A novel liposome-polymer hybrid nanoparticles delivering a multi-epitope self-replication DNA vaccine and its preliminary immune evaluation in experimental animals

DNA vaccine is an attractive immune platform for the prevention and treatment of infectious diseases, but existing disadvantages limit its use in preclinical and clinical assays, such as weak immunogenicity and short half-life. Here, we reported a novel liposome-polymer hybrid nanoparticles (pSFV-ME...

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Autores principales: Zhao, Zhangting, Ma, Xingyuan, Zhang, Ruihuan, Hu, Fabiao, Zhang, Tong, Liu, Yuping, Han, Myong Hun, You, Fang, Yang, Yi, Zheng, Wenyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier Inc. 2021
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7664365/
https://www.ncbi.nlm.nih.gov/pubmed/33197626
http://dx.doi.org/10.1016/j.nano.2020.102338
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author Zhao, Zhangting
Ma, Xingyuan
Zhang, Ruihuan
Hu, Fabiao
Zhang, Tong
Liu, Yuping
Han, Myong Hun
You, Fang
Yang, Yi
Zheng, Wenyun
author_facet Zhao, Zhangting
Ma, Xingyuan
Zhang, Ruihuan
Hu, Fabiao
Zhang, Tong
Liu, Yuping
Han, Myong Hun
You, Fang
Yang, Yi
Zheng, Wenyun
author_sort Zhao, Zhangting
collection PubMed
description DNA vaccine is an attractive immune platform for the prevention and treatment of infectious diseases, but existing disadvantages limit its use in preclinical and clinical assays, such as weak immunogenicity and short half-life. Here, we reported a novel liposome-polymer hybrid nanoparticles (pSFV-MEG/LNPs) consisting of a biodegradable core (mPEG-PLGA) and a hydrophilic shell (lecithin/PEG-DSPE-Mal 2000) for delivering a multi-epitope self-replication DNA vaccine (pSFV-MEG). The pSFV-MEG/LNPs with optimal particle size (161.61 ± 15.63 nm) and high encapsulation efficiency (87.60 ± 8.73%) induced a strong humoral (3.22-fold) and cellular immune responses (1.60-fold) compared to PBS. Besides, the humoral and cellular immune responses of pSFV-MEG/LNPs were 1.58- and 1.05-fold than that of pSFV-MEG. All results confirmed that LNPs was a very promising tool to enhance the humoral and cellular immune responses of pSFV-MEG. In addition, the rational design and delivery platform can be used for the development of DNA vaccines for other infectious diseases.
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spelling pubmed-76643652020-11-16 A novel liposome-polymer hybrid nanoparticles delivering a multi-epitope self-replication DNA vaccine and its preliminary immune evaluation in experimental animals Zhao, Zhangting Ma, Xingyuan Zhang, Ruihuan Hu, Fabiao Zhang, Tong Liu, Yuping Han, Myong Hun You, Fang Yang, Yi Zheng, Wenyun Nanomedicine Original Article DNA vaccine is an attractive immune platform for the prevention and treatment of infectious diseases, but existing disadvantages limit its use in preclinical and clinical assays, such as weak immunogenicity and short half-life. Here, we reported a novel liposome-polymer hybrid nanoparticles (pSFV-MEG/LNPs) consisting of a biodegradable core (mPEG-PLGA) and a hydrophilic shell (lecithin/PEG-DSPE-Mal 2000) for delivering a multi-epitope self-replication DNA vaccine (pSFV-MEG). The pSFV-MEG/LNPs with optimal particle size (161.61 ± 15.63 nm) and high encapsulation efficiency (87.60 ± 8.73%) induced a strong humoral (3.22-fold) and cellular immune responses (1.60-fold) compared to PBS. Besides, the humoral and cellular immune responses of pSFV-MEG/LNPs were 1.58- and 1.05-fold than that of pSFV-MEG. All results confirmed that LNPs was a very promising tool to enhance the humoral and cellular immune responses of pSFV-MEG. In addition, the rational design and delivery platform can be used for the development of DNA vaccines for other infectious diseases. Published by Elsevier Inc. 2021-07 2020-11-13 /pmc/articles/PMC7664365/ /pubmed/33197626 http://dx.doi.org/10.1016/j.nano.2020.102338 Text en © 2020 Published by Elsevier Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Original Article
Zhao, Zhangting
Ma, Xingyuan
Zhang, Ruihuan
Hu, Fabiao
Zhang, Tong
Liu, Yuping
Han, Myong Hun
You, Fang
Yang, Yi
Zheng, Wenyun
A novel liposome-polymer hybrid nanoparticles delivering a multi-epitope self-replication DNA vaccine and its preliminary immune evaluation in experimental animals
title A novel liposome-polymer hybrid nanoparticles delivering a multi-epitope self-replication DNA vaccine and its preliminary immune evaluation in experimental animals
title_full A novel liposome-polymer hybrid nanoparticles delivering a multi-epitope self-replication DNA vaccine and its preliminary immune evaluation in experimental animals
title_fullStr A novel liposome-polymer hybrid nanoparticles delivering a multi-epitope self-replication DNA vaccine and its preliminary immune evaluation in experimental animals
title_full_unstemmed A novel liposome-polymer hybrid nanoparticles delivering a multi-epitope self-replication DNA vaccine and its preliminary immune evaluation in experimental animals
title_short A novel liposome-polymer hybrid nanoparticles delivering a multi-epitope self-replication DNA vaccine and its preliminary immune evaluation in experimental animals
title_sort novel liposome-polymer hybrid nanoparticles delivering a multi-epitope self-replication dna vaccine and its preliminary immune evaluation in experimental animals
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7664365/
https://www.ncbi.nlm.nih.gov/pubmed/33197626
http://dx.doi.org/10.1016/j.nano.2020.102338
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