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An Odorant Binding Protein (SaveOBP9) Involved in Chemoreception of the Wheat Aphid Sitobion avenae

Odorant binding proteins play a key role in the olfactory system and are involved in the odor perception and discrimination of insects. To investigate the potential physiological functions of SaveOBP9 in Sitobion avenae, fluorescence ligand binding experiments, molecular docking, RNA interference, a...

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Autores principales: Ullah, Rana Muhammad Kaleem, Quershi, Sundas Rana, Adeel, Muhammad Muzammal, Abdelnabby, Hazem, Waris, Muhammad Irfan, Duan, Shuang-Gang, Wang, Man-Qun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7664216/
https://www.ncbi.nlm.nih.gov/pubmed/33172024
http://dx.doi.org/10.3390/ijms21218331
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author Ullah, Rana Muhammad Kaleem
Quershi, Sundas Rana
Adeel, Muhammad Muzammal
Abdelnabby, Hazem
Waris, Muhammad Irfan
Duan, Shuang-Gang
Wang, Man-Qun
author_facet Ullah, Rana Muhammad Kaleem
Quershi, Sundas Rana
Adeel, Muhammad Muzammal
Abdelnabby, Hazem
Waris, Muhammad Irfan
Duan, Shuang-Gang
Wang, Man-Qun
author_sort Ullah, Rana Muhammad Kaleem
collection PubMed
description Odorant binding proteins play a key role in the olfactory system and are involved in the odor perception and discrimination of insects. To investigate the potential physiological functions of SaveOBP9 in Sitobion avenae, fluorescence ligand binding experiments, molecular docking, RNA interference, and behavioral tests were performed. Fluorescence binding assay results showed that SaveOBP9 had broad and high (Ki < 10 μM) binding abilities with most of the wheat volatiles, but was more obvious at pH 7.4 than pH 5.0. The binding sites of SaveOBP9 to the volatiles were predicted well by three-dimensional docking structure modeling and molecular docking. Moreover, S. avenae showed a strong behavioral response with the four compounds of wheat. The reduction in mRNA transcript levels after the RNA interference significantly reduced the expression level of SaveOBP9 and induced the non-significant response of S. avenae to the tetradecane, octanal, decanal, and hexadecane. This study provides evidence that SaveOBP9 might be involved in the chemoreception of wheat volatile organic compounds and can successfully contribute in the integrated management programs of S. avenae.
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spelling pubmed-76642162020-11-14 An Odorant Binding Protein (SaveOBP9) Involved in Chemoreception of the Wheat Aphid Sitobion avenae Ullah, Rana Muhammad Kaleem Quershi, Sundas Rana Adeel, Muhammad Muzammal Abdelnabby, Hazem Waris, Muhammad Irfan Duan, Shuang-Gang Wang, Man-Qun Int J Mol Sci Article Odorant binding proteins play a key role in the olfactory system and are involved in the odor perception and discrimination of insects. To investigate the potential physiological functions of SaveOBP9 in Sitobion avenae, fluorescence ligand binding experiments, molecular docking, RNA interference, and behavioral tests were performed. Fluorescence binding assay results showed that SaveOBP9 had broad and high (Ki < 10 μM) binding abilities with most of the wheat volatiles, but was more obvious at pH 7.4 than pH 5.0. The binding sites of SaveOBP9 to the volatiles were predicted well by three-dimensional docking structure modeling and molecular docking. Moreover, S. avenae showed a strong behavioral response with the four compounds of wheat. The reduction in mRNA transcript levels after the RNA interference significantly reduced the expression level of SaveOBP9 and induced the non-significant response of S. avenae to the tetradecane, octanal, decanal, and hexadecane. This study provides evidence that SaveOBP9 might be involved in the chemoreception of wheat volatile organic compounds and can successfully contribute in the integrated management programs of S. avenae. MDPI 2020-11-06 /pmc/articles/PMC7664216/ /pubmed/33172024 http://dx.doi.org/10.3390/ijms21218331 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ullah, Rana Muhammad Kaleem
Quershi, Sundas Rana
Adeel, Muhammad Muzammal
Abdelnabby, Hazem
Waris, Muhammad Irfan
Duan, Shuang-Gang
Wang, Man-Qun
An Odorant Binding Protein (SaveOBP9) Involved in Chemoreception of the Wheat Aphid Sitobion avenae
title An Odorant Binding Protein (SaveOBP9) Involved in Chemoreception of the Wheat Aphid Sitobion avenae
title_full An Odorant Binding Protein (SaveOBP9) Involved in Chemoreception of the Wheat Aphid Sitobion avenae
title_fullStr An Odorant Binding Protein (SaveOBP9) Involved in Chemoreception of the Wheat Aphid Sitobion avenae
title_full_unstemmed An Odorant Binding Protein (SaveOBP9) Involved in Chemoreception of the Wheat Aphid Sitobion avenae
title_short An Odorant Binding Protein (SaveOBP9) Involved in Chemoreception of the Wheat Aphid Sitobion avenae
title_sort odorant binding protein (saveobp9) involved in chemoreception of the wheat aphid sitobion avenae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7664216/
https://www.ncbi.nlm.nih.gov/pubmed/33172024
http://dx.doi.org/10.3390/ijms21218331
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