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Evaluation of Sterilization/Disinfection Methods of Fibrous Polyurethane Scaffolds Designed for Tissue Engineering Applications

Sterilization of a material carries the risk of unwanted changes in physical and chemical structure. The choice of method is a challenge—the process must be efficient, without significantly changing the properties of the material. In the presented studies, we analyzed the effect of selected steriliz...

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Detalles Bibliográficos
Autores principales: Łopianiak, Iwona, Butruk-Raszeja, Beata A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7663349/
https://www.ncbi.nlm.nih.gov/pubmed/33142959
http://dx.doi.org/10.3390/ijms21218092
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author Łopianiak, Iwona
Butruk-Raszeja, Beata A.
author_facet Łopianiak, Iwona
Butruk-Raszeja, Beata A.
author_sort Łopianiak, Iwona
collection PubMed
description Sterilization of a material carries the risk of unwanted changes in physical and chemical structure. The choice of method is a challenge—the process must be efficient, without significantly changing the properties of the material. In the presented studies, we analyzed the effect of selected sterilization/disinfection techniques on the properties of nanofibrous polyurethane biomaterial. Both radiation techniques (UV, gamma, e-beam) and 20 minutes’ contact with 70% EtOH were shown not to achieve 100% sterilization efficiency. The agar diffusion test showed higher sterilization efficiency when using an antimicrobial solution (AMS). At the same time, none of the analyzed techniques significantly altered the morphology and distribution of fiber diameters. EtOH and e-beam sterilization resulted in a significant reduction in material porosity together with an increase in the Young’s modulus. Similarly, AMS sterilization increased the value of Young’s modulus. In most cases, the viability of cells cultured in contact with the sterilized materials was not affected by the sterilization process. Only for UV sterilization, cell viability was significantly lower and reached about 70% of control after 72 h of culture.
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spelling pubmed-76633492020-11-14 Evaluation of Sterilization/Disinfection Methods of Fibrous Polyurethane Scaffolds Designed for Tissue Engineering Applications Łopianiak, Iwona Butruk-Raszeja, Beata A. Int J Mol Sci Article Sterilization of a material carries the risk of unwanted changes in physical and chemical structure. The choice of method is a challenge—the process must be efficient, without significantly changing the properties of the material. In the presented studies, we analyzed the effect of selected sterilization/disinfection techniques on the properties of nanofibrous polyurethane biomaterial. Both radiation techniques (UV, gamma, e-beam) and 20 minutes’ contact with 70% EtOH were shown not to achieve 100% sterilization efficiency. The agar diffusion test showed higher sterilization efficiency when using an antimicrobial solution (AMS). At the same time, none of the analyzed techniques significantly altered the morphology and distribution of fiber diameters. EtOH and e-beam sterilization resulted in a significant reduction in material porosity together with an increase in the Young’s modulus. Similarly, AMS sterilization increased the value of Young’s modulus. In most cases, the viability of cells cultured in contact with the sterilized materials was not affected by the sterilization process. Only for UV sterilization, cell viability was significantly lower and reached about 70% of control after 72 h of culture. MDPI 2020-10-30 /pmc/articles/PMC7663349/ /pubmed/33142959 http://dx.doi.org/10.3390/ijms21218092 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Łopianiak, Iwona
Butruk-Raszeja, Beata A.
Evaluation of Sterilization/Disinfection Methods of Fibrous Polyurethane Scaffolds Designed for Tissue Engineering Applications
title Evaluation of Sterilization/Disinfection Methods of Fibrous Polyurethane Scaffolds Designed for Tissue Engineering Applications
title_full Evaluation of Sterilization/Disinfection Methods of Fibrous Polyurethane Scaffolds Designed for Tissue Engineering Applications
title_fullStr Evaluation of Sterilization/Disinfection Methods of Fibrous Polyurethane Scaffolds Designed for Tissue Engineering Applications
title_full_unstemmed Evaluation of Sterilization/Disinfection Methods of Fibrous Polyurethane Scaffolds Designed for Tissue Engineering Applications
title_short Evaluation of Sterilization/Disinfection Methods of Fibrous Polyurethane Scaffolds Designed for Tissue Engineering Applications
title_sort evaluation of sterilization/disinfection methods of fibrous polyurethane scaffolds designed for tissue engineering applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7663349/
https://www.ncbi.nlm.nih.gov/pubmed/33142959
http://dx.doi.org/10.3390/ijms21218092
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