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Cadmium Stress Reprograms ROS/RNS Homeostasis in Phytophthora infestans (Mont.) de Bary
Heavy metal pollution causes many soils to become a toxic environment not only for plants, but also microorganisms; however, little is known how heavy metal contaminated environment affects metabolism of phytopathogens and their capability of infecting host plants. In this study the oomycete Phytoph...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7664633/ https://www.ncbi.nlm.nih.gov/pubmed/33171629 http://dx.doi.org/10.3390/ijms21218375 |
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author | Gajewska, Joanna Azzahra, Nur Afifah Bingöl, Özgün Ali Izbiańska-Jankowska, Karolina Jelonek, Tomasz Deckert, Joanna Floryszak-Wieczorek, Jolanta Arasimowicz-Jelonek, Magdalena |
author_facet | Gajewska, Joanna Azzahra, Nur Afifah Bingöl, Özgün Ali Izbiańska-Jankowska, Karolina Jelonek, Tomasz Deckert, Joanna Floryszak-Wieczorek, Jolanta Arasimowicz-Jelonek, Magdalena |
author_sort | Gajewska, Joanna |
collection | PubMed |
description | Heavy metal pollution causes many soils to become a toxic environment not only for plants, but also microorganisms; however, little is known how heavy metal contaminated environment affects metabolism of phytopathogens and their capability of infecting host plants. In this study the oomycete Phytophthora infestans (Mont.) de Bary, the most harmful pathogen of potato, growing under moderate cadmium stress (Cd, 5 mg/L) showed nitro-oxidative imbalance associated with an enhanced antioxidant response. Cadmium notably elevated the level of nitric oxide, superoxide and peroxynitrite that stimulated nitrative modifications within the RNA and DNA pools in the phytopathogen structures. In contrast, the protein pool undergoing nitration was diminished confirming that protein tyrosine nitration is a flexible element of the oomycete adaptive strategy to heavy metal stress. Finally, to verify whether Cd is able to modify P. infestans pathogenicity, a disease index and molecular assessment of disease progress were analysed indicating that Cd stress enhanced aggressiveness of vr P. infestans towards various potato cultivars. Taken together, Cd not only affected hyphal growth rate and caused biochemical changes in P. infestans structures, but accelerated the pathogenicity as well. The nitro-oxidative homeostasis imbalance underlies the phytopathogen adaptive strategy and survival in the heavy metal contaminated environment. |
format | Online Article Text |
id | pubmed-7664633 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76646332020-11-14 Cadmium Stress Reprograms ROS/RNS Homeostasis in Phytophthora infestans (Mont.) de Bary Gajewska, Joanna Azzahra, Nur Afifah Bingöl, Özgün Ali Izbiańska-Jankowska, Karolina Jelonek, Tomasz Deckert, Joanna Floryszak-Wieczorek, Jolanta Arasimowicz-Jelonek, Magdalena Int J Mol Sci Article Heavy metal pollution causes many soils to become a toxic environment not only for plants, but also microorganisms; however, little is known how heavy metal contaminated environment affects metabolism of phytopathogens and their capability of infecting host plants. In this study the oomycete Phytophthora infestans (Mont.) de Bary, the most harmful pathogen of potato, growing under moderate cadmium stress (Cd, 5 mg/L) showed nitro-oxidative imbalance associated with an enhanced antioxidant response. Cadmium notably elevated the level of nitric oxide, superoxide and peroxynitrite that stimulated nitrative modifications within the RNA and DNA pools in the phytopathogen structures. In contrast, the protein pool undergoing nitration was diminished confirming that protein tyrosine nitration is a flexible element of the oomycete adaptive strategy to heavy metal stress. Finally, to verify whether Cd is able to modify P. infestans pathogenicity, a disease index and molecular assessment of disease progress were analysed indicating that Cd stress enhanced aggressiveness of vr P. infestans towards various potato cultivars. Taken together, Cd not only affected hyphal growth rate and caused biochemical changes in P. infestans structures, but accelerated the pathogenicity as well. The nitro-oxidative homeostasis imbalance underlies the phytopathogen adaptive strategy and survival in the heavy metal contaminated environment. MDPI 2020-11-08 /pmc/articles/PMC7664633/ /pubmed/33171629 http://dx.doi.org/10.3390/ijms21218375 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gajewska, Joanna Azzahra, Nur Afifah Bingöl, Özgün Ali Izbiańska-Jankowska, Karolina Jelonek, Tomasz Deckert, Joanna Floryszak-Wieczorek, Jolanta Arasimowicz-Jelonek, Magdalena Cadmium Stress Reprograms ROS/RNS Homeostasis in Phytophthora infestans (Mont.) de Bary |
title | Cadmium Stress Reprograms ROS/RNS Homeostasis in Phytophthora infestans (Mont.) de Bary |
title_full | Cadmium Stress Reprograms ROS/RNS Homeostasis in Phytophthora infestans (Mont.) de Bary |
title_fullStr | Cadmium Stress Reprograms ROS/RNS Homeostasis in Phytophthora infestans (Mont.) de Bary |
title_full_unstemmed | Cadmium Stress Reprograms ROS/RNS Homeostasis in Phytophthora infestans (Mont.) de Bary |
title_short | Cadmium Stress Reprograms ROS/RNS Homeostasis in Phytophthora infestans (Mont.) de Bary |
title_sort | cadmium stress reprograms ros/rns homeostasis in phytophthora infestans (mont.) de bary |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7664633/ https://www.ncbi.nlm.nih.gov/pubmed/33171629 http://dx.doi.org/10.3390/ijms21218375 |
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