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An Immunological Approach to the Biocompatibility of Mesoporous SiO(2)-CaO Nanospheres

Mesoporous bioactive glass nanospheres (NanoMBGs) have high potential for clinical applications. However, the impact of these nanoparticles on the immune system needs to be addressed. In this study, the biocompatibility of SiO(2)-CaO NanoMBGs was evaluated on different mouse immune cells, including...

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Autores principales: Montes-Casado, María, Sanvicente, Adrian, Casarrubios, Laura, Feito, María José, Rojo, José M., Vallet-Regí, María, Arcos, Daniel, Portolés, Pilar, Portolés, María Teresa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7663838/
https://www.ncbi.nlm.nih.gov/pubmed/33167415
http://dx.doi.org/10.3390/ijms21218291
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author Montes-Casado, María
Sanvicente, Adrian
Casarrubios, Laura
Feito, María José
Rojo, José M.
Vallet-Regí, María
Arcos, Daniel
Portolés, Pilar
Portolés, María Teresa
author_facet Montes-Casado, María
Sanvicente, Adrian
Casarrubios, Laura
Feito, María José
Rojo, José M.
Vallet-Regí, María
Arcos, Daniel
Portolés, Pilar
Portolés, María Teresa
author_sort Montes-Casado, María
collection PubMed
description Mesoporous bioactive glass nanospheres (NanoMBGs) have high potential for clinical applications. However, the impact of these nanoparticles on the immune system needs to be addressed. In this study, the biocompatibility of SiO(2)-CaO NanoMBGs was evaluated on different mouse immune cells, including spleen cells subsets, bone marrow-derived dendritic cells (BMDCs), or cell lines like SR.D10 Th2 CD4(+) lymphocytes and DC2.4 dendritic cells. Flow cytometry and confocal microscopy show that the nanoparticles were rapidly and efficiently taken up in vitro by T and B lymphocytes or by specialized antigen-presenting cells (APCs) like dendritic cells (DCs). Nanoparticles were not cytotoxic and had no effect on cell viability or proliferation under T-cell (anti-CD3) or B cell (LPS) stimuli. Besides, NanoMBGs did not affect the balance of spleen cell subsets, or the production of intracellular or secreted pro- and anti-inflammatory cytokines (TNF-α, IFN-γ, IL-2, IL-6, IL-10) by activated T, B, and dendritic cells (DC), as determined by flow cytometry and ELISA. T cell activation surface markers (CD25, CD69 and Induced Costimulator, ICOS) were not altered by NanoMBGs. Maturation of BMDCs or DC2.4 cells in vitro was not altered by NanoMBGs, as shown by expression of Major Histocompatibility Complex (MHC) and costimulatory molecules (CD40, CD80, CD86), or IL-6 secretion. The effect of wortmannin and chlorpromazine indicate a role for phosphoinositide 3-kinase (PI3K), actin and clathrin-dependent pathways in NanoMBG internalization. We thus demonstrate that these NanoMBGs are both non-toxic and non-inflammagenic for murine lymphoid cells and myeloid DCs despite their efficient intake by the cells.
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spelling pubmed-76638382020-11-14 An Immunological Approach to the Biocompatibility of Mesoporous SiO(2)-CaO Nanospheres Montes-Casado, María Sanvicente, Adrian Casarrubios, Laura Feito, María José Rojo, José M. Vallet-Regí, María Arcos, Daniel Portolés, Pilar Portolés, María Teresa Int J Mol Sci Article Mesoporous bioactive glass nanospheres (NanoMBGs) have high potential for clinical applications. However, the impact of these nanoparticles on the immune system needs to be addressed. In this study, the biocompatibility of SiO(2)-CaO NanoMBGs was evaluated on different mouse immune cells, including spleen cells subsets, bone marrow-derived dendritic cells (BMDCs), or cell lines like SR.D10 Th2 CD4(+) lymphocytes and DC2.4 dendritic cells. Flow cytometry and confocal microscopy show that the nanoparticles were rapidly and efficiently taken up in vitro by T and B lymphocytes or by specialized antigen-presenting cells (APCs) like dendritic cells (DCs). Nanoparticles were not cytotoxic and had no effect on cell viability or proliferation under T-cell (anti-CD3) or B cell (LPS) stimuli. Besides, NanoMBGs did not affect the balance of spleen cell subsets, or the production of intracellular or secreted pro- and anti-inflammatory cytokines (TNF-α, IFN-γ, IL-2, IL-6, IL-10) by activated T, B, and dendritic cells (DC), as determined by flow cytometry and ELISA. T cell activation surface markers (CD25, CD69 and Induced Costimulator, ICOS) were not altered by NanoMBGs. Maturation of BMDCs or DC2.4 cells in vitro was not altered by NanoMBGs, as shown by expression of Major Histocompatibility Complex (MHC) and costimulatory molecules (CD40, CD80, CD86), or IL-6 secretion. The effect of wortmannin and chlorpromazine indicate a role for phosphoinositide 3-kinase (PI3K), actin and clathrin-dependent pathways in NanoMBG internalization. We thus demonstrate that these NanoMBGs are both non-toxic and non-inflammagenic for murine lymphoid cells and myeloid DCs despite their efficient intake by the cells. MDPI 2020-11-05 /pmc/articles/PMC7663838/ /pubmed/33167415 http://dx.doi.org/10.3390/ijms21218291 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Montes-Casado, María
Sanvicente, Adrian
Casarrubios, Laura
Feito, María José
Rojo, José M.
Vallet-Regí, María
Arcos, Daniel
Portolés, Pilar
Portolés, María Teresa
An Immunological Approach to the Biocompatibility of Mesoporous SiO(2)-CaO Nanospheres
title An Immunological Approach to the Biocompatibility of Mesoporous SiO(2)-CaO Nanospheres
title_full An Immunological Approach to the Biocompatibility of Mesoporous SiO(2)-CaO Nanospheres
title_fullStr An Immunological Approach to the Biocompatibility of Mesoporous SiO(2)-CaO Nanospheres
title_full_unstemmed An Immunological Approach to the Biocompatibility of Mesoporous SiO(2)-CaO Nanospheres
title_short An Immunological Approach to the Biocompatibility of Mesoporous SiO(2)-CaO Nanospheres
title_sort immunological approach to the biocompatibility of mesoporous sio(2)-cao nanospheres
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7663838/
https://www.ncbi.nlm.nih.gov/pubmed/33167415
http://dx.doi.org/10.3390/ijms21218291
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