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An Odorant Binding Protein (SaveOBP9) Involved in Chemoreception of the Wheat Aphid Sitobion avenae
Odorant binding proteins play a key role in the olfactory system and are involved in the odor perception and discrimination of insects. To investigate the potential physiological functions of SaveOBP9 in Sitobion avenae, fluorescence ligand binding experiments, molecular docking, RNA interference, a...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7664216/ https://www.ncbi.nlm.nih.gov/pubmed/33172024 http://dx.doi.org/10.3390/ijms21218331 |
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author | Ullah, Rana Muhammad Kaleem Quershi, Sundas Rana Adeel, Muhammad Muzammal Abdelnabby, Hazem Waris, Muhammad Irfan Duan, Shuang-Gang Wang, Man-Qun |
author_facet | Ullah, Rana Muhammad Kaleem Quershi, Sundas Rana Adeel, Muhammad Muzammal Abdelnabby, Hazem Waris, Muhammad Irfan Duan, Shuang-Gang Wang, Man-Qun |
author_sort | Ullah, Rana Muhammad Kaleem |
collection | PubMed |
description | Odorant binding proteins play a key role in the olfactory system and are involved in the odor perception and discrimination of insects. To investigate the potential physiological functions of SaveOBP9 in Sitobion avenae, fluorescence ligand binding experiments, molecular docking, RNA interference, and behavioral tests were performed. Fluorescence binding assay results showed that SaveOBP9 had broad and high (Ki < 10 μM) binding abilities with most of the wheat volatiles, but was more obvious at pH 7.4 than pH 5.0. The binding sites of SaveOBP9 to the volatiles were predicted well by three-dimensional docking structure modeling and molecular docking. Moreover, S. avenae showed a strong behavioral response with the four compounds of wheat. The reduction in mRNA transcript levels after the RNA interference significantly reduced the expression level of SaveOBP9 and induced the non-significant response of S. avenae to the tetradecane, octanal, decanal, and hexadecane. This study provides evidence that SaveOBP9 might be involved in the chemoreception of wheat volatile organic compounds and can successfully contribute in the integrated management programs of S. avenae. |
format | Online Article Text |
id | pubmed-7664216 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76642162020-11-14 An Odorant Binding Protein (SaveOBP9) Involved in Chemoreception of the Wheat Aphid Sitobion avenae Ullah, Rana Muhammad Kaleem Quershi, Sundas Rana Adeel, Muhammad Muzammal Abdelnabby, Hazem Waris, Muhammad Irfan Duan, Shuang-Gang Wang, Man-Qun Int J Mol Sci Article Odorant binding proteins play a key role in the olfactory system and are involved in the odor perception and discrimination of insects. To investigate the potential physiological functions of SaveOBP9 in Sitobion avenae, fluorescence ligand binding experiments, molecular docking, RNA interference, and behavioral tests were performed. Fluorescence binding assay results showed that SaveOBP9 had broad and high (Ki < 10 μM) binding abilities with most of the wheat volatiles, but was more obvious at pH 7.4 than pH 5.0. The binding sites of SaveOBP9 to the volatiles were predicted well by three-dimensional docking structure modeling and molecular docking. Moreover, S. avenae showed a strong behavioral response with the four compounds of wheat. The reduction in mRNA transcript levels after the RNA interference significantly reduced the expression level of SaveOBP9 and induced the non-significant response of S. avenae to the tetradecane, octanal, decanal, and hexadecane. This study provides evidence that SaveOBP9 might be involved in the chemoreception of wheat volatile organic compounds and can successfully contribute in the integrated management programs of S. avenae. MDPI 2020-11-06 /pmc/articles/PMC7664216/ /pubmed/33172024 http://dx.doi.org/10.3390/ijms21218331 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ullah, Rana Muhammad Kaleem Quershi, Sundas Rana Adeel, Muhammad Muzammal Abdelnabby, Hazem Waris, Muhammad Irfan Duan, Shuang-Gang Wang, Man-Qun An Odorant Binding Protein (SaveOBP9) Involved in Chemoreception of the Wheat Aphid Sitobion avenae |
title | An Odorant Binding Protein (SaveOBP9) Involved in Chemoreception of the Wheat Aphid Sitobion avenae |
title_full | An Odorant Binding Protein (SaveOBP9) Involved in Chemoreception of the Wheat Aphid Sitobion avenae |
title_fullStr | An Odorant Binding Protein (SaveOBP9) Involved in Chemoreception of the Wheat Aphid Sitobion avenae |
title_full_unstemmed | An Odorant Binding Protein (SaveOBP9) Involved in Chemoreception of the Wheat Aphid Sitobion avenae |
title_short | An Odorant Binding Protein (SaveOBP9) Involved in Chemoreception of the Wheat Aphid Sitobion avenae |
title_sort | odorant binding protein (saveobp9) involved in chemoreception of the wheat aphid sitobion avenae |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7664216/ https://www.ncbi.nlm.nih.gov/pubmed/33172024 http://dx.doi.org/10.3390/ijms21218331 |
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