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A New UPLC-qTOF Approach for Elucidating Furan and 2-Methylfuran Metabolites in Human Urine Samples after Coffee Consumption

We have investigated urine samples after coffee consumption using targeted and untargeted approaches to identify furan and 2-methylfuran metabolites in urine samples by UPLC-qToF. The aim was to establish a fast, robust, and time-saving method involving ultra-performance liquid chromatography-quanti...

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Autores principales: Stegmüller, Simone, Beißmann, Nadine, Kremer, Jonathan Isaak, Mehl, Denise, Baumann, Christian, Richling, Elke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7663408/
https://www.ncbi.nlm.nih.gov/pubmed/33153167
http://dx.doi.org/10.3390/molecules25215104
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author Stegmüller, Simone
Beißmann, Nadine
Kremer, Jonathan Isaak
Mehl, Denise
Baumann, Christian
Richling, Elke
author_facet Stegmüller, Simone
Beißmann, Nadine
Kremer, Jonathan Isaak
Mehl, Denise
Baumann, Christian
Richling, Elke
author_sort Stegmüller, Simone
collection PubMed
description We have investigated urine samples after coffee consumption using targeted and untargeted approaches to identify furan and 2-methylfuran metabolites in urine samples by UPLC-qToF. The aim was to establish a fast, robust, and time-saving method involving ultra-performance liquid chromatography-quantitative time-of-flight tandem mass spectrometry (UPLC-qToF-MS/MS). The developed method detected previously reported metabolites, such as Lys-BDA, and others that had not been previously identified, or only detected in animal or in vitro studies. The developed UPLC-qToF method detected previously reported metabolites, such as lysine-cis-2-butene-1,4-dial (Lys-BDA) adducts, and others that had not been previously identified, or only detected in animal and in vitro studies. In sum, the UPLC-qToF approach provides additional information that may be valuable in future human or animal intervention studies.
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spelling pubmed-76634082020-11-14 A New UPLC-qTOF Approach for Elucidating Furan and 2-Methylfuran Metabolites in Human Urine Samples after Coffee Consumption Stegmüller, Simone Beißmann, Nadine Kremer, Jonathan Isaak Mehl, Denise Baumann, Christian Richling, Elke Molecules Article We have investigated urine samples after coffee consumption using targeted and untargeted approaches to identify furan and 2-methylfuran metabolites in urine samples by UPLC-qToF. The aim was to establish a fast, robust, and time-saving method involving ultra-performance liquid chromatography-quantitative time-of-flight tandem mass spectrometry (UPLC-qToF-MS/MS). The developed method detected previously reported metabolites, such as Lys-BDA, and others that had not been previously identified, or only detected in animal or in vitro studies. The developed UPLC-qToF method detected previously reported metabolites, such as lysine-cis-2-butene-1,4-dial (Lys-BDA) adducts, and others that had not been previously identified, or only detected in animal and in vitro studies. In sum, the UPLC-qToF approach provides additional information that may be valuable in future human or animal intervention studies. MDPI 2020-11-03 /pmc/articles/PMC7663408/ /pubmed/33153167 http://dx.doi.org/10.3390/molecules25215104 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Stegmüller, Simone
Beißmann, Nadine
Kremer, Jonathan Isaak
Mehl, Denise
Baumann, Christian
Richling, Elke
A New UPLC-qTOF Approach for Elucidating Furan and 2-Methylfuran Metabolites in Human Urine Samples after Coffee Consumption
title A New UPLC-qTOF Approach for Elucidating Furan and 2-Methylfuran Metabolites in Human Urine Samples after Coffee Consumption
title_full A New UPLC-qTOF Approach for Elucidating Furan and 2-Methylfuran Metabolites in Human Urine Samples after Coffee Consumption
title_fullStr A New UPLC-qTOF Approach for Elucidating Furan and 2-Methylfuran Metabolites in Human Urine Samples after Coffee Consumption
title_full_unstemmed A New UPLC-qTOF Approach for Elucidating Furan and 2-Methylfuran Metabolites in Human Urine Samples after Coffee Consumption
title_short A New UPLC-qTOF Approach for Elucidating Furan and 2-Methylfuran Metabolites in Human Urine Samples after Coffee Consumption
title_sort new uplc-qtof approach for elucidating furan and 2-methylfuran metabolites in human urine samples after coffee consumption
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7663408/
https://www.ncbi.nlm.nih.gov/pubmed/33153167
http://dx.doi.org/10.3390/molecules25215104
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