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Occurrence of Biogenic Amines Producers in the Wastewater of the Dairy Industry

Out of six samples of wastewater produced in the dairy industry, taken in 2017 at various places of dairy operations, 86 bacterial strains showing decarboxylase activity were isolated. From the wastewater samples, the species of genera Staphylococcus, Lactococcus, Enterococcus, Microbacterium, Kocur...

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Autores principales: Jančová, Petra, Pachlová, Vendula, Čechová, Erika, Cedidlová, Karolína, Šerá, Jana, Pištěková, Hana, Buňka, František, Buňková, Leona
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7663843/
https://www.ncbi.nlm.nih.gov/pubmed/33167326
http://dx.doi.org/10.3390/molecules25215143
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author Jančová, Petra
Pachlová, Vendula
Čechová, Erika
Cedidlová, Karolína
Šerá, Jana
Pištěková, Hana
Buňka, František
Buňková, Leona
author_facet Jančová, Petra
Pachlová, Vendula
Čechová, Erika
Cedidlová, Karolína
Šerá, Jana
Pištěková, Hana
Buňka, František
Buňková, Leona
author_sort Jančová, Petra
collection PubMed
description Out of six samples of wastewater produced in the dairy industry, taken in 2017 at various places of dairy operations, 86 bacterial strains showing decarboxylase activity were isolated. From the wastewater samples, the species of genera Staphylococcus, Lactococcus, Enterococcus, Microbacterium, Kocuria, Acinetobacter, Pseudomonas, Aeromonas, Klebsiella and Enterobacter were identified by the MALDI-TOF MS and biochemical methods. The in vitro produced quantity of eight biogenic amines (BAs) was detected by the HPLC/UV–Vis method. All the isolated bacteria were able to produce four to eight BAs. Tyramine, putrescine and cadaverine belonged to the most frequently produced BAs. Of the isolated bacteria, 41% were able to produce BAs in amounts >100 mg L(−1). Therefore, wastewater embodies a potential vector of transmission of decarboxylase positive microorganisms, which should be taken into consideration in hazard analyses within foodstuff safety control. The parameters of this wastewater (contents of nitrites, nitrates, phosphates, and proteins) were also monitored.
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spelling pubmed-76638432020-11-14 Occurrence of Biogenic Amines Producers in the Wastewater of the Dairy Industry Jančová, Petra Pachlová, Vendula Čechová, Erika Cedidlová, Karolína Šerá, Jana Pištěková, Hana Buňka, František Buňková, Leona Molecules Article Out of six samples of wastewater produced in the dairy industry, taken in 2017 at various places of dairy operations, 86 bacterial strains showing decarboxylase activity were isolated. From the wastewater samples, the species of genera Staphylococcus, Lactococcus, Enterococcus, Microbacterium, Kocuria, Acinetobacter, Pseudomonas, Aeromonas, Klebsiella and Enterobacter were identified by the MALDI-TOF MS and biochemical methods. The in vitro produced quantity of eight biogenic amines (BAs) was detected by the HPLC/UV–Vis method. All the isolated bacteria were able to produce four to eight BAs. Tyramine, putrescine and cadaverine belonged to the most frequently produced BAs. Of the isolated bacteria, 41% were able to produce BAs in amounts >100 mg L(−1). Therefore, wastewater embodies a potential vector of transmission of decarboxylase positive microorganisms, which should be taken into consideration in hazard analyses within foodstuff safety control. The parameters of this wastewater (contents of nitrites, nitrates, phosphates, and proteins) were also monitored. MDPI 2020-11-05 /pmc/articles/PMC7663843/ /pubmed/33167326 http://dx.doi.org/10.3390/molecules25215143 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jančová, Petra
Pachlová, Vendula
Čechová, Erika
Cedidlová, Karolína
Šerá, Jana
Pištěková, Hana
Buňka, František
Buňková, Leona
Occurrence of Biogenic Amines Producers in the Wastewater of the Dairy Industry
title Occurrence of Biogenic Amines Producers in the Wastewater of the Dairy Industry
title_full Occurrence of Biogenic Amines Producers in the Wastewater of the Dairy Industry
title_fullStr Occurrence of Biogenic Amines Producers in the Wastewater of the Dairy Industry
title_full_unstemmed Occurrence of Biogenic Amines Producers in the Wastewater of the Dairy Industry
title_short Occurrence of Biogenic Amines Producers in the Wastewater of the Dairy Industry
title_sort occurrence of biogenic amines producers in the wastewater of the dairy industry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7663843/
https://www.ncbi.nlm.nih.gov/pubmed/33167326
http://dx.doi.org/10.3390/molecules25215143
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