Koyama, Nobuhiro, Hamamoto, Hiroshi, Yagi, Akiho, Uchida, Ryuji, Kanamoto, Akihiko, Tomoda, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7663337/
https://www.ncbi.nlm.nih.gov/pubmed/33121091
http://dx.doi.org/10.3390/molecules25214971
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author Hosoda, Kanji
Koyama, Nobuhiro
Hamamoto, Hiroshi
Yagi, Akiho
Uchida, Ryuji
Kanamoto, Akihiko
Tomoda, Hiroshi
author_facet Hosoda, Kanji
Koyama, Nobuhiro
Hamamoto, Hiroshi
Yagi, Akiho
Uchida, Ryuji
Kanamoto, Akihiko
Tomoda, Hiroshi
author_sort Hosoda, Kanji
collection PubMed
description Among four mycobacteria, Mycobacterium avium, M. intracellulare, M. bovis BCG and Mycobacteroides (My.) abscessus, we established a silkworm infection assay with My. abscessus. When silkworms (fifth-instar larvae, n = 5) were infected through the hemolymph with My. abscessus (7.5 × 10(7) CFU/larva) and bred at 37 °C, they all died around 40 h after injection. Under the conditions, clarithromycin and amikacin, clinically used antimicrobial agents, exhibited therapeutic effects in a dose-dependent manner. Furthermore, five kinds of microbial compounds, lariatin A, nosiheptide, ohmyungsamycins A and B, quinomycin and steffimycin, screened in an in vitro assay to observe anti-My. abscessus activity from 400 microbial products were evaluated in this silkworm infection assay. Lariatin A and nosiheptide exhibited therapeutic efficacy. The silkworm infection model with My. abscessus is useful to screen for therapeutically effective anti-My. abscessus antibiotics.
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spelling pubmed-76633372020-11-14 Evaluation of Anti-Mycobacterial Compounds in a Silkworm Infection Model with Mycobacteroides abscessus Hosoda, Kanji Koyama, Nobuhiro Hamamoto, Hiroshi Yagi, Akiho Uchida, Ryuji Kanamoto, Akihiko Tomoda, Hiroshi Molecules Article Among four mycobacteria, Mycobacterium avium, M. intracellulare, M. bovis BCG and Mycobacteroides (My.) abscessus, we established a silkworm infection assay with My. abscessus. When silkworms (fifth-instar larvae, n = 5) were infected through the hemolymph with My. abscessus (7.5 × 10(7) CFU/larva) and bred at 37 °C, they all died around 40 h after injection. Under the conditions, clarithromycin and amikacin, clinically used antimicrobial agents, exhibited therapeutic effects in a dose-dependent manner. Furthermore, five kinds of microbial compounds, lariatin A, nosiheptide, ohmyungsamycins A and B, quinomycin and steffimycin, screened in an in vitro assay to observe anti-My. abscessus activity from 400 microbial products were evaluated in this silkworm infection assay. Lariatin A and nosiheptide exhibited therapeutic efficacy. The silkworm infection model with My. abscessus is useful to screen for therapeutically effective anti-My. abscessus antibiotics. MDPI 2020-10-27 /pmc/articles/PMC7663337/ /pubmed/33121091 http://dx.doi.org/10.3390/molecules25214971 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hosoda, Kanji
Koyama, Nobuhiro
Hamamoto, Hiroshi
Yagi, Akiho
Uchida, Ryuji
Kanamoto, Akihiko
Tomoda, Hiroshi
Evaluation of Anti-Mycobacterial Compounds in a Silkworm Infection Model with Mycobacteroides abscessus
title Evaluation of Anti-Mycobacterial Compounds in a Silkworm Infection Model with Mycobacteroides abscessus
title_full Evaluation of Anti-Mycobacterial Compounds in a Silkworm Infection Model with Mycobacteroides abscessus
title_fullStr Evaluation of Anti-Mycobacterial Compounds in a Silkworm Infection Model with Mycobacteroides abscessus
title_full_unstemmed Evaluation of Anti-Mycobacterial Compounds in a Silkworm Infection Model with Mycobacteroides abscessus
title_short Evaluation of Anti-Mycobacterial Compounds in a Silkworm Infection Model with Mycobacteroides abscessus
title_sort evaluation of anti-mycobacterial compounds in a silkworm infection model with mycobacteroides abscessus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7663337/
https://www.ncbi.nlm.nih.gov/pubmed/33121091
http://dx.doi.org/10.3390/molecules25214971
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