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Tamoxifen Delivery System Based on PEGylated Magnetic MCM-41 Silica

Magnetic iron oxide containing MCM-41 silica (MM) with ~300 nm particle size was developed. The MM material before or after template removal was modified with NH(2)- or COOH-groups and then grafted with PEG chains. The anticancer drug tamoxifen was loaded into the organic groups’ modified and PEGyla...

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Autores principales: Popova, Margarita, Koseva, Neli, Trendafilova, Ivalina, Lazarova, Hristina, Mitova, Violeta, Mihály, Judith, Momekova, Denitsa, Momekov, Georgi, Koleva, Iskra Z., Aleksandrov, Hristiyan A., Vayssilov, Georgi N., Szegedi, Ágnes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7663855/
https://www.ncbi.nlm.nih.gov/pubmed/33158297
http://dx.doi.org/10.3390/molecules25215129
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author Popova, Margarita
Koseva, Neli
Trendafilova, Ivalina
Lazarova, Hristina
Mitova, Violeta
Mihály, Judith
Momekova, Denitsa
Momekov, Georgi
Koleva, Iskra Z.
Aleksandrov, Hristiyan A.
Vayssilov, Georgi N.
Szegedi, Ágnes
author_facet Popova, Margarita
Koseva, Neli
Trendafilova, Ivalina
Lazarova, Hristina
Mitova, Violeta
Mihály, Judith
Momekova, Denitsa
Momekov, Georgi
Koleva, Iskra Z.
Aleksandrov, Hristiyan A.
Vayssilov, Georgi N.
Szegedi, Ágnes
author_sort Popova, Margarita
collection PubMed
description Magnetic iron oxide containing MCM-41 silica (MM) with ~300 nm particle size was developed. The MM material before or after template removal was modified with NH(2)- or COOH-groups and then grafted with PEG chains. The anticancer drug tamoxifen was loaded into the organic groups’ modified and PEGylated nanoparticles by an incipient wetness impregnation procedure. The amount of loaded drug and the release properties depend on whether modification of the nanoparticles was performed before or after the template removal step. The parent and drug-loaded samples were characterized by XRD, N(2) physisorption, thermal gravimetric analysis, and ATR FT-IR spectroscopy. ATR FT-IR spectroscopic data and density functional theory (DFT) calculations supported the interaction between the mesoporous silica surface and tamoxifen molecules and pointed out that the drug molecule interacts more strongly with the silicate surface terminated by silanol groups than with the surface modified with carboxyl groups. A sustained tamoxifen release profile was obtained by an in vitro experiment at pH = 7.0 for the PEGylated formulation modified by COOH groups after the template removal. Free drug and formulated tamoxifen samples were further investigated for antiproliferative activity against MCF-7 cells.
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spelling pubmed-76638552020-11-14 Tamoxifen Delivery System Based on PEGylated Magnetic MCM-41 Silica Popova, Margarita Koseva, Neli Trendafilova, Ivalina Lazarova, Hristina Mitova, Violeta Mihály, Judith Momekova, Denitsa Momekov, Georgi Koleva, Iskra Z. Aleksandrov, Hristiyan A. Vayssilov, Georgi N. Szegedi, Ágnes Molecules Article Magnetic iron oxide containing MCM-41 silica (MM) with ~300 nm particle size was developed. The MM material before or after template removal was modified with NH(2)- or COOH-groups and then grafted with PEG chains. The anticancer drug tamoxifen was loaded into the organic groups’ modified and PEGylated nanoparticles by an incipient wetness impregnation procedure. The amount of loaded drug and the release properties depend on whether modification of the nanoparticles was performed before or after the template removal step. The parent and drug-loaded samples were characterized by XRD, N(2) physisorption, thermal gravimetric analysis, and ATR FT-IR spectroscopy. ATR FT-IR spectroscopic data and density functional theory (DFT) calculations supported the interaction between the mesoporous silica surface and tamoxifen molecules and pointed out that the drug molecule interacts more strongly with the silicate surface terminated by silanol groups than with the surface modified with carboxyl groups. A sustained tamoxifen release profile was obtained by an in vitro experiment at pH = 7.0 for the PEGylated formulation modified by COOH groups after the template removal. Free drug and formulated tamoxifen samples were further investigated for antiproliferative activity against MCF-7 cells. MDPI 2020-11-04 /pmc/articles/PMC7663855/ /pubmed/33158297 http://dx.doi.org/10.3390/molecules25215129 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Popova, Margarita
Koseva, Neli
Trendafilova, Ivalina
Lazarova, Hristina
Mitova, Violeta
Mihály, Judith
Momekova, Denitsa
Momekov, Georgi
Koleva, Iskra Z.
Aleksandrov, Hristiyan A.
Vayssilov, Georgi N.
Szegedi, Ágnes
Tamoxifen Delivery System Based on PEGylated Magnetic MCM-41 Silica
title Tamoxifen Delivery System Based on PEGylated Magnetic MCM-41 Silica
title_full Tamoxifen Delivery System Based on PEGylated Magnetic MCM-41 Silica
title_fullStr Tamoxifen Delivery System Based on PEGylated Magnetic MCM-41 Silica
title_full_unstemmed Tamoxifen Delivery System Based on PEGylated Magnetic MCM-41 Silica
title_short Tamoxifen Delivery System Based on PEGylated Magnetic MCM-41 Silica
title_sort tamoxifen delivery system based on pegylated magnetic mcm-41 silica
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7663855/
https://www.ncbi.nlm.nih.gov/pubmed/33158297
http://dx.doi.org/10.3390/molecules25215129
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