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Efficient isolation of protoplasts from rice calli with pause points and its application in transient gene expression and genome editing assays

BACKGROUND: An efficient in vivo transient transfection system using protoplasts is an important tool to study gene expression, metabolic pathways, and multiple mutagenesis parameters in plants. Although rice protoplasts can be isolated from germinated seedlings or cell suspension culture, preparati...

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Detalles Bibliográficos
Autores principales:	Poddar, Snigdha, Tanaka, Jaclyn, Cate, Jamie H. D., Staskawicz, Brian, Cho, Myeong-Je
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2020
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7663885/ https://www.ncbi.nlm.nih.gov/pubmed/33292393 http://dx.doi.org/10.1186/s13007-020-00692-4

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