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Cadmium Stress Reprograms ROS/RNS Homeostasis in Phytophthora infestans (Mont.) de Bary

Heavy metal pollution causes many soils to become a toxic environment not only for plants, but also microorganisms; however, little is known how heavy metal contaminated environment affects metabolism of phytopathogens and their capability of infecting host plants. In this study the oomycete Phytoph...

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Autores principales: Gajewska, Joanna, Azzahra, Nur Afifah, Bingöl, Özgün Ali, Izbiańska-Jankowska, Karolina, Jelonek, Tomasz, Deckert, Joanna, Floryszak-Wieczorek, Jolanta, Arasimowicz-Jelonek, Magdalena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7664633/
https://www.ncbi.nlm.nih.gov/pubmed/33171629
http://dx.doi.org/10.3390/ijms21218375
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author Gajewska, Joanna
Azzahra, Nur Afifah
Bingöl, Özgün Ali
Izbiańska-Jankowska, Karolina
Jelonek, Tomasz
Deckert, Joanna
Floryszak-Wieczorek, Jolanta
Arasimowicz-Jelonek, Magdalena
author_facet Gajewska, Joanna
Azzahra, Nur Afifah
Bingöl, Özgün Ali
Izbiańska-Jankowska, Karolina
Jelonek, Tomasz
Deckert, Joanna
Floryszak-Wieczorek, Jolanta
Arasimowicz-Jelonek, Magdalena
author_sort Gajewska, Joanna
collection PubMed
description Heavy metal pollution causes many soils to become a toxic environment not only for plants, but also microorganisms; however, little is known how heavy metal contaminated environment affects metabolism of phytopathogens and their capability of infecting host plants. In this study the oomycete Phytophthora infestans (Mont.) de Bary, the most harmful pathogen of potato, growing under moderate cadmium stress (Cd, 5 mg/L) showed nitro-oxidative imbalance associated with an enhanced antioxidant response. Cadmium notably elevated the level of nitric oxide, superoxide and peroxynitrite that stimulated nitrative modifications within the RNA and DNA pools in the phytopathogen structures. In contrast, the protein pool undergoing nitration was diminished confirming that protein tyrosine nitration is a flexible element of the oomycete adaptive strategy to heavy metal stress. Finally, to verify whether Cd is able to modify P. infestans pathogenicity, a disease index and molecular assessment of disease progress were analysed indicating that Cd stress enhanced aggressiveness of vr P. infestans towards various potato cultivars. Taken together, Cd not only affected hyphal growth rate and caused biochemical changes in P. infestans structures, but accelerated the pathogenicity as well. The nitro-oxidative homeostasis imbalance underlies the phytopathogen adaptive strategy and survival in the heavy metal contaminated environment.
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spelling pubmed-76646332020-11-14 Cadmium Stress Reprograms ROS/RNS Homeostasis in Phytophthora infestans (Mont.) de Bary Gajewska, Joanna Azzahra, Nur Afifah Bingöl, Özgün Ali Izbiańska-Jankowska, Karolina Jelonek, Tomasz Deckert, Joanna Floryszak-Wieczorek, Jolanta Arasimowicz-Jelonek, Magdalena Int J Mol Sci Article Heavy metal pollution causes many soils to become a toxic environment not only for plants, but also microorganisms; however, little is known how heavy metal contaminated environment affects metabolism of phytopathogens and their capability of infecting host plants. In this study the oomycete Phytophthora infestans (Mont.) de Bary, the most harmful pathogen of potato, growing under moderate cadmium stress (Cd, 5 mg/L) showed nitro-oxidative imbalance associated with an enhanced antioxidant response. Cadmium notably elevated the level of nitric oxide, superoxide and peroxynitrite that stimulated nitrative modifications within the RNA and DNA pools in the phytopathogen structures. In contrast, the protein pool undergoing nitration was diminished confirming that protein tyrosine nitration is a flexible element of the oomycete adaptive strategy to heavy metal stress. Finally, to verify whether Cd is able to modify P. infestans pathogenicity, a disease index and molecular assessment of disease progress were analysed indicating that Cd stress enhanced aggressiveness of vr P. infestans towards various potato cultivars. Taken together, Cd not only affected hyphal growth rate and caused biochemical changes in P. infestans structures, but accelerated the pathogenicity as well. The nitro-oxidative homeostasis imbalance underlies the phytopathogen adaptive strategy and survival in the heavy metal contaminated environment. MDPI 2020-11-08 /pmc/articles/PMC7664633/ /pubmed/33171629 http://dx.doi.org/10.3390/ijms21218375 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gajewska, Joanna
Azzahra, Nur Afifah
Bingöl, Özgün Ali
Izbiańska-Jankowska, Karolina
Jelonek, Tomasz
Deckert, Joanna
Floryszak-Wieczorek, Jolanta
Arasimowicz-Jelonek, Magdalena
Cadmium Stress Reprograms ROS/RNS Homeostasis in Phytophthora infestans (Mont.) de Bary
title Cadmium Stress Reprograms ROS/RNS Homeostasis in Phytophthora infestans (Mont.) de Bary
title_full Cadmium Stress Reprograms ROS/RNS Homeostasis in Phytophthora infestans (Mont.) de Bary
title_fullStr Cadmium Stress Reprograms ROS/RNS Homeostasis in Phytophthora infestans (Mont.) de Bary
title_full_unstemmed Cadmium Stress Reprograms ROS/RNS Homeostasis in Phytophthora infestans (Mont.) de Bary
title_short Cadmium Stress Reprograms ROS/RNS Homeostasis in Phytophthora infestans (Mont.) de Bary
title_sort cadmium stress reprograms ros/rns homeostasis in phytophthora infestans (mont.) de bary
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7664633/
https://www.ncbi.nlm.nih.gov/pubmed/33171629
http://dx.doi.org/10.3390/ijms21218375
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